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1. Which is the most abundant genetic material in the world?

A. DNA

B. RNA

C. Proteins

D. Both a and b

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2. Mark the incorrect match :

A. Bacteriophage lambda – 38502 base pairs

B. DNA – Two polynucleotide chains

C. Pitch of the helix of DNA – 3.4 nm

D. Distance between two consecutive base pairs in DNA – 0.34nm

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3. Which of the following is common in both DNA and RNA :

A. Thymine

B. Additional – OH group at 2’ position in the Ribose

C. Cytosine

D. Deoxyribose sugar

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4. Statement 1: There are two types of Nitrogenous bases –


Purines and Pyrimidines.
Statement 2: Uracil and Thymine are common in both DNA and
RNA.

A. Both Statement 1 and 2 are Correct

B. Both Statement 1 and 2 are Incorrect

C. Statement 1 is Correct

D. Statement 2 is Correct
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5. In a DNA strand the nucleotides are linked together by :

A. Hydrogen bonds

B. Glycosidic bonds

C. Peptide bonds

D. Phosphodiester bonds

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6. What is present at 3’ end of the Polynucleotide chain?

A. Phosphate group

B. Ribose sugar

C. Free -OH group

D. Purines

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7. ‘ 5- methyl uracil ‘ is another name for :

A. Cytosine

B. Thiamine

C. Thymine

D. Adenine

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8. DNA as an acidic substance present in the nucleus was first


identified by :

A. Frederick Griffith

B. Watson and Crick

C. Wilkins and Franklin

D. Friedrich Meischer

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9. Chargaff’s rules are applicable to :

A. Double stranded DNA

B. Double stranded RNA

C. Single stranded DNA

D. Single stranded RNA

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10.Statement 1: The bases in two strands of DNA are paired


through Phosphodiester bonds.
Statement 2: The two chains of DNA are coiled in a right – handed
fashion.

A. Both Statement 1 and 2 are Correct

B. Both Statement 1 and 2 are Incorrect

C. Statement 1 is Correct

D. Statement 2 is Correct
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11. The Central dogma in molecular biology, was proposed by :

A. Watson and Crick

B. Francis Crick

C. Frederick Griffith

D. Frederick Sanger

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12.Statement 1 : The DNA in nucleoid is organised in large loops


held by proteins.
Statement 2 : In a DNA helix, the distance between two
consecutive base pairs is 0.34 nm.

A. Both Statement 1 and 2 are Correct

B. Both Statement 1 and 2 are Incorrect

C. Statement 1 is Correct

D. Statement 2 is Correct
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13.Statement 1 : Histones are rich in Acidic amino acid residues


lysine and arginine.
Statement 2 : In Prokaryotes, Histones are organises to form a unit
of eight molecules called histone octamer.

A. Both Statement 1 and 2 are Correct

B. Both Statement 1 and 2 are Incorrect

C. Statement 1 is Correct

D. Statement 2 is Correct
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14.Statement 1: The negatively charged DNA is wrapped around


the positively charged histone octamer to form a structure
called Chromosome.
Statement 2: A typical Chromosome contains 200 bp of DNA helix.

A. Both Statement 1 and 2 are Correct

B. Both Statement 1 and 2 are Incorrect

C. Statement 1 is Correct

D. Statement 2 is Correct
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15.Thread – like stained (coloured) bodies seen in nucleus are


called as :

A. Nucleosome

B. Chromatin

C. Chromosome

D. Histone octamer

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16.Mark the incorrect statement :

A. Euchromatin is transcriptionally less active than


Heterochromatin

B. Frederick Griffith worked with Streptococcus pneumoniae

C. Transforming Principle was given by Frederick Griffith

D. DNA is negatively charged molecule

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17.Mark the incorrect statement :

A. S strain bacteria in Griffith‘s Experiment were Virulent

B. R strain bacteria causes Pneumonia

C. Frederick Griffith experimented with Streptococcus pneumoniae

D. Griffith did not discover the Biochemical nature of Genetic


material

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18.Prior to the work by Avery, MacLeod and McCarty, the genetic


material was thought to be a :

A. RNA

B. DNA

C. Protein

D. Polysaccharide

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19.Mark the incorrect statement with respect to Avery, MacLeod


and McCarty’s Experiment :

A. They purified biochemicals from the heat killed R cells

B. They discovered that protein digesting enzymes did not affect


transformation

C. They concluded that DNA is the hereditary material

D. Prior to their work, the genetic material was thought to be a


Protein
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20.Assertion : DNA has evolved from RNA with chemical


modifications.
Reason : DNA is more reactive hence unstable.

