BC-5800 Liquid System
Hematology Global Technical Support Department
Service@mindray.com
Version ： 1.0
NO.:MXQ-12024-BC-5800
© 2011 Mindray Confidential
OTY-12002 （ 3.0 ）
Liquid System
Components
Counting Principle
Counting Sequence
Troubleshooting
2 © 2011 Mindray Confidential
Components
© 2011 Mindray Confidential
Components
Solenoid valve 22 3-way Mindray valves, 26 2-way Mindray valves, 4
2-way HI-Pressure valve,
Valve
1 SMC valve ( 2-way valve)
Pinch valve 3 small tube, 6 big tube
Check valve 6 CHV
Metering Pump Metering pump 1 0.32mL, 2 0.52mL, 2 1ml, 1 1.5ml,
1 2mL, 1 2.6mL
Shear valve , Syringe: 100uL, 2.5mL
Sampling Parts Sample probe, Pierce needle, Wipe block
RBC Pre-mixing bath, RBC/PLT bath
Pre-heat bath, WBC Reaction bath, Flow Cell bath, FCM filter
Chamber/Bath HGB bath
3 Reagent chamber, 3 Waste chamber, Buffer chamber
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SV: Solenoid Valve
New
New New
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Application of Valves
New
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CHV: Check Valve
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Application of Valves
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SMC valve ( 2-way valve)
New
Note: Pay attention to the direction of tubing connection
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Application of Valves
New
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PV: Pinch Valve
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Application of Valves
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DP: Diaphragm pump/Metering Pump
1 metering pump + 1 GV= 1 motor + 1 syringe
New
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Application of Metering Pump
LEO(II)
Diff Bath
LEO(I)
Preheat 5
LBA
Preheat 7
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Application of Metering Pump
WC2-2 WC2-1
HGB Bath
Flow Cell
FCM
Diff & Baso
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Application of Metering Pump
SRV
DIL
DIL
New SRV
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Application of Metering Pump
SRV DIL
New
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Application of Metering Pump
DP1 DP2, 3 DP4 DP5,6 DP7 DP8
Specification 0.32mL 0.52mL 1.5mL 1mL 2mL 2.6mL
DIFF DIFF DIFF/BASO HGB HGB RBC
Usage
LEO(II) LEO(I) sample LH Diluent Diluent
BASO RBC
LBA Premixing
PS: DP2 & DP3, DP5 & DP6 are using different membrane inside, so could not exchange the position with
each other.
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Notice ： when using metering pump
Liquid port should be toward up in case of some air bubble remain in t
he pump.
Liquid port
Gas port
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Sampling Parts
120ul Blood (WB)
Sample Probe
2.5ml Syringe SRV 120ul Dilution (PD)
Pierce Needle 180ul Blood (WB)
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Sample Probe
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Pierce Needle
Sample aspirate
internal needle clean (Gas hole)
external needle clean Blow-dry
Sample aspirate Clean procedure (Sample hole & Gas hole)
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Syringe
Syringe
Syringe motor Syringe sensor
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Application of Syringe
FCM
Flow
l
Cel
BASO&DIFF
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Application of Syringe
Sample Aspiration
SRV with Diluent Cleaning
DIL
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Shear Valve
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Shear Valve
Assembly Chart
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Manufacture of SRV
Materials: special alumina ceramic
Advantage:
1. wearable, stability of chemical character, anti-rust, good temper
ature character
2. dispensing fast
Disadvantage: frangible, hard to machining
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Shear Valve Components
Inside
New
Sampling valve Rotary valve Rear fixed valve
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Shear Valve Components
Difference
BC5500 SRV, BC5800 SRV,
DIFF&BASO distribution hole=1*20ul DIFF&BASO distribution hole=2*10ul
RBC distribution hole=8.3ul RBC distribution hole=6ul
One position fixing hole Two position fixing hole: UP & DOWN position
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Shear Valve Components
Sketch Map
Sampling valve Rotary valve Rear fixed valve
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Function of SRV
Use flow paths, take the certain length and section to get or measure
the sample
Ration and shear the blood sample
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Function of SRV
SRV New
DP1
DP2
DP3
DP8
DP8 DP6
DP7
DP5
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Tubing Connection of SRV
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Work Principle of SRV (1)
Open-vial mode
The handle on the
sampling valve is
turned to the upper
position. 2.5ml syringe
aspirates sample into
the shear valve.
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Work Principle of SRV (2)
Open-vial mode
The handle of the sampling
valve is turned to the lower
position. The sampling valve
divides the sample into 4
segments and sends them
into different baths for
analysis.
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Work Principle of SRV (3)
Open-vial mode
The handle of the
sampling valve is on the
lower position. Certain
amount of primarily
diluted sample is
aspirated from the
premix bath.
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Work Principle of SRV (4)
Open-vial mode
The handle of the
sampling valve is turned to
the upper position. Certain
amount of primarily
diluted RBC sample is
injected into the RBC bath.
