Professional Documents
Culture Documents
Respiration Chapter
Video 3
Done by
DR.DADI SINDHU
Cellular Respiration
• Some yeast is added to the glucose solution and fixes two holed rubber
stopper to the flask.
• Oxygen supply from the air is cut off by pouring a layer of liquid paraffin on
the mixture(thermos is heat resistant can’t be used for
boiling
. Insert one end of thermometer into the thermos flask ,see the
end of thermometer kept in the solution.
. Gas released is collected in a tube containing bicarbonate solution or
lime water as shown in apparatus set up,keep the apparatus undisturbed
for one or two days.
Diagram showing the experiment on the
anaerobic respiration
OBSERVATION AND RESULT
• 1.After two days it is observed that lime water turns into milky
white precipitate.
• 2.Increase in temperature noted on yeast cells indicate that they
respire and release energy.
• 3.The smell of alcohol comes from the flask.
• RESULT:
• These observations indicate that yeast cells respire anaerobically
and release carbondioxide.
FERMENTATION
Temperature is not allowed to rise and controlled The temperature during combustion is very high.
otherwise it can damage the cell
CONTINUATION…..
RESIRATION COMBUSTION
Adenosine triphosphate is formed Adenosine triphosphate is not formed.
during this process. On breakage of
the molecular bond, energy is
released for physiological processes.
Oxidation occurs at the end of the Substrates are directly oxidized in the combustion process
reaction between oxygen and
reduced coenzymes in the electron
transport chain
Enzymes are required for the process Enzymes are not required for combustion as it is a non-cellular process
of respiration
Example: Aerobic respiration Example: Combustion of oxygen and hydrogen into water vapour