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A Practical Approach to Using Statistics
in Health Research
A Practical Approach to Using Statistics
in Health Research
Adam Mackridge
Philip Rowe
This edition first published 2018
© 2018 John Wiley & Sons, Inc.
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Library of Congress Cataloguing-in-Publication Data
Names: Mackridge, Adam (Adam John), 1979- author. | Rowe, Philip, author.
Title: A practical guide to statistics for health research / by Adam
Mackridge, Philip Rowe.
Description: Hoboken, NJ : Wiley, 2018. | Includes bibliographical references
and index. |
Identifiers: LCCN 2017055955 (print) | LCCN 2018001635 (ebook) | ISBN
9781119383598 (pdf ) | ISBN 9781119383611 (epub) | ISBN 9781119383574
(hardback)
Subjects: LCSH: Medical statistics. | Medicine–Research–Statistical
methods. | BISAC: MEDICAL / Epidemiology. | MATHEMATICS / Probability &
Statistics / General.
Classification: LCC R853.S7 (ebook) | LCC R853.S7 M33 2018 (print) | DDC
610.2/1–dc23
LC record available at https://lccn.loc.gov/2017055955
Cover Design: Wiley
Cover Image: © Tetra Images/Getty Images
Set in 10/12pt WarnockPro by SPi Global, Chennai, India
Printed in the United States of America
10 9 8 7 6 5 4 3 2 1
v
Contents
1 Introduction 1
1.1 At Whom is This Book Aimed? 1
1.2 At What Scale of Project is This Book Aimed? 2
1.3 Why Might This Book be Useful for You? 2
1.4 How to Use This Book 3
1.5 Computer Based Statistics Packages 4
1.6 Relevant Videos etc. 5
2 Data Types 7
2.1 What Types of Data are There and Why Does it Matter? 7
2.2 Continuous Measured Data 7
2.2.1 Continuous Measured Data – Normal
and Non‐Normal Distribution 8
2.2.2 Transforming Non‐Normal Data 13
2.3 Ordinal Data 13
2.4 Categorical Data 14
2.5 Ambiguous Cases 14
2.5.1 A Continuously Varying Measure that has been Divided
into a Small Number of Ranges 14
2.5.2 Composite Scores with a Wide Range of Possible Values 15
2.6 Relevant Videos etc. 15
4 27
Choosing a Statistical Test
4.1 Identify the Factor and Outcome 27
4.2 Identify the Type of Data Used to Record the Relevant
Factor 29
4.3 Statistical Methods Where the Factor
is Categorical 30
4.3.1 Identify the Type of Data Used to Record
the Outcome 30
4.3.2 Is Continuous Measured Outcome Data
Normally Distributed or Can It Be Transformed
to Normality? 30
4.3.3 Identify Whether Your Sets of Outcome Data Are Related
or Independent 31
4.3.4 For the Factor, How Many Levels Are Being Studied? 32
4.3.5 Determine the Appropriate Statistical Method for Studies
with a Categorical Factor 32
4.4 Correlation and Regression with a Measured Factor 34
4.4.1 What Type of Data Was Used to Record Your Factor
and Outcome? 34
4.4.2 When Both the Factor and the Outcome Consist
of Continuous Measured Values, Select Between Pearson
and Spearman Correlation 34
4.5 Relevant Additional Material 38
Contents vii
5 Multiple Testing 39
5.1 What Is Multiple Testing and Why
Does It Matter? 39
5.2 What Can We Do to Avoid an Excessive Risk of False
Positives? 40
5.2.1 Use of Omnibus Tests 40
5.2.2 Distinguishing Between Primary and Secondary/
Exploratory Analyses 40
5.2.3 Bonferroni Correction 41
9 Mann–Whitney Test 83
9.1 When Is the Test Applied? 83
9.2 An Example 83
9.3 Presenting the Data 85
9.3.1 Numerically 85
9.3.2 Graphically 85
9.3.3 Divide the Outcomes into Low and High Ranges 85
9.4 Data Requirements 86
9.4.1 Variables Required 86
9.4.2 Normal Distributions and Equality of Standard
Deviations 87
9.4.3 Equal Sample Sizes 87
9.5 An Outline of the Test 87
9.6 Statistical Significance 87
9.7 Planning Sample Sizes 87
9.8 Carrying Out the Test 88
9.9 Describing the Effect Size 88
9.10 How to Report the Test 89
9.10.1 Methods Section 89
9.10.2 Results Section 89