38 © 2011 Mindray Confidential
Work Principle of SRV (1)
Autoloader/close tube mode
The handle on the
sampling valve is
turned to the lower
position. 2.5ml syringe
aspirates sample into
the shear valve.
39 © 2011 Mindray Confidential
Work Principle of SRV (2)
Autoloader/close tube mode
The handle of the sampling valve
is turned to the upper position.
The sampling valve divides
the sample into 4 segments
and sends them into different
baths for analysis.
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Work Principle of SRV (3)
Autoloader/close tube mode
The handle of the sampling
valve is on the upper
position. Certain amount of
primarily diluted sample is
aspirated from the premix
bath.
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Work Principle of SRV (4)
Autoloader/close tube mode
The handle of the sampling
valve is turned to the lower
position. Certain amount of
primarily diluted RBC
sample is injected into the
RBC bath.
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RBC Pre-mixing Bath & RBC/PLT Bath
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Application of RBC Pre-mixing Bath
RBC Sample In
First Dilution
Out
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Application of RBC/PLT Bath
-0.04Pa
vacuum
New
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Volumetric Metering
This analyzer uses a volumetric metering unit to control the coun
t cycle and to ensure that a precise volume of sample is analyzed.
Uppersensor Uppersensor
Lowersensor Lowersensor
1Em
ptywhenstart 2Liquidsurfacefaldownthroughthe
volum
eritctube
Uppersensor Uppersensor
Lowersensor Lowersensor
3Countingstartwhenthe 4Countingendwhentheliquid
liquidsurfacepasstheupper surfacepassthelowersensor
sensor.
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HGB Bath
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Application of HGB Bath
Lyse HGB sample from SRV
Bubble mixing
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Pre-heat Bath
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Pre-heat Bath
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Application of Pre-heat Bath
SRV-Diff
LEO(I)
LBA
DIL
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WBC Reaction Bath
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WBC Reaction Bath
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Application of WBC Reaction Bath
FCM
LEO1
LBA
LEO2
Flow Cell
WC1
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Flow Cell Bath
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Application of Flow Cell Bath
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Application of FCM filter
New
Function:
Filter impurity of FCM, protect flow cell
Shelf life ： 2 year
Attention:
In the installation, inlet port must be upward
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Reagent, Waste and Buffer Chamber
DIL FCM
WC2
New
Buffer bath
Cleanser
WC1
WC3
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Reagent Chamber
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Waste and Buffer Chamber
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Application of DIL&FCM Chamber
Cleanser Container
DIL Container
Soak the liquid path Generate the sheath Sample dilute and cleaning
when shut down fluid in flow cell
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Application of WC1
WC3
Waste Container
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Application of WC2
(ASCO Valve Protection)
WC1
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Application of WC3
WC1
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FAQ1
What are the 8 metering pumps used for?
How can we maintain the liquid components?
Metering pump, Solenoid valve
Shear valve
DIFF/BASO bath, RBC bath, HGB bath
Flow cell
Reagent chamber
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FAQ2: How to Check…?
Sample volume
Metering pump
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FAQ3: How to Check…?
Premix/RBC/HGB bath/DIL/FCM chamber
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Counting Principle
© 2011 Mindray Confidential
Counting Principle
WBC channel
First count Diff channel, get
Lym%, Mon%, Eos%, Neu%
Then count Baso channel, get
WBC, Baso#, Baso%
Then get
Lym#, Mon#, Eos#, Neu#
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Counting Principle
HGB channel
Colorimetry
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Counting Principle
RBC/PLT channel
Electrical Impedance Method
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Counting Sequence
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Counting Sequence
Flow Chart of Whole Blood Mode
New
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Counting Sequence
Flow Chart of Prediluted Mo
de
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Liquid Diagram
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0~11 seconds
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Aspirate sample, test HGB blank, clean & drain (0-11s)
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11~18 seconds
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Dispense sample & lyse, aspirate from pre-mix bath, clean & drain RBC bath
(11-18s)
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18~23 seconds
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Dispense into RBC bath, prepare DIFF dilution & Sheath fluid, HGB dilution stay s
till(18-23s)
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23~34 seconds
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RBC/PLT, DIFF counting, clean pre-mix bath, diff bath, sample probe & wipe block
(23-34s)
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34~39 seconds
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RBC/PLT, HGB counting, WBC channel cleaning (34-39s)
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39~45 seconds
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Prepare Baso& Sheath fluid, clean RBC/PLT, HGB bath, prefill pre-mix, HGB bath; ready for next samp
le (39-45s)
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45~49 seconds
Clean the WBC counting module
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Clean RBC rear bath, prefill HGB, DIFF, BASO bath (4
5-49s)
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49~60 seconds
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Cleaning WBC channel, zap and prefill RBC bath, waste draining
(49-60s)
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Maintenance Sequence
© 2011 Mindray Confidential
Cleanser Cleaning
Cleanser Prime ：
Open SV13 and corresponding valves, prime cleanser by +70kPa pressure.
