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    Jhana Samson
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    Nematodes-Lab

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    8 views12 pages

    Nematodes Lab

    Uploaded by

    Jhana Samson

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 12

    Nematodes

    Ascaris lumbricoides
    • Ova
    • Fertilized (45-70 um X 35-50um) vs. Unfertilized (88-94 um X 39-44
    um)
    • 2-3 layers (Mammillary Albumin coat, Glycogen layer, Vitelline
    lipoidal layer)
    • Adult
    • Long with 3 oval lips
    Trichuris trichiura/Trichocephalus
    trichiurus
    • Ova
    • Barrel or football shaped with BIPOLAR MUCOUS PLUGS (50-
    54um X 23um)
    • “JAPANESE LANTERN OVA”
    • Adult
    • Slender anterior and fleshy posterior
    Enterobius vermicularis
    Oxyuris vermicularis
    • Ova
    • D shaped, double walled/layered, flattened on one end (50-60um
    X 20-30um)
    • Thin transparent shell and occasionally contains the LARVA
    • Adult
    • Female worms have the distinct “cephalic alae”
    Capillaria philippinensis
    • Ova (36-45um x 20um)
    • Peanut shaped, double walled, striated with bipolar plugs
    • Adult
    • Delicate tiny worms, male have chitinized spicules
    Stronglyoides stercoralis
    THREADWORM
    • Adult
    • Parasitic female is longer than free living
    • Parthenogenesis
    • Male are smaller with two spicules
    • Egg (50-58um x 30-34um)
    • “Chinese lantern ova”
    Hookworms
    • Adult:
    • Male: Copulatory bursa
    • Female : straight tail
    • Eggs:
    • Hookworm eggs are IDENTICAL (reported only as “hookworm
    egg”)
    • Thin walled with 2-8 germ cells
    Rhabditiform larva

    Hookworms S. stercoralis
    • Long buccal cavity • Short buccal cavity
    • Small genital primordium • Large genital primordium
    Filariform Larva

    Hookworms S. stercoralis
    • Short esophagus • Long esophagus
    • Pointed and sheathed tail • Notched and unsheathed tail
    Morphology of the Diagnostic Stage
    • DIAGNOSTIC STAGE OF FILARIAL WORMS: MICROFILARIA
    • Basis: Presence of sheath, Terminal nuclei (tail)
    SHEATH TERMINAL NUCLEI

    Brugia malayi 2 distinct terminal nuclei


    Wuchereria bancrofti SHEATHED No terminal nuclei
    Loa loa Extended terminal nuclei
    Dipetalonema (Mansonella)
    Extended terminal nuclei
    perstans
    Onchocerca volvulus UNSHEATHED No terminal nuclei
    Mansonella ozzardi No terminal nuclei
    Morphology of the Diagnostic Stage
    • DIAGNOSTIC STAGE OF FILARIAL WORMS: MICROFILARIA
    • Basis: Presence of sheath, Terminal nuclei (tail)
    SHEATH TERMINAL NUCLEI

    Brugia malayi 2 distinct terminal nuclei


    Wuchereria bancrofti SHEATHED No terminal nuclei
    Loa loa Extended terminal nuclei
    Dipetalonema (Mansonella)
    Extended terminal nuclei
    perstans
    Onchocerca volvulus UNSHEATHED No terminal nuclei
    Mansonella ozzardi No terminal nuclei

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