FLOW RATE LINEARITY REPORT

ACQUI SI T I ON I NF ORMAT I ON

SystemName: IClass_BSM_FTN_PDA_ELS Acquired By: Natasha

Project Name: SQT_E_BSM_FTN_PDA_ELSD Column serial Number: 017139167157 42
Sample Set Method: 070 Flow Rate Linearity SSM Sample Kit 1 Expiration Date: 11/23/2011 12:00:00 AM EST
Processing Method Flow Rate Linearity PM Sample Kit 1 Lot Number: W04040902
Report Method Name: Flow Rate Linearity RM Build Version: Empower 2 Software Build 2154 SPs Installed: Service
User Name: Lisa Busher (lbusher) Sample Set Id: 1813 Result Set Id: 1824
Sample Set Name: Flow Rate Linearity SSM Sample Set Start Date: 11/23/2011 4:08:39 PM EST

The Flow Rate Linearity qualification measures the Retention Time of Caffeine at varyingFlowRates
and plots the reciporical of the Retention Time vs. Flow Rate (mL/min).

The qualification passes if the plot of 1/RT vs. Flow Rate has an R^2 value of > or = 0.990.

Flow Rate Linearity Results Table

LinearityR^2 Specification LinearityR^2 LinearityR^2 Pass Fail

1 R^2 > or = 0.990 1.000 PASS

Printed 6:42:17 PM US/Eastern 1/16/2012 Page: 1 of 3

 FLOW RATE LINEARITY REPORT
ACQUI SI T I ON I NF ORMAT I ON

SystemName: IClass_BSM_FTN_PDA_ELS Acquired By: Natasha

Project Name: SQT_E_BSM_FTN_PDA_ELSD Column serial Number: 017139167157 42
Sample Set Method: 070 Flow Rate Linearity SSM Sample Kit 1 Expiration Date: 11/23/2011 12:00:00 AM EST
Processing Method Flow Rate Linearity PM Sample Kit 1 Lot Number: W04040902
Report Method Name: Flow Rate Linearity RM Build Version: Empower 2 Software Build 2154 SPs Installed: Service
User Name: Lisa Busher (lbusher) Sample Set Id: 1813 Result Set Id: 1824
Sample Set Name: Flow Rate Linearity SSM Sample Set Start Date: 11/23/2011 4:08:39 PM EST

Overlay of Caffeine Peak Chromatograms

2.50

2.00

1.50

Caffeine - 3.905
Caffeine - 1.969
Caffeine - 1.001
Caffeine - 0.680
AU

1.00

0.50

0.00

0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00

Minutes
Flow 0.6 ml per min
Flow 0.4 ml per min
Flow 0.2 ml per min
Flow 0.1 ml per min

Flow Rate Linearity Results Table

Peak: Caffeine
RT Height Area
Vial SampleName Amount Level Reciprocal_RetnTime X Value Channel Id Result Id
(min) (µV) (µV*sec)
1 2:E,4 Flow0.6 ml per min 0.6 1 0.680 1.4697 0.6000 1085118 1245371 1816 1857
2 2:E,4 Flow0.4 ml per min 0.4 2 1.001 0.9994 0.4000 1164414 1877837 1827 1858
3 2:E,4 Flow0.2 ml per min 0.2 3 1.969 0.5080 0.2000 1171324 3775491 1837 1859
4 2:E,4 Flow0.1 ml per min 0.1 4 3.905 0.2561 0.1000 1076203 7572648 1847 1860

Printed 6:42:17 PM US/Eastern 1/16/2012 Page: 2 of 3

 FLOW RATE LINEARITY REPORT
ACQUI SI T I ON I NF ORMAT I ON

SystemName: IClass_BSM_FTN_PDA_ELS Acquired By: Natasha

Project Name: SQT_E_BSM_FTN_PDA_ELSD Column serial Number: 017139167157 42
Sample Set Method: 070 Flow Rate Linearity SSM Sample Kit 1 Expiration Date: 11/23/2011 12:00:00 AM EST
Processing Method Flow Rate Linearity PM Sample Kit 1 Lot Number: W04040902
Report Method Name: Flow Rate Linearity RM Build Version: Empower 2 Software Build 2154 SPs Installed: Service
User Name: Lisa Busher (lbusher) Sample Set Id: 1813 Result Set Id: 1824
Sample Set Name: Flow Rate Linearity SSM Sample Set Start Date: 11/23/2011 4:08:39 PM EST

Flow Rate Linearity Results

Calibration Id 1855 Y Intercept 0.019443

Equation Y = 2.43e+000 X+ 1.94e-002 Slope 2.427244

Fit Type Linear (1st Order) R^2 0.9998

Caffeine Flow Rate Linearity Calibration Plot

1.50
Reciprocal_RetnTime

1.00

0.50

0.00
0.00 0.10 0.20 0.30 0.40 0.50 0.60
R^2 0.9998

Flow Rate (ml/min)

Printed 6:42:17 PM US/Eastern 1/16/2012 Page: 3 of 3