Cleanser Cleaning ：
1. Open SV17, use diluent cleaning corresponding tubings by +70kPa press
ure.
2. Use Metering pump and syringe to clean counting tubing again.
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Cleanser Maintenance
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Probe Cleanser Cleaning
It includes SRV, WBC bath, RBC bath, Flow Cell, HGB bath, Premix bath,
HGB bath maintenance.
Aspiration:
Overall Maintenance: Open SV07, PV10, SV11, SV16 and SV10, use DP4
aspirate 6ml (4 times) probe cleanser from Open-Vial probe into WBC c
hannel
and flow cell.
SRV Maintenance: Use 2.5ml syringe to aspirate probe cleanser into SR
V.
Dispensing:
Use 2.5ml syringe dispense probe cleanser into HGB, RBC, Pre-mix bath.
95
Cleaning: it is similar to cleanser cleaning.
© 2011 Mindray Confidential
Probe Cleanser Maintenance
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Troubleshooting
© 2011 Mindray Confidential
Abnormal PLT Background
PLT: 10~40. It is caused by dirty diluent, SRV or tubing.
Cleaning by background test, probe cleanser soaking and disassemble
cleaning.
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Abnormal PLT Background
PLT: If there is interference, usually there will be a peak wave bet
ween 0-10 FL.
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Abnormal PLT Background
Check the shielding cover of RBC bath and the signal processing boar
d whether they are installed well.
Check or decrease the RBC Gain. After that, you may recalibrate RBC,
PLT and MCV.
Check there is leakage in the RBC rear bath.
Check the isolation chamber under the RBC bath whether it is dirty.
100 © 2011 Mindray Confidential
RBC Bubble
RBC bath not enough diluent?
RBC rear bath leaking?
Anywhere leaking from rear bath to WC3?
Tube connectors loose?
RBC reference count time setting?
Volumetric sensors work well?
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No WBC Result (about 0.00)
Leucopenia: BASO channel blocked
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No WBC Result (about 0.00)
Check the reagent of LBA. Check the liquid parts adding LBA. Such as m
etering pump DP3, gas valve GV72, liquid valve SV27 and tubing from LB
A container to SRV.
Check the metal connectors of WBC reaction bath whether they are block
ed; Clean the SRV and the tubing between the SRV and WBC reaction Bath
.
Check the pinch valve PV12 and gas valve GV50.
Check the pressure 0.25MPa, 0.07MPa and -0.085MPa.
Check the signal processing board.
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Flow cell blocked
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Flow cell inner-surface dirty
Facula before SS PD
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Flow cell out-surface dirty
Direct light blocker
Out-surface dirty
Facula before SS PD
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Hemolysis insufficient
Lyse reagents, lyse dispense volume, temperature
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Lyse Resist (hemolysis insufficient)
Lyse resist & low LAS Gain
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Lyse Resist (hemolysis insufficient)
Check the ambient temperature; it should be much more than 15 centigra
de.
Check the Gain.
Check the temperature of WBC reaction bath and pre-mix bath.
Check the lyse LEO(I) and LEO(II); maybe themselves.
Check the tubing adding LEO(I) and LEO(II) and metal connectors of WBC
reaction bath;
Clean the SRV and the tubing between the SRV and WBC reaction Bath.
Checking the metering pump DP1 and DP2 whether the dispensing volume i
s enough. Also the gas valves (such as GV69, 71) and liquid valves (su
ch as SV25, SV26)
Check the pressure 0.07MPa and -0.085MPa.
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HGB Error or HGB Results Abnormal
Check and adjust HGB Gain to make sure the HGB blank voltage is 2.0-2.
4V (2.28V recommended).
Check the HGB bath whether it is dirty or there is leakage.
Check the HGB unit whether it is loose or dirty.
Check whether there is enough diluent dispensed into the HGB bath when
counting. If not, check GV73, and SV19.
Check whether there is enough LH lyse dispensed into the HGB bath when
counting. If not, check GV70, and SV28.
Observe the mixing to see if bubble is too big or none. If yes, check
GV65 and CV6.
Check the pressure 0.07MPa and -0.085MPa.
110 © 2011 Mindray Confidential
All the stars on the scattergrams
Check the sheath fluid in the FCM chamber whether it is enough. If not
, perform “Prime > FCM cistern” in the “Service > Maintain” screen
.
Check the corresponding liquid parts aspirating or dispensing the dilu
ent; such as SMC valve SV53, liquid valve GV80, tube kinkled or leaked
? Make sure no air going into FCM channel.
Check the liquid parts dispensing the sheath fluid (diluent) into the
flow cell; such as SV3.
111 © 2011 Mindray Confidential
Version Information
Version Author Date Description
1.0 Eric cheng 2012.6.18 BC-5800 Liquid System
112 © 2011 Mindray Confidential
Check Information
Version Reviewer Date Comments
1.0 Carl 2012.6.20 Initial Release
113 © 2011 Mindray Confidential
Thank you!
114 © 2011 Mindray Confidential
