BIOL 1110 Module 4

Tutorial
Q1
What of the following statements about Taq polymerase are correct?
I. Taq polymerase is isolated from Thermus aquaticus, which is an
archaebacterium.
II. Taq polymerase can function at high temperature.
III. Taq polymerase can function at extremely salty condition.
IV. Mg2+ ions boost the activity of Taq polymerase.

A) I and II
B) I and III
C) II and III
D) II and IV
E) I, II and IV
Q2
Which of the following about symbiotic relationship is
correct?

A) All normal microbiota in a human body are in mutualistic relationship

with the human being.
B) In the symbiotic theory, the nucleus of eukaryotic cells is originated
by engulfment of a prokaryotic cell.
C) When a microorganism utilizes the waste product of another
microorganism, the two microorganisms are in mutualism.
D) Clownfish get food from anemone, so clownfish and anemone are in
commensalism.
E) Barnacles obtains food debris from the crab, who has not benefited
nor harmed, barnacles and the crab are in commensalism.
Q3
Which of the following about microorganism used in
industry is incorrect?

A) Indigo in blue jeans can be synthesized by bacteria.

B) Bt is a xenobiotic synthesized to kill pests.
C) Stone-washing effect in jeans can be performed by cellulase enzyme
extracted from a fungus.
D) Bt toxin gene can be inserted to the plant genome to make them pest
resistant.
E) Bt can be sprayed as a powder.
Q4
Which of the following about fermentation is correct?

A) It produces more ATP than aerobic respiration.

B) Carbon dioxide and lactic acid are the only two end-products
produced.
C) Cheese and yoghurt are produced by fermentation of milk.
D) L-amino acids are produced by fermentation of Saccharomyces
cerevisiae during beer production.
E) The key ATP production step in fermentation is Krebs cycle.
Q5
Which of the following statements about
biotechnology are correct?
I. All human proteins can be expressed and purified using
recombinant bacteria.
II. Botox is both a food poison and a medical therapy.
III. Yeast artificial chromosome (YAC) can be a DNA vector.
IV. Selectable marker is usually put at the multiple cloning site.

A) I and II
B) II and III
C) I, II and III
D) II, III and IV
E) All of the above.
Q6
Which of the following statement about SNP and STR
detection is correct?

A) Single nucleotide polymorphism (SNP) always occurs in intergenic

region.
B) Single nucleotide polymorphism (SNP) can always predict disease
risk.
C) The size of short tandem repeats (STR) can be detected by PCR, but
SNP cannot be detected by PCR.
D) The size of short tandem repeats (STR) can be different in the two
alleles.
E) Short tandem repeats (STR) always occur in coding regions.
Q7
Which of the following statements about transmission of
infectious diseases are correct?
I. Aerosols are smaller than mucus droplet and thus can travel farther.
II. Mosquitos can be fomite for transmission of pathogens.
III. Indirect contact transmission involves a non-living fomite.
IV. Vertical direct contact transmission refers to transmission from mother to
child through placenta or breastfeeding.

A) I and II
B) I and III
C) III and IV
D) I, III and IV
E) All of the above.
Q8
Which of the following about pathogens is correct?

A) Endotoxins are proteins produced inside pathogenic bacteria.

B) Exotoxins are the lipid portions of lipopolysaccharides that are part of
the outer membrane of the cell wall of bacteria.
C) Only endotoxins, but not exotoxins, cause damage to host cells.
D) Biofilm are microbe community that come together in masses, cling
to surfaces.
E) Salmonella typhi cause all typhoid fever’s sign and symptoms when
the bacteria are rubbed on skin.