9.10.3 Discussion Section 90
9.11 Relevant Videos etc. 91
11 Kruskal–Wallis 105
11.1 When Is the Test Applied? 105
11.2 An Example 105
11.3 Presenting the Data 106
11.3.1 Numerically 106
11.3.2 Graphically 107
11.4 Data Requirements 109
11.4.1 Variables Required 109
11.4.2 Normal Distributions and Standard Deviations 109
11.4.3 Equal Sample Sizes 110
11.5 An Outline of the Test 110
11.6 Planning Sample Sizes 110
11.7 Carrying Out the Test 110
11.8 Describing the Effect Size 111
11.9 Determining Which Group Differs
from Which Other 111
11.10 How to Report the Test 111
11.10.1 Methods Section 111
11.10.2 Results Section 112
11.10.3 Discussion Section 113
11.11 Relevant Videos etc. 114
Glossary 197
Videos 209
Index 211
xv
www.wiley.com/go/Mackridge/APracticalApproachtoUsing
StatisticsinHealthResearch
Introduction
Table 1.1 The ideal stage‐by‐stage flow of events for a research program.
provided the data in Microsoft Excel format. If you do not have access
to this program either, you can download a free Excel Viewer program
from Microsoft’s website that will allow you to view the data sets.
Unfortunately, despite its considerable price, SPSS does not calculate
necessary sample sizes. We therefore also refer to G*Power, which will
do this job. G*Power is free software that can be downloaded from the
internet – we would advise using the Heinrich‐Heine‐Universität
Düsseldorf site.
Data Types
the reality is that pressure could be 91.25 mmHg (or any figure with
an unlimited number of decimal places) – we just choose not to
measure to this degree of precision.
●● There is a large number of possible different values that might be
recorded. For example, diastolic blood pressures typically vary over
a range of 60 to 120 mmHg, giving 61 different recorded values.
●● Each step up the scale of one unit is of equal size. E.g. the difference
between pressures of 80 and 81 mmHg is exactly the same as that
between 94 and 95 mmHg.
1
Rowe P. Essential statistics for the pharmaceutical sciences, 2nd edn. Chichester:
Wiley, 2015.
2.2 Continuous Measured Dat 9
20
15
Frequency
10
0
20 24 28 32 36 40 44
Measured value
(a)
25
20
Frequency
15
10
0
0 15 30 45 60 75 90
Measured value
(b)
Figure 2.1 A continuously varying measure with (a) normal, (b) skewed, and
(c) bimodal distribution. In (d) the highest and lowest values (tails) from an
otherwise normal distribution are missing.
10 2 Data Types
50
40
Frequency
30
20
10
0
0 15 30 45 60 75 90
Measured value
(c)
40
30
Frequency
20
10
0
48 52 56 60 64 66 72
Measured value
(d)
Figure 2.1d shows a final form of non‐normality. Results are cut off
suddenly at values of around 50 and 70; for a true normal distribu-
tion, there should be a more gradual decline in frequencies beyond
these limits.
NOTE: Do not expect to get ideal bell‐shaped distributions, especially
with small samples. The distribution shown in Figure 2.1a is perfectly
acceptable. We just don’t want to see obvious deviations from normal-
ity such as seen in Figure 2.1b, c, or d.
2.2 Continuous Measured Dat 11
140
120
100
Frequency
80
60
40
20
0
−9.0 −4.5 0.0 4.5 9.0 13.5 18.0
Measured value
Figure 2.2 Histogram of “Long‐tailed” data, i.e. data that includes both low and
high outlying values.
99.9
99
Theoretically expected value
95
90
80
70
60
50
40
30
20
10
5
0.1
0 1 2 3 4 5 6 7
Observed value
(a)
99.9
99
Theoretically expected value
95
90
80
70
60
50
40
30
20
10
5
0.1
−10 −5 0 5 10 15 20
Observed value
(b)
Figure 2.3 Normal probability plots of (a) normally distributed data and
(b) long‐tailed data.
data produces a normal probability plot like that shown in Figure 2.3b,
then you should treat your data as non‐normal. In further chapters
that discuss methods requiring normality, you will be advised how to
handle non‐normality.
