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H CH MINH
TRNG I HC BCH KHOA
KHOA K THUT HA HC
...............0 CO 03..............................
ti:
HEMOGLOBIN V MYOGLOBIN
60800300
60802379
60800288
60800272
60802571
T THANH LOAN
Mc Lc
60801123
HEMOGLOBIN V MYOGLOBIN......................................1
Chng I: Hemoglobin...........................................................................................................................................3
Oxy ho............................................23
Metmyoglobin Fe3+(mu nu)............................23
CC BIN I CHNH CA MYOGLOBIN TRONG THT (GD - globn
b bii tnh)........................................25
Chng I: Hemoglobin
1.1 Cu to :
Hemoglobin l huyt cu t ( Hb) mt cromoprotein c mu trong hng cu ng vt bc
cao.
Hemoglobin cu to gm hai phn :
phn protein thun : globin phn
nhm ngoi hem
Heme
<xchan
2
Heme
1.1.1 Hem :
Hemoglobin molecule
heme
protoporphyrin IX
Hs
CH =CHj
Ferrochel atase
CH22
CH
I.
COO
COO
'CO
'
O'
1.1.2 Globin :
CH
H3C
CH = CH 2
H3C
CH3
COOH
COOH
"Shortiand" cx-helix
cu trc bc 2 v bc 3 ca hemoglobin
cu trc bc 2 v bc 3 ca hemoglobin
Garrett &
Grisham:
Biochemistry,
Figure 15.24
2/e
* S tng
hp globin
Transferrin
Saunders College
Publishing
Hnh 7:
Tng hp Hemoglobin
-2
(*1
5'
Chromosome 11 Chromosome 16
LCR I HS-40 5' m
HEMOGLOBIN V MYOGLOBIN........................1
Chng I: Hemoglobin...................................................................................................3
Oxy ho...............................23
Metmyoglobin Fe3+(mu nu)..............23
CC BIN I CHNH CA MYOGLOBIN TRONG
THT (GD - globn b bii tnh)........25
Embryo
n u n u nU^.
& Ok L
h
"p
22
F
p
"
a2 F
A2
1
2
.A
Fetus
Adult
tu xng
Tiii non
Cu trc
%
Hb A
A2b2
95-97%
Hb A2
A2d2
2-3.5%
Hb F
A2g2
1-2%
0=0
H IS (F8)
HIS
(F 8 )
Phn t Hb hon chnh l mt protein hnh cu c ng knh khong 55A phn t lng
64454.Hb gm 4 n v ging nhau tng i mt lin kt vi nhau bng lin kt phi ng
ha tr, mi bn n v gm mt chui polypeptide ca globin lin kt vi mt hcm l v tr
nh v gn oxy.T l st trong hemoglobin l 0,34%
M
1.2
Tnh
cht ca
hemoglobin :
1.2.1 Kt hp thun nghch vi oxy to oxyhemoglobin
Hb 1+
* 2 = Hb02
thm
ti
S kt hp v phn ly gia 02 v Hb c xc nh bi phn p oxygen mi trng
chung quanh Hb.
phi: phn p 02 cao 95-98% Hb kt hp oxy theo mu n m.
m: phn p 02 thp (40mmHg) phn ng xy ra theo chiu nghch, Hb02 phn
ly, nh 02 cho m
chc nng ca Hb trong h hp.
ng cong phn ly oxy -hemoglobin:
S kt hp v phn ly gia 02 v Hb c xc nh bi phn p oxy (p02)
C dng Sigma
Vic gn oxy vo hem th nht lm d dng gan oxy vo hem th 2
ng cong phn ly
oxy hemoglobin
* phn P02 thp (ni Hb02 nh 02 cho m): ng cong rt dc 1 s thay i nh ca P02
s lm thay i s giao 02 cho m mt cch ng k => gip hng nh P02/m.
* phn P02 cao (ni Hb ly 02 t phi) ng cong t:
P02: 60mmHg bo ha Hb vi 02: 89%.
P02 t 500 th bo ha Hb02 cng ch 100%
> P02 mi trng, ph nang c th thay i nhiu nhng bo ha Hb02 & lng oxy giao
m khng b giao ng lm.
=> P02 m on nh.
=> Hb c tnh m gip P02/ m n nh, m bo s hng nh ni mi.
V tr bnh thn ca ng cong ty thuc vo:
S Nng 2,3-DPG s Nng ion H+ (pH)
S C02 trong hng cu s Cu trc ca Hb...
2,3-DPG cao
H+ cao
C02 cao
Hb
Oxyhaemoqlobin____________Deoxhaemoglobin
2,3-DPG thp
Val FG5
Leu FG3
Leu F4
0.6 A
Heme
-s
_ i _ _ His F8
Porphyri
n
Porphyrin I
Helix F
Leu F7
(b)
(a)
15
H2O
CA
+ CO2T
MetHb + e'
(Fe )
nitrit, clorat,
ferricyanua...
I.2.5.
Tnh cht enzym:
Hb c tnh cht ca mt oxydoreductase xc tc phn ng oxi ha kh s
Tnh cht ca mt peroxydase:
H2O2 + AH2
2H2O +
A
Hb
s Tnh cht ca mt catalase:
H2O2
2H2O + O2
16
HLs E7
His E7
N'
HC-.
CH
CH
-N
HC.
N'
/
TV
His F8
HLs F8
N
(a) Free heme
with imidazole
N
(b) Mb:CO complex
(c)
Oxymyoglobin
Saunders College
Mt Hb c th gn 4 phn t 02.
Publishing
18
Helix Boundaries A1
of THE a AND
A16B1
C HAINS
of H UMAN
B16
Cl
H EMOGLOBIN
C7
AND OF
D1
H UMAN M YOGLOBIN 4 -*
D7
El
HEMOGLOBIN V MYOGLOBIN.......................................1
Chng I: Hemoglobin................................................................................................................................................3
Oxy ho............................................23
Metmyoglobin Fe3+(mu nu).............................23
CC BIN I CHNH CA MYOGLOBIN TRONG THT (GD - globn
b bii tnh).........................................25
Mb...............................G - L S D G E W Q L V L N V W G K V E A D I P G H G Q E V L I R L F K ^ H P E T L E K F D K F K H L K S E D E M K A S E D L K K H G A T V L T A L G
FI
F9
G1
'The first and last residues in helices A-H are indicated, whereas the
residues between helices constitute the intervening segments. The refined
Hb structure reveals that much of what is designated the EF segment is
really helical in both chains: It encompasses residues EF4-F2 and is designated the F' helix.
G19 HI
H19
H21
H26
HEMOGLOBIN V MYOGLOBIN....................................1
Chng I: Hemoglobin.......................................................................................................................................3
Oxy ho...........................................23
Metmyoglobin Fe3+(mu nu)..........................23
CC BIN I CHNH CA MYOGLOBIN TRONG THT (GD globn b bii tnh)...............................25
19
Mb........G I L K K K G H H E A E i K P | a Q S | T K H K I P V K Y L E F I S E C I I Q V L Q S K H P G D F G A D A Q G A M N K A L E L F R K D M A S N
20
1. hnh thnh lin kt hydro vi cc phn na dioxygen, tng o 2 rng buc khng i
2. ngn chn s rng buc ca kh carbon monoxide, cho d c hoc khng c t bn trong c th.
Carbon monoxide lin kt vi st trong mt ngy cui thi trang, v l cn tr bi s hin din ca
cc histidine ngoi bin, m lc lng n thnh mt conformation cong, co lin kt vi heme 23.000
ln tt hn so vi
o 2 nhng ch c 200 ln tt hn trong hemoglobin v myoglobin. Oxy gn trong mt thi trang b
cong, m c th ph hp vi histidine ngoi bin. [l2]
3. e ngn chn dimerization khng th o ngc ca oxymyoglobin vi mt loi
deoxymyoglobin
21
heme plane
11.2 Chc
nng
ca
chnh
chuyn
ch
mitochondria)
Xc tc vt vc NO
(p rotein chain)^/^!
dng
H
22
D esoxymyoglobin
Fe2+( ta)
Oxymyoglobin
phn ng cng oxy
Fe2+( ti)
Oxy ho
Oxy ho
Kh
Kh
Metmyoglobin
Fe3+(mu nu)
V
Desoxymioglobin l sc t bm sinh ca tht lm cho tht c mu ta, trong Fe c
ha tr II.
Myoglobin (Mb) khi mang oxy to thnh Oxymyoglobin (Mb02) c mu rc. Mb02
l cht d tr oxy cho c, trong Fe c ha tr II. Mu ca Mb02 c th nhn thy r
trn b mt tht ti.
Mb v Mb02 khi b oxy ha th Fe2+ chuyn thnh Fe3+ to nn Metmyoglobin (MMb)
c mu nu. Khi nguyn t Fe trng thi Fe3+ th phn t Mb khng c kh nng kt
hp vi oxy na.
23
ferrihemochrom
Nitrosomyoglobin
Nitrit
Nhit
Fe2-, Globin
hoc
nitrat
kh
iu kin
Fe3+, Globin
bin tnh,mu
nn
+
nhit
Oxy
mn (n
Ferrohemochro
Nitrosoferrohemochrom
ha
2+
MbdCO (Fe2+,GD)
Phc khng mu
Quang
MbdNO(Fe^,GD)
N ltr oz ofer oh emo crom
Pocphirin r do, b
oxy ho
Cho lemy o g
(nu,vng, khng
lob in (xanh l
cy)
a.theo loi
Loi
Lng
Myoglobin(mg/g)
Mu
Tht heo
Hng
Tht cu
nht
Tht b
anh o
b.thco loi c
Loi CO'
Lng mvoglobin
C vn ng
12 mg/g
C tr
6mg/g
26
c.theo tui
Loi
(theo tui)
Lng
myoglobin(mg/g)
Tht b
Tht b (non)
Tht b (non)
Tht b (gi)
18
Ti liu tham kho : Ha sinh cng nghip . L Ngc T ( ch bin ) Nh xut bn khoa hc k
thut H Ni
Ha sinh y dc
27