A. Both Assertion & Reason are True & Reason is Correct


Explanation of Assertion

B. Both As. & Reason are True but Reason is not Correct
Explanation of Assertion

C. Assertion is True but Reason is False


D. Both Assertion and Reason are False
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21.The semi-conservative replication of DNA was given by :

A. Meselson and Stahi

B. Watson and Crick

C. Frederick Griffith

D. Hershey and Chase

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22.Hershey and Chase worked with :

A. Bacteriophages

B. Viroids

C. Bacteria

D. Prions

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23.In Hershey and Chase Experiment, viruses grown on


Radioactive sulfur contained :

A. Radioactive DNA

B. Radioactive Protein

C. Radioactive RNA

D. Radioactive Polysaccharide

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24.In Hershey and Chase Experiment, what finding led to the


conclusion that DNA is the genetic material :

A. The Presence of Radioactivity in the bacteria when 32 S was


used

B. The Presence of Radioactivity in the bacteria when 35 S was


used
C. The Presence of Radioactivity in the bacteria when 32 P was
used
D. The Presence of Radioactivity in the bacteria when 32 Cl was
used
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25.Which property among those listed below is not a criteria for a


molecule to act as a genetic material :

A. Generate its replica

B. Chemically and Structurally stable

C. Provide scope for slow changes

D. Destroy itself after every cycle

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26.Statement 1 : RNA is labile and easily degradable.


Statement 2 : RNA is more reactive than DNA .

A. Both Statement 1 and 2 are Correct

B. Both Statement 1 and 2 are Incorrect

C. Statement 1 is Correct

D. Statement 2 is Correct

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27.Assertion : RNA mutate at a faster rate.


Reason : RNA can directly code for the synthesis of proteins.

A. Both As. and Rs. are True and Rs. is Correct Explanation of As.

B. Both As. and Rs. are True but Rs. is not Correct Explanation of
As.

C. Assertion is True but Reason is False

D. Both Assertion and Reason are False

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28.Statement 1 : For the transmission of genetic information, RNA


is better.
Statement 2 : RNA being less stable is preferred for storage of
genetic information.

A. Both Statement 1 and 2 are Correct

B. Both Statement 1 and 2 are Incorrect

C. Statement 1 is Correct

D. Statement 2 is Correct
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29.Statement 1 : Essential life processes such as Metabolism,


Translation, Splicing provide evidence that RNA was the first
genetic material.
Statement 2 : DNA has evolved from RNA from chemical
modifications that make it more reactive.

A. Both Statement 1 and 2 are Correct

B. Both Statement 1 and 2 are Incorrect

C. Statement 1 is Correct
D. Statement 2 is Correct
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30.Which of the following organism was used to prove


Semiconservative DNA Replication :

A.Bacteriophage

B. E. coli

C. Streptococcus pneumoniae

D. Tobacco mosaic virus

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31.Which of the following compound was used for density gradient


centrifugation in Meselson and Stahi Experiment?

A. CsCI

B. NaCl

C. CaCl

D. KCl

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32.Which of the following was used by Taylor and Colleagues to


detect distribution of newly synthesised DNA in the
Chromosomes :

A. NH4Cl (where N was N15 isotope)

B. CsCl

C. Radioactive thymidine

D. Radioactive Sulfur

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33.Which of the following enzyme is essential for DNA replication?

A. DNA polymerase

B. DNA primase

C. RNA polymerase

D. All of the above

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34.Which of the following provide energy for polymerisation


reaction during DNA Replication?

A. Deoxyribonucleotide triphosphates

B. Deoxyribonucleoside triphosphates

C. Deoxyribonucleoside biphosphates

D. Deoxyribonucleoside monophosphates

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35.The DNA – dependent DNA polymerases catalyse


polymerisation in which direction :

A. 5’ -> 3’

B. 3’ -> 5’

C. Both a and b

D. None of the above

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36.The template DNA strand showing discontunous Replication


has Polarity in which direction :

A. 5’ -> 3’

B. 3’ -> 5’

C. 5’ -> 5’

D. 3’ -> 3’

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37.Statement 1 : DNA polymerases can initiate the process of DNA


Replication on their own.
Statement 2 : In Eukaryotes, DNA Replication takes place at S-
phase of the cell cycle.