2.3 Ordinal Dat 13
See the video listed at the end of the chapter for details of using a
statistical package to check data normality.
2.3 Ordinal Data
Here, the characteristic to be measured is often subjective in nature.
For example, we might assess how patients feel about a treatment they
have received, using a score, of (say) one to five with the following
equivalences:
1 = Strongly dissatisfied
2 = Somewhat dissatisfied
3 = Neither satisfied nor dissatisfied
4 = Generally satisfied
5 = Very satisfied
The data consists of categorizations, but the important thing is that the
categories have a natural order to them. There is a definite ranking from
“Strongly dissatisfied” to “Very satisfied” via three intermediate grades.
This type of data has the following characteristics:
●● It is discontinuous. Only these five integer values are available.
There are no scores of 1.45 or any other fractional value.
●● Ordinal scales usually allow only small numbers of possible values
(five in the current case).
14 2 Data Types
●● It often cannot be assumed that all the steps up the scale are of equal
significance. Although you may have scored gradings as 1,2,3 etc.,
you cannot assume that the difference between “Strongly dissatisfied”
and “Somewhat dissatisfied” is of exactly the same importance as
that between “Somewhat dissatisfied” and “Neither satisfied nor
dissatisfied.”
2.4 Categorical Data
This is also known as “Nominal” data. Here there is no intention to meas-
ure a characteristic: we are just categorizing. For example, advice might
be provided by nurses, pharmacists, or physiotherapists. These do not
form any sort of scale; they are just three different professions. Frequently
there are just two options, obvious cases being Male/Female, Yes/No or
Successful/Failed; these are referred to as “Binary” or “Dichotomous.”
2.5 Ambiguous Cases
There are a couple of problematic areas where things are not as obvi-
ous as in the examples given above.
In another part of this book I told you something about the moon,
which did not even pretend to be true. No body can go to the moon,
although very many people have traveled more miles than the
distance between the earth and its lunar companion. Any one who
has sailed from New York to Liverpool and back forty times has gone
over a greater distance than that from here to the moon, which is
less than 240,000 miles away.
Many a sea-captain has sailed more miles than these. A ship
came into New York Bay very recently that had sailed, in one voyage
over 110,000 miles.
But we cannot visit the moon because there is no atmospheric air
between that planet and the earth. If air existed in this vast space in
which a balloon might float, and which a man might breathe, I think
that some of us would manage to get to the moon before any one
reaches the North Pole. The journey would be longer, but there
would be no ice to block up the way.
But notwithstanding the fact that we cannot go to the moon, we
know a great deal about that planet, especially as it affects the earth.
And with the great telescopes that have been constructed, in late
years, we can see much of the general configuration of that side of
the moon which is turned towards us, and it appears very like the
picture at the head of this article. Here we see depressions and
elevations, and plain surfaces which may be, and probably are,
mountains and beds of dried up oceans and vast plains, which, in all
probability, are barren and desolate.
For scientific men feel quite certain that the moon has no
atmosphere, and of course if there is no air, we have no reason to
suppose that there is any life there.
But our principal interest in the moon relates to its effects upon
ourselves, and our own planet, and therefore we should all
understand it as we see and enjoy it from our stand-point.
We all know that sometimes the moon is full and bright, flooding
the earth with its lovely light, and that, at other times it is quite dim,
just a curved strip of light in the sky, and at still other times it seems
to be absent altogether.
Though we have noticed all this, it is very probable indeed that
some of us do not entirely understand these changes, and so I shall
briefly explain them.
When we cannot see the moon at all, which is the case for two or
three days every month, it is because the sun is not shining on that
side of the moon which is turned to us. And we might as well
remember that although the moon moves around the earth once
every four weeks, it always turns the same side to us. We never
have seen the other side, with telescopes, or in any other way.
When the moon is between us and the sun, the side towards us
must of course be dark. Then it is that we do not see the moon at all.
But as the moon moves gradually to one side we begin to see a
little strip of the bright portion as you notice in Fig. 1. This represents
the moon in its first phase.
I suppose you have noticed at such times, when the new moon is
very small, that we can often see the whole disk of the moon,
although the principal portion of it is very dim indeed. Still we can
see a faint light shining upon it which makes it comparatively easy
for us to discern its outlines.
This pale light is “earth-light.” The earth
is then “full” to the moon-folk, (if there be
any such creatures who can live without
air,) and its brilliancy is partially reflected
back to us from the surface of the moon.
The bright portion of the moon now
grows larger and larger until, in about
seven or eight days, we see it as it is
shown in Figure 2. Then it is said to be in
its first quarter.
FIG. 1. FIRST PHASE OF At this time the spots and various
MOON.
markings on its surface are generally seen
very distinctly.
For seven days the bright portion of the moon continues to grow
larger and larger, and during a part of this time it presents the
appearance shown in Figure 3.
At last, after about fifteen days of active
increase of bright surface the moon
reaches that point where the sun shines
directly upon the side presented to the
earth, and then it is “full moon.” Of course
it must, at this time, be on the side of the
earth farthest from the sun so that the sun
can shine on it, and at the same time, we
can see it.
Figure 4 is a small picture of the full
moon. FIG. 2. FIRST QUARTER.
A NIGHT TEMPEST.
“Then I found that a fearful storm was upon us. The scene had
been entirely changed in an hour. All day it had been calm. There
was not breeze enough to move a sail, and scarcely to ruffle the
water. Now the wind was blowing violently, bending the trees until it
seemed as if they would be torn up by the roots. The air was filled
with whirling leaves. The river was lashed into waves, and white with
foam. The lightning was almost incessant, sometimes in blinding
sheets, and sometimes with flaming lines crossing each other.
“The sloop was flying over the waves as gaily as a bird. There was
not a thread of canvas out. We were rushing along under bare poles.
“The Indian sailors were of no use whatever—worse than useless,
for they were in an agony of terror. They were all in the shrouds and
rigging, holding on for dear life. If it had not been for the pilot, the
captain, and one white sailor, I don’t know what would have become
of us.
“The captain assured me there was no danger, so I secured
myself on deck, and watched the tempest, admiring the fine display it
made, but wishing from my heart it would stop. For I preferred the
certainty of safety to the captain’s assurance that we were safe.
“Fearing that the wind would blow me off the slippery deck, I had
tied one end of a rope around the mast, and the other end around
my waist. I suppose I did not tie it firmly, for the rope slipped from
me; and I felt that I was sliding swiftly over the wet deck, on my way
to the river. The sloop was tipped up at such a high angle I could not
save myself. I gave a shriek; there was a crash; and down I went into
the raging waters!
“For an instant I had no sensation but that of terror. Then the
horror of my situation forced me to think how to save myself. I was in
the midst of this boiling river, at the mercy of the furious waves, and
still more furious wind. The vessel was going at a frantic speed, and
would soon be far away from me. At such a time I would not be
missed; and, even if I were my companions could not save me. They
could do nothing except to throw me a rope, and try to hold me up
until I could get to the ship’s side, when there was a possibility they
might haul me up. But all this was not probable.
“These thoughts flew through my mind in a moment. Then I found I
was close to the ship, but I could not see distinctly. The lightning
flashes seemed to have grown very faint, and everything was
obscure. The only thing I could do was to try to get up the ship’s
side, and I made a desperate clutch at it. My hands failed to get hold
of anything, and down I went into the water again. But I did not sink. I
seemed to be borne up on the top of the waves all the time. But, of
course, I did not reflect upon the strangeness of this. I made another
attempt to scramble up the side of the sloop; and this time, I caught
hold of an iron ring!
“How desperately I clutched it! And how I shrieked for help! But the
gale made such a noise no one heard me. I found to my surprise that
the rolling of the vessel dipped only my feet in the water, and that the
waves did not dash over me. My situation then did not seem to be
quite so hopeless, and I redoubled my cries for help.
“Soon I heard the gruff voice of the Captain calling down to me:
‘What is the matter there?’”
“‘I am overboard, and drowning,’ I shrieked in reply.
“Upon this instead of lowering a rope, he extended me his hands,
and helped me up—out of the cabin!
“The crash I had heard was the cabin hatchway, and I had fallen
through it into the room below, and into about two feet of water! The
iron ring was a hammock ring. No wonder I did not see the lightning
flashes down in that hole.
“The raging waves, and the tossing about in the wind, and the
vessel scudding away from me had only existed in my imagination. I
was so certain that I had fallen into the river that I imagined the rest.
“The tempest soon ceased, but not the laugh at me. That lasted all
the way to Para. Somehow my adventure seemed more ridiculous to
the Captain and the sailors than it did to me!
STILL WATERS.
“We had lovely weather during the remainder of our trip. Our Bella
Donna behaved very well except that she would get on a sand-bar
occasionally. This was partly the pilot’s fault, and partly the fault of
the river in having so many sand-bars.
‘Did not the Captain try to get his sloop off the sand-bars?’ George
inquired.
“Yes, the sailors would try to work the vessel off, sometimes
getting into the water, and working like Trojans. But they never did
get her off; and we would just stick there until the next tide which
invariably floated us on our way.
“I took advantage of these detentions to visit the shores, and
explore the country. In this way I became acquainted with some very
strange Indian tribes. But I saw nothing of the Amazons—the female
warriors you have read of. Nor did I meet with any one who ever had
seen any of them. I did find a ruined fortress, but I never heard that
the Amazons had anything to do with fortresses. They trusted to their
bows and spears.
“In the place of these warlike females are gentle, inoffensive
Indian women, who will sell you delicious fruits, or make you a
hammock for a small sum of money, or a few ornaments.
“Immense forests stretch along both banks, filled with luxuriant
vegetation. To a resident, a trip on the river, sailing between these
lines of forests, is tedious, and monotonous. But a stranger is
constantly interested in the beautiful and wonderful plants around
him.
“There are also farms along the river, and occasionally a white
settlement—usually a village; rarely a town.
“And, if the vessel gets on sand-bars as often as our good sloop,
the Bella Donna, the stranger will have an opportunity of seeing
some of the animals of the country, Some of them are not very
pleasant to meet, especially the jaguar. But the monkeys are
amusing.
“At some of the mission stations among the Indians he will also
see a good deal to interest him. The Indians have been taught
something of agriculture, and have some very primitive machines.
“In one of my rambles I came across a sugar-cane mill, in which
three Indians were at work.
SUGAR-CANE MILL.
“It was an enormous affair, but worked entirely by man-power. The
great wheels were made to revolve by a single man working in each,
very much in the style in which squirrels turn toy wheels in their
cages.
“With the exception of my sad adventure during the tempest, I
enjoyed the ten days’ trip on the Bella Donna very much. But ten
days is enough for such a journey, and I was not sorry when I
reached Para, and the sea coast.”
THE BEDOUIN ARABS.
Fine stories are told of the Bedouin Arabs. We have heard a great
deal about these wandering tribes of the desert; of their hospitality to
strangers; of their generosity; of their gratitude; of their affection for
their fleet and beautiful horses; of the wild free life they lead. They
will not allow themselves to be cooped up in towns, they will not
even live in houses, but spend their lives in breezy tents, out on the
wilds.
When girls and boys read these accounts their hearts glow at the
thought of the happy life of the Arab children. No lessons to learn, no
school to attend, no work to do. They course around on splendid
horses, and their whole life is one delightful “camping out.” When
they get tired of living in one place, they go to another. They dress
gorgeously too! A loose, and gracefully flowing costume, made of
“rich stuffs,” and costly camel’s hair cloth.
And then besides the horse they have the docile and intelligent
camel to bear their burdens, and to be their companions.
Such are the pictures often drawn of Bedouin life; and, no doubt
you have thought when you read them, that if these ignorant, lazy,
heathen Arabs were so good, and so happy, why should you be sent
to school, and taught to be industrious, and trained to follow the
precepts of the Bible? To be good, and to be happy are certainly the
main things, and if these Arabs have learned the secret of
commanding these we had better take lessons from them.
Let us look at the accounts of reliable travelers, and see how far
the descriptions of the story-writers are true.