Name: Peligres, Pornela, Portugal, Saito, Tomas, Tulayba Rating:

Course, Year and Section: BSMT2H Instructor: Prof. Ranola

Group Number: 5 Date: November 9, 2023

Specific Reactions of Carbohydrates

EXPERIMENT 4
A. Bial’s Orcinol Test

Test solution Reagents Result Interpretation

Bial’s Reagent orange → muddy brown NEGATIVE TEST. Resulting
1% glucose
color color indicates the presence
of hexose sugar.
Bial’s Reagent orange → dark brown color NEGATIVE TEST. Resulting
1% fructose
color indicates the presence
of hexose sugar.
Bial’s Reagent orange → bluish green color POSITIVE TEST. Resulting
1% xylose
color indicates the presence
of pentose sugar.
Unknown solution 1
Unknown solution 2

B. Seliwanoff’s Test

Test solution Classification Result Interpretation

1% fructose ketose faint yellow → Positive test. Resulting color indicates

orangey-yellow color the presence of ketose.

1% sucrose ketose faint yellow → Positive test. Resulting color indicates

orangey-yellow color the presence of ketose.

1% starch aldose no reaction Negative test

1% glucose aldose no reaction Negative test

Note: There is a deviation from the typical cherry red color associated with the presence of ketose.

C. Test for Complex Carbohydrates - Iodine Test

Test solution Reagents Result Interpretation
1% starch Iodine Solution Pale yellow Negative Result
1% glycogen Iodine Solution Light Brown Negative
1% glucose Iodine Solution Clear Negative Result