B CNG THNG

TRNG I HC CNG NGHIP THC PHM TP.HCM

KHOA CNG NGH THC PHM

BI TIU LUN K THUT THC PHM III

TI:

CNG NGH SN XUT

ENZYME PAPAIN

NHM SV THC HIN:

M SV:

1.NGUYN TH THY HNG

3005100260

2.NGUYN TH KIM TUYN

3005100886

3.NGUYN TH HNG VN

3005100905

4. TH HIP

3005100224

5.L TH NGH

3005100468

GVHD: TRN TH MINH H

TP.H Ch Minh, ngy thng nm ..

B CNG THNG
TRNG I HC CNG NGHIP THC PHM TP.HCM
KHOA CNG NGH THC PHM

*****

BI TIU LUN K THUT THC PHM III

TI:

CNG NGH SN XUT

ENZYME PAPAIN

Trnh by: Nguyn Th Thy Hng

Hnh nh: Nguyn Th Kim Tuyn

Nhm 4

Trang 1

MC LC
CHNG I : LI M U .................................................................................. .3
CHNG II: GII THIU V ENZYME PAPAIN ...................................................... 4
2.1Gii thiu v cy u .................................................................................. 4
2.1.1. Phn b sinh thi ca cy u ........................................................... 4
2.1.2.Hot tnh sinh hc v cng dng ca mt s cht c trong cy u ..... 5
2.2 Gii thiu v enzyme papain......................................................................... 6
2.2.1 Ngun gc ............................................................................................ 6
2.2.2 Tnh cht ca papain ............................................................................. 6
2.2.2.1 Tnh cht vt l ........................................................................... 6
2.2.2.2 Tnh cht ha hc ....................................................................... 7
a) Cu to ha hc: ............................................................................................ 7
b) Cu trc khng gian ....................................................................................... 8
c) Cu trc tm hot ng ca papain ................................................................ 8
d) Cc yu t nh hng n hot tnh xc tc ca papain ................................ 11
CHNG III: QUY TRNH CNG NGH SN XUT ENZYME PAPAIN ................... 13
3.1 Nguyn liu ................................................................................................ 13
3.2 Thit b . 13
3.3 Quy trnh cng ngh. 13
3.3.1 Thu nhn papain................. 15
3.3.2 S quy trnh cng ngh.. 16
3.4 ng dng ca enzyme papain 18
3.4.1 Trong y hc. 18
3.4.2 Trong cng ngh thc phm.. 18
3.4.3 Trong cng nghp khc . 18
CHNG IV: KT LUN 19
CHNG V: TI LIU THAM KHO. 20

Nhm 4

Trang 2

CHNG I :
LI M U
Trong m u c ba enzyme chnh bao
gm: papain, chymopapain v peptidase.
Papain l mt enzym c ly ra t m ca
cc qu u xanh. Ngoi ra, papain cn l
mt loi men phn gii protein tn ti trong
u . Enzym papain rt tt cho h tiu
ha, gip tiu ha cc thc n giu protein
mt cch d dng hn. N c th gip phn
gii v loi b nhng lp da cht trn b
mt c th. Papain c tc dng tiu ha
protid, bin i cc cht c albumin thnh pepton, c tc dng trn m, trn cc
hydrat carbon trong mi trng hi kim hay trung tnh. nhit thng khi
cho tip xc papain vi lng trng trng th lng trng trng b mt tnh snh
sn st. Trung bnh mt tri u cho c 12g m, t 4kg m u ti cho
1kg papain kh v trng u khai thc ly m ch hiu qu trong vng 3 - 4
nm tuy vng i ca mt cy u khong 20 nm.
Ngy nay, cng vi s pht trin mnh m ca Cng ngh sinh hc , cc ch
phm enzyme c sn xut ngy cng nhiu v c s dng hu ht trong cc
lnh vc nh: ch bin thc phm, nng nghip, chn nui, y t Khong 75%
ch phm l enzyme thy phn c s dng cho vic thy phn c cht t
nhin. Vi nhng li ch v gi tr m enzyme papain em li , n c ng
dng rng ri v thng c dng trong lnh vc ch bin m phm. Ngoi ra