BC-5800 Liquid System

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Liquid System
Components
Counting Principle
Counting Sequence
Troubleshooting

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Components

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 Components
Solenoid valve 22 3-way Mindray valves, 26 2-way
Mindray valves, 4 2-way ASCO valve,
Valve
1 SMC valve ( 2-way valve)
Pinch valve 3 small tube, 6 big tube
Check valve 6 CHV

Metering Pump Metering pump 1 0.32mL, 2 0.52mL, 2 1ml, 1 1.5ml,

1 2mL, 1 2.6mL
Shear valve , Syringe: 100uL, 2.5mL

Sampling Parts Sample probe, Pierce needle, Wipe block

RBC Pre-mixing bath, RBC/PLT bath

Pre-heat bath, WBC Reaction bath, Flow Cell bath, FCM filter
HGB bath
Chamber/Bath
3 Reagent chamber, 3 Waste chamber, Buffer chamber

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SV: Solenoid Valve

New

New New

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Application of Valves

New

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CHV: Check Valve

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Application of Valves

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 SMC valve ( 2-way valve)

New

Note: Pay attention to the direction of tubing connection

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 Application of Valves

New

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PV: Pinch Valve

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Application of Valves

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DP: Diaphragm pump/Metering Pump

1 metering pump + 1 GV=

1 motor + 1 syringe
New

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Application of Metering Pump
LEO(II)
Diff Bath

LEO(I)
Preheat 5

LBA
Preheat 7

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Application of Metering Pump
WC2-2 WC2-1

HGB Bath

Flow Cell

FCM

Diff & Baso

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Application of Metering Pump
SRV

DIL

DIL
New SRV

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Application of Metering Pump

SRV DIL

New

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 Application of Metering Pump
DP1 DP2, 3 DP4 DP5,6 DP7 DP8

Specification 0.32mL 0.52mL 1.5mL 1mL 2mL 2.6mL

DIFF DIFF DIFF/BASO HGB HGB RBC

Usage
LEO(II) LEO(I) sample LH Diluent Diluent

BASO RBC
LBA Premixing

PS: DP2 & DP3, DP5 & DP6 are using different membrane inside, so could not
exchange the position with each other.

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Notice ： when using metering pump
• Liquid port should be toward up in case of some air bubble remain
in the pump.

Liquid port

Gas port

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 Sampling Parts

120ul Blood (WB)

Sample Probe
2.5ml Syringe SRV 120ul Dilution (PD)
Pierce Needle 180ul Blood (WB)

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Sample Probe

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 Pierce Needle

Sample aspirate

internal needle clean (Gas

hole)

external needle clean Blow-dry

Sample aspirate Clean procedure (Sample hole & Gas hole)

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 Syringe

Syringe

Syringe motor Syringe sensor

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 FCM

Application of Syringe
Flow Cell

BASO&DIFF

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Application of Syringe

DIL

 Sample Aspiration
 SRV with Diluent Cleaning

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Shear Valve

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 Shear Valve
• Assembly Chart

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 Manufacture of SRV

• Materials: special alumina ceramic

• Advantage:
1. wearable, stability of chemical character, anti-rust,
good temperature character
2. dispensing fast

• Disadvantage: frangible, hard to machining

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 Shear Valve Components
• Inside New

Sampling valve Rotary valve Rear fixed valve

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 Shear Valve Components
• Difference
• BC5500 SRV,
DIFF&BASO distribution hole=1*20ul
RBC distribution hole=8.3ul
One position fixing hole
• BC5800 SRV,
DIFF&BASO distribution hole=2*10ul
RBC distribution hole=6ul
Two position fixing hole: UP & DOWN position
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 Shear Valve Components
• Sketch Map

Sampling valve Rotary valve Rear fixed valve

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 Function of SRV
• Use flow paths, take the certain length and section to get or
measure the sample
• Ration and shear the blood sample

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 Function of SRV
SRV New
DP1

DP2
DP3
DP8
DP8 DP6
DP7

DP5

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Tubing Connection of SRV

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Work Principle of SRV (1)
Open-vial mode

• The handle on the

sampling valve is
turned to the upper
position. 2.5ml
syringe aspirates
sample into the
shear valve.

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Work Principle of SRV (2)
Open-vial mode

• The handle of the

sampling valve is turned
to the lower position.
The sampling valve
divides the sample into
4 segments and sends
them into different baths
for analysis.

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Work Principle of SRV (3)
Open-vial mode

• The handle of the

sampling valve is on
the lower position.
Certain amount of
primarily diluted
sample is aspirated
from the premix bath.

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Work Principle of SRV (4)
Open-vial mode

• The handle of the

sampling valve is
turned to the upper
position. Certain
amount of primarily
diluted RBC
sample is injected
into the RBC bath.

