SAMPLE PREPARATION TECHNIQUES IN

ANALYTICAL CHEMISTRY
GROUP 6

Development of a QuEChERS-based method

combined with gas chromatography
mass spectrometry for the analysis of
alkanes in sediments

Lecturer: PhD. Tran Thi Thanh Thuy

 MEMBER ASSIGNMENTS
MEMBERS ID TASKS

Pham Thi Thu Thao 20002021 Prepare Introduction, edit ppt

Le Tan Bao 20001931 Prepare Scope of application

Ho Ngoc Kieu 20005051 Prepare Meaning of method, present

Tran Thi Hong Phuc 20005541 Prepare Principle of method

Ho Hong Phuc 20001841 Prepare QuEChERS Extraction, present

Tran Anh Tu 20035401 Summarize contents, edit ppt

 CONTENTS
INTRODUCTION 1

2 APPLICATION

MEANING OF 3
METHOD
PRINCIPLE OF
4
METHOD
QuEChERS
5
EXTRACTION
 1
INTRODUCTION
 • Was introduced in 2002.
QuEChERS • A type of dispersive solid
phase extraction (dSPE)
Quick used for sample preparation.
Easy
✓Simple • Determine 29n – alkanes
Cheap (C12 – C40) and two
Effective
✓Effective isoprenoid acyclic
hydrocarbons (pristane and
Rugged
Safe ✓ High quality
results
phytane) in sediments.
 2
APPLICATION
 Extract alkanes from C22 to C34
with an odd to even predominance,
highest in C31. This is consistent
with a vascular land plant input.
These results can be used for
further paleoclimate investigations.

Lady-fern from Vascular plants

 3
MEANING OF
METHOD
 Develop a method:

✓ 💰 Low cost
✓ ⏳ Short analytical time
✓ possible associated contamination
Reduction of solvent consumption and

✓ Avoid the need for sophisticated devices

✓ Diminish possible auto-oxidation reactions
Alkane extraction by QuEChERS method would be more
 in line with the principles of Green Analytical Chemistry.
 4
PRINCIPLE OF
METHOD
 Partitioning dSPE clean up

ACN
LC-MS

• The analyte in the sample is extracted in a suitable solvent.

• The analyte in the extract is cleaned with an impurity
adsorption.
 5
QuEChERS
EXTRACTION
 SAMPLE SELECTION
 Samples were collected from archaeological sites in
Austria and Spain.
 Samples is described as homogeneous beige silt,
approximately 1.30 m below surface.
 Collected by metal tools
rinsed with MeOH and DCM
⇒ wrapped in aluminum foil
⇒ stored at -20°C.
Sample extraction
Clean up Extraction
 REFERENCE
Antonio V. Herrera-Herreraa, Naima Mohamed-
Rodrígueza, Bárbara Socas-Rodríguezb, Carolina Mallol.
Development of a QuEChERS-based method combined
with gas chromatography-mass spectrometry for the
analysis of alkanes in sediments