1st Assignment of Biochemistry For 2nd Year Students (1211213)

Student Name: …………………………….

Aljory abdulaziz almejlad
Student ID: ………………………………..
202200291
Task 1:
This a DNA fragment of β- globin gene:

3' CAC GTG GAC TGA GGA CAC CTC TTC AGA CGG CAA TGA CGG GAC 5'
1- Give the complementary strand of this DNA sequence (5’-3’)
5’ GTG CAC CTG ACT CCT GTG GAG AAG TCT GCC GTT ACT GCC
……………………………………………………………………………………………………………………………….
CTG 3’
..……………………………………………………………………………………………………………………………..
………………………………………………………………………………………………………………………….......

2- Knowing that the given 3’ - 5’ strand is the template, give the mRNA
sequence (5’-3’)

5’ GUG CAC CUG ACU CCU GUG GAG AAG UCU GCC GUU ACU GCC
……………………………………………………………………………………………………………………………….
CUG 3’
..……………………………………………………………………………………………………………………………..
………………………………………………………………………………………………………………………….......

3- The process of copying genetic information from one strand of DNA to

RNA is called:
TRANSCRIPTION
……………………………………………………………………………………………………………………………….

4- Using the genetic code, give the protein sequence coded by this DNA
fragment:

VALINE - HISTIDINE – LEUCINE –THREONINE – PROLINE – VALINE –

……………………………………………………………………………………………………………………………….
GLUAMATE – LYSINE - SERINE - ALANINE - LEUCINE -
..……………………………………………………………………………………………………………………………..
THREONINE – ALANINE- LEUCINE
………………………………………………………………………………………………………………………….......
.................................................................................................................................
 5- The process by which the genetic code contained within a mRNA
molecule is decoded to produce a specific sequence of amino acids is
called:
TRANSLATION
……………………………………………………………………………………………………………………………….

6- What is the 6th amino acid in this protein sequence? Is the subject with
this DNA sequence of β- globin gene healthy or affected by a genetic
disorder?
VALINE IT IS AFFECTED BY GENETIC DISORDER
……………………………………………………………………………………………………………………………….

7- If this subject is affected, specify the type of hemoglobinopathy and

precise the mutation?

SICKLE CELL DISEASE WHERE GLUTAMATE IN THE 6TH POSITION IS REPLACED BY

……………………………………………………………………………………………………………………………….
VALINE