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Work Principle of SRV (1)
Autoloader/close tube mode

• The handle on the

sampling valve is
turned to the lower
position. 2.5ml
syringe aspirates
sample into the
shear valve.

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Work Principle of SRV (2)
Autoloader/close tube mode

• The handle of the

sampling valve is turned
to the upper position. The
sampling valve divides
the sample into 4
segments and sends
them into different baths
for analysis.

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Work Principle of SRV (3)
Autoloader/close tube mode

• The handle of the

sampling valve is on
the upper position.
Certain amount of
primarily diluted sample
is aspirated from the
premix bath.

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Work Principle of SRV (4)
Autoloader/close tube mode

• The handle of the

sampling valve is
turned to the lower
position. Certain
amount of primarily
diluted RBC sample is
injected into the RBC
bath.

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RBC Pre-mixing Bath & RBC/PLT Bath

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Application of RBC Pre-mixing Bath

RBC Sample In
First Dilution
Out

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Application of RBC/PLT Bath

-0.04Pa
vacuum

New

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 Volumetric Metering
This analyzer uses a volumetric metering unit to control the count
cycle and to ensure that a precise volume of sample is analyzed.

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HGB Bath

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Application of HGB Bath

Lyse HGB sample from SRV

Bubble mixing

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Pre-heat Bath

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Pre-heat Bath

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Application of Pre-heat Bath

SRV-Diff
LEO(I)
LBA
DIL

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WBC Reaction Bath

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WBC Reaction Bath

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Application of WBC Reaction Bath
FCM

LEO1
LBA
LEO2

Flow Cell

WC1

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 Flow Cell Bath

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Application of Flow Cell Bath

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Application of FCM filter
New

Function:
Filter impurity of FCM, protect flow
cell
Shelf life ： 2 year
Attention:
In the installation, inlet port must be
upward

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Reagent, Waste and Buffer Chamber
DIL FCM

WC2

New

Buffer
Cleanse bath
r
WC1
WC3

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Reagent Chamber

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Waste and Buffer Chamber

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 Application of DIL&FCM Chamber

Cleanser Container

DIL Container

Soak the liquid path Generate the sheath Sample dilute and cleaning
when shut down fluid in flow cell

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Application of WC1

WC3

Waste
Container

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Application of WC2
(ASCO Valve
Protection)

WC1

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Application of WC3

WC1

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FAQ1

• What are the 8 metering pumps used for?

• How can we maintain the liquid
components?
• Metering pump, Solenoid valve
• Shear valve
• DIFF/BASO bath, RBC bath, HGB bath
• Flow cell
• Reagent chamber

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FAQ2: How to Check…?

• Sample volume

• Metering pump

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FAQ3: How to Check…?

• Premix/RBC/HGB bath/DIL/FCM chamber

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Counting Principle

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 Counting Principle

WBC channel

• First count Diff channel, get

Lym%, Mon%, Eos%, Neu%

• Then count Baso channel, get

WBC, Baso#, Baso%

• Then get
Lym#, Mon#, Eos#, Neu#

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 Counting Principle
HGB channel
• Colorimetry

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 Counting Principle
RBC/PLT channel
• Electrical Impedance Method

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Counting Sequence

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 Counting Sequence
Flow Chart of Whole Blood Mode

New

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 Counting Sequence
Flow Chart of Prediluted Mode

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Liquid Diagram

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0~11 seconds

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Aspirate sample, test HGB blank, clean & drain (0-11s)
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11~18 seconds

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Dispense sample & lyse, aspirate from pre-mix bath, clean & drain
RBC bath (11-18s) International service department, Mindray.,Co
18~23 seconds

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Dispense into RBC bath, prepare DIFF dilution & Sheath fluid,
HGB dilution stay still(18-23s) International service department, Mindray.,Co
23~34 seconds

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RBC/PLT, DIFF counting, clean pre-mix bath, diff bath, sample
probe & wipe block (23-34s) International service department, Mindray.,Co
34~39 seconds

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RBC/PLT, HGB counting, WBC channel cleaning (34-39s)
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39~45 seconds

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Prepare Baso& Sheath fluid, clean RBC/PLT, HGB bath, prefill pre-mix,
HGB bath; ready for next sample (39-45s) International service department, Mindray.,Co
 45~49 seconds

 Clean the WBC counting module

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Clean RBC rear bath, prefill HGB, DIFF, BASO bath (45-49s)
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49~60 seconds

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Cleaning WBC channel, zap and prefill RBC bath, waste draining
(49-60s) International service department, Mindray.,Co
Maintenance Sequence

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 Cleanser Cleaning
• Cleanser Prime ：
Open SV13 and corresponding valves, prime cleanser by +70kPa
pressure.

