CARRYOVER REPORT

ACQUI SI TI ON I NFORMATI ON

SystemName: IClass_BSM_FTN_PDA_ELS Acquired By: Natasha

Project Name: SQT_E_BSM_FTN_PDA_ELSD Column serial Number: 017139167157 42
Sample Set Method: 080 Carryover SSM Sample Kit 1 Expiration Date: 11/23/2011 12:00:00 AM EST
Processing Method Carryover Clean PM, Carryover PM Sample Kit 1 Lot Number: W04040902
Report Method Name: Carryover RM Build Version: Empower 2 Software Build 2154 SPs Installed: Service
User Name: Lisa Busher (lbusher) Sample Set Id: 1861 Result Set Id: 1872
Sample Set Name: Carryover SSM Sample Set Start Date: 11/23/2011 4:41:06 PM EST

In the Carryover qualification, two pre-blank injections are performed to determine if the chromatograph is
cleanenough to measure carryover. Those injections are followed by three Caffeine calibrationinjections at a
concentration of (0.2ppm) whichis 0.005 percent of the Challenge injection concentration (4000 ppm).

The Challenge injection is made and is followed bythree post-blank injections.

Carryover is reported as a percent of the Challenge concentration. If the % Carryover of first post-blank injection
is < or = 0.005%the qualification passes.

Carryover Peak Results

Carryover Carryover
SampleName Peak Type %Carryover
Specification Pass Fail
1 PostBlank1 < or = 0.005% Missing PASS

Printed 6:42:59 PM US/Eastern 1/16/2012 Page: 1 of 3

 CARRYOVER REPORT

ACQUI SI TI ON I NFORMATI ON

SystemName: IClass_BSM_FTN_PDA_ELS Acquired By: Natasha

Project Name: SQT_E_BSM_FTN_PDA_ELSD Column serial Number: 017139167157 42
Sample Set Method: 080 Carryover SSM Sample Kit 1 Expiration Date: 11/23/2011 12:00:00 AM EST
Processing Method Carryover Clean PM, Carryover PM Sample Kit 1 Lot Number: W04040902
Report Method Name: Carryover RM Build Version: Empower 2 Software Build 2154 SPs Installed: Service
User Name: Lisa Busher (lbusher) Sample Set Id: 1861 Result Set Id: 1872
Sample Set Name: Carryover SSM Sample Set Start Date: 11/23/2011 4:41:06 PM EST

Caffeine
0.004 0.004 0.004
AU

AU

AU
Caffeine

Caffeine
0.002 0.002 0.002

0.000 0.000 0.000

0.00 0.50 1.00 0.00 0.50 1.00 0.00 0.50 1.00

Minutes Minutes Minutes

PreBlank; Vial: 2:A,1; Inj: 1 PreBlank; Vial: 2:A,1; Inj: 2 Standard; Vial: 2:A,2; Inj: 1
Caffeine

Caffeine

0.004 0.004 0.004

AU

AU

AU

0.002 0.002 0.002

0.000 0.000 0.000

0.00 0.50 1.00 0.00 0.50 1.00 0.00 0.50 1.00

Minutes Minutes Minutes

Standard; Vial: 2:A,2; Inj: 2 Standard; Vial: 2:A,2; Inj: 3 Challenge; Vial: 2:A,3; Inj: 1

0.004 0.004 0.004

AU

AU

AU
Caffeine

Caffeine

Caffeine

0.002 0.002 0.002

0.000 0.000 0.000

0.00 0.50 1.00 0.00 0.50 1.00 0.00 0.50 1.00

Minutes Minutes Minutes

PostBlank1; Vial: 2:A,4; Inj: 1 PostBlank2; Vial: 2:A,5; Inj: 1 PostBlank3; Vial: 2:A,6; Inj: 1

Printed 6:42:59 PM US/Eastern 1/16/2012 Page: 2 of 3

 CARRYOVER REPORT

ACQUI SI TI ON I NFORMATI ON

SystemName: IClass_BSM_FTN_PDA_ELS Acquired By: Natasha

Project Name: SQT_E_BSM_FTN_PDA_ELSD Column serial Number: 017139167157 42
Sample Set Method: 080 Carryover SSM Sample Kit 1 Expiration Date: 11/23/2011 12:00:00 AM EST
Processing Method Carryover Clean PM, Carryover PM Sample Kit 1 Lot Number: W04040902
Report Method Name: Carryover RM Build Version: Empower 2 Software Build 2154 SPs Installed: Service
User Name: Lisa Busher (lbusher) Sample Set Id: 1861 Result Set Id: 1872
Sample Set Name: Carryover SSM Sample Set Start Date: 11/23/2011 4:41:06 PM EST

Pre-Blank Injections

SampleName Sample Type Vial Injection Name RT Area Height Peak Type %Carryover
1 PreBlank Unknown 2:A,1 1 Caffeine 0.700 Missing
2 PreBlank Unknown 2:A,1 2 Caffeine 0.700 Missing

Caffeine Standards

SampleName Sample Type Vial Injection Name RT Area Height Peak Type %Carryover
1 Standard Standard 2:A,2 1 Caffeine 0.679 5088 4424 Found 0.0050
2 Standard Standard 2:A,2 2 Caffeine 0.679 5114 4468 Found 0.0050
3 Standard Standard 2:A,2 3 Caffeine 0.679 5072 4469 Found 0.0050

Post-Blank Injections

SampleName Sample Type Vial Injection Name RT Area Height Peak Type %Carryover
1 PostBlank1 Unknown 2:A,4 1 Caffeine 0.700 Missing
2 PostBlank2 Unknown 2:A,5 1 Caffeine 0.700 Missing
3 PostBlank3 Unknown 2:A,6 1 Caffeine 0.700 Missing

Printed 6:42:59 PM US/Eastern 1/16/2012 Page: 3 of 3