General Microbiology

AJ. TREE VATAKIT (M.S.,Food Technology)

Program in Agro-Industry
Nakhon Phanom College of Agriculture and Technology
Nakhon Phanom University
http://agri.npu.ac.th

General Microbiology

-
-
-

(pure culture)


1. (streak plate)

1.1 simple streak

(loop)

Simple Streak

1.2 cross streak

simple streak
3-4
loop (cross)

2448 .

Cross streak plate

 10 (Ten fold serial dilution)

30-300

Diluient

Nutrient Broth, 0.85 % NaCl, Peptone water


2.1 Plate Count

(clump)

2 pour plate spread plate


2.2 Spread Plate

( Serial dilution )
aseptic technique
0.1 ml.
Sterile spreader L
colony
Spread plate Pour
plate

 spread plate
1. 10 1:
104
0.1 ml. nutrient agar
9 ml.
5
1:105
2. 1: 105
0.1 ml. nutrient agar
9 ml.
5
1 :106

 spread plate
3. 1 :106
0.1 ml. nutrient agar
4. Sterile spreader L
nutrient agar 37
C 24-48
5. , colony
colony nutrient agar colony
( colony counter )


2.3 Pour Plate

Serial dilution
Pour plate

colony

, colony
colony
psychrophiles mesophiles

 Pour Plate

 Pour Plate
3.1. 1 :103 1:104 1 : 105
1 ml. 2
1 dilution
1 :103 1:104 1:104 1 : 105
1 : 105
3.2.
water bath

 Pour Plate
3.3.

3.4.

3.5. 37 C 1-2
colony colony
colony colony
Streak plate

2.4 Membrane Filtration

0.45

 Membrane Filtration


2.5 Most Probable Number (MPN)


Escherichia coli
3
10 10-1 10-2 10-3 3
(duhrum tube)



MPN

5 3
MPN

 E.coil MPN 5
5-2-0 MPN
5 MPN 4.9 MPN/ml
10 -1 10-2 10-3
MPN 0.49 MPN/ml of E.coli

MPN table

CFU
Aseptic technique
Pour Plate
Spread plate

MPN


.2544. . 3
. .42-73
. 317 211 []
202.28.94.202/.../317_211_-_Bacteria_(1)_2550_2-small.pdf [ 2553]
Adams, M. R. Food microbiology. / M. R. Adams and M. O. Moss. Cambridge :
Royal Society of Chemistry, 1995.
Atlas, Ronald M. Principles of microbiology. / Ronald M. Atlas. Boston, Mass. :
WCB/McGraw-Hill, 1997.
Bacteria. [On-line] Retrieved from www.jochemnet.de/fiu/lab7.pdf [June 2010]