• Cleanser Cleaning ：
1.Open SV17, use diluent cleaning corresponding tubings by +70kPa
pressure.
2.Use Metering pump and syringe to clean counting tubing again.

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Cleanser Maintenance
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 Probe Cleanser Cleaning
• It includes SRV, WBC bath, RBC bath, Flow Cell, HGB bath, Premix
bath, HGB bath maintenance.
• Aspiration:
Overall Maintenance: Open SV07, PV10, SV11, SV16 and SV10,
use DP4 aspirate 6ml (4 times) probe cleanser from Open-Vial
probe into WBC channel and flow cell.
SRV Maintenance: Use 2.5ml syringe to aspirate probe cleanser
into SRV.
• Dispensing:
Use 2.5ml syringe dispense probe cleanser into HGB, RBC, Pre-mix
bath.
• Cleaning: it is similar to cleanser cleaning.

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Probe Cleanser Maintenance
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Troubleshooting

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 Abnormal PLT Background

• PLT: 10~40. It is caused by dirty diluent, SRV or tubing.

• Cleaning by background test, probe cleanser soaking and
disassemble cleaning.

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 Abnormal PLT Background
• PLT: If there is interference, usually there will be a peak wave
between 0-10 FL.

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 Abnormal PLT Background
• Check the shielding cover of RBC bath and the signal processing
board whether they are installed well.

• Check or decrease the RBC Gain. After that, you may recalibrate
RBC, PLT and MCV.

• Check there is leakage in the RBC rear bath.

• Check the isolation chamber under the RBC bath whether it is

dirty.

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RBC Bubble
RBC bath not enough diluent?
RBC rear bath leaking?
Anywhere leaking from rear bath to
WC3?
Tube connectors loose?
RBC reference count time setting?
Volumetric sensors work well?

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No WBC Result (about 0.00)
Leucopenia: BASO channel blocked

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 No WBC Result (about 0.00)
• Check the reagent of LBA. Check the liquid parts adding LBA. Such
as metering pump DP3, gas valve GV72, liquid valve SV27 and
tubing from LBA container to SRV.

• Check the metal connectors of WBC reaction bath whether they are
blocked; Clean the SRV and the tubing between the SRV and WBC
reaction Bath.

• Check the pinch valve PV12 and gas valve GV50.

• Check the pressure 0.25MPa, 0.07MPa and -0.085MPa.

• Check the signal processing board.

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Flow cell blocked

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Flow cell inner-surface dirty

Facula before
SS PD

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Flow cell out-surface dirty

Direct light
blocker

Out-surface dirty
Facula before
SS PD

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 Hemolysis insufficient
Lyse reagents, lyse dispense volume, temperature

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Lyse Resist (hemolysis insufficient)

Lyse resist & low LAS Gain

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 Lyse Resist (hemolysis insufficient)

• Check the ambient temperature; it should be much more than 15

centigrade.
• Check the Gain.
• Check the temperature of WBC reaction bath and pre-mix bath.
• Check the lyse LEO(I) and LEO(II); maybe themselves.
• Check the tubing adding LEO(I) and LEO(II) and metal connectors
of WBC reaction bath;
• Clean the SRV and the tubing between the SRV and WBC reaction
Bath.
• Checking the metering pump DP1 and DP2 whether the dispensing
volume is enough. Also the gas valves (such as GV69, 71) and
liquid valves (such as SV25, SV26)
• Check the pressure 0.07MPa and -0.085MPa.

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 HGB Error or HGB Results Abnormal

• Check and adjust HGB Gain to make sure the HGB blank voltage is
2.0-2.4V (2.28V recommended).
• Check the HGB bath whether it is dirty or there is leakage.
• Check the HGB unit whether it is loose or dirty.
• Check whether there is enough diluent dispensed into the HGB bath
when counting. If not, check GV73, and SV19.
• Check whether there is enough LH lyse dispensed into the HGB
bath when counting. If not, check GV70, and SV28.
• Observe the mixing to see if bubble is too big or none. If yes, check
GV65 and CV6.
• Check the pressure 0.07MPa and -0.085MPa.

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 All the stars on the scattergrams
• Check the sheath fluid in the FCM chamber whether it is enough. If
not, perform “Prime > FCM cistern” in the “Service > Maintain”
screen.
• Check the corresponding liquid parts aspirating or dispensing the
diluent; such as SMC valve SV53, liquid valve GV80, tube kinkled or
leaked? Make sure no air going into FCM channel.
• Check the liquid parts dispensing the sheath fluid (diluent) into the
flow cell; such as SV3.

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Thanks!