A. Both Statement 1 and 2 are Correct

B. Both Statement 1 and 2 are Incorrect

C. Statement 1 is Correct

D. Statement 2 is Correct
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38.The process of copying genetic information from one strand of


the DNA into RNA is termed as Transformation.
The above statement is :

A.Correct

B.Incomplete

C. Incorrect

D. None of the above

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39.Assertion : Both DNA strands are not copied during


transcription.
Reason: If both strands act as a template, they would code for
RNA molecule with same sequence and this would complicate the
genetic information transfer machinery.

A. Both As. and Rs. are True and Rs. is Correct Explanation of As.

B. Both As. and Rs. are True but Rs. is not Correct Explanation of
As.
C. Assertion is True but Reason is False
D. Both Assertion and Reason are False
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40.With respect to the polarity of coding strand, what is the location


of promoter?

A. towards 5’ end of the Structural gene

B. Towards 3’ end of the Structural gene

C. Towards downstream of the structural gene

D. None of the above

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41.Monocistronic Structural gene is found mostly in :

A. Eukaryotes

B. Prokaryotes

C. Both a and b

D. Viruses

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42.Statement 1 : The Expressed sequences are defined as Introns.


Statement 2 : The Introns are interrupted by Exons.

A. Both Statement 1 and 2 are Correct

B. Both Statement 1 and 2 are Incorrect

C. Statement 1 is Correct

D. Statement 2 is Correct

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43.Which of the following RNA plays Structural and Catalytic role


during Translation?

A. mRNA

B. tRNA

C. rRNA

D. All of the above

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44.In which of the following organism transcription and translation


take place in the same compartment?

A. Bacteria

B. Eukaryotes

C. Both a and b

D. None of the above

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45.RNA polymerase III is not responsible for transcription of :

A. tRNA

B. 5srRNA

C. Both a and b

D. 18S RNA

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46.In which of the following process adenylate residues are added


at 3’ –end of hnRNA :

A. Capping

B. Tailing

C.Splicing

D.Slicing

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47.Which of the following is not a feature of genetic code :

A. The codon is triplet

B. The code is Universal

C. GUG has dual functions – It codes for Methionine and also act
as initiator codon

D. UAA, UAG, UGA are stop Terminator codons

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48.Statement 1 : There are seperate tRNAs for each STOP Codon.


Statement 2 : The tertiary structure of tRNA is like a clover – leaf.

A. Both Statement 1 and 2 are Correct

B. Both Statement 1 and 2 are Incorrect

C. Statement 1 is Correct

D. Statement 2 is Correct

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49.The cellular factory responsible for synthesising protein is called


:

A. Lysosome

B. Nucleolus

C. Ribosome

D. Mitochondria

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50.The Untranslated regions are present at :

A. 5’ end of mRNA

B. 3‘ – end of mRNA

C. Both a and b

D. None of the above

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51.Which of the following acts as Start codon:

A. UAG

B.GUG

C. UAA

D. AUG

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52.Statement 1 : The lac operon consists of one regulatory gene.


Statement 2 : The i gene codes for the repressor of the lac operon.

A. Both Statement 1 and 2 are Correct

B. Both Statement 1 and 2 are Incorrect

C. Statement 1 is Correct

D. Statement 2 is Correct

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53.Which of the following gene codes for an enzyme that increases


permeability of the cell to β- galactosides –

A. y gene

B. i gene

C. z gene

D. α gene

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54.Which of the following act as inducer in the lac operon :

A. mRNA

B. Lactose

C. i gene

D. Promoter

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55.Regulation of lac operon by repressor is refered to as :

A. Negative Regulation

B. Positive Regulation

C. Both a and b

D. None of the above

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Biology at Ease
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1) A Answers 41) A
2) A 21)B 42) B
3) C 22)A 43)C
4) C 23)B 44) A
5) D 24)C 45) D
6) C 25)D 46) B
7) C 26)A 47) C
8) D 27)B 48) B
9) A 28)C 49) C
10)D 29)C 50) C
11) B 30)B 51) D
12)A 31)A 52) A
13)B 32)C 53) A
14)B 33)A 54) B
15)B 34)B 55) A
16)A 35)A
17)B 36)A
18)C 37)D
19)A 38)C
20)C 39)C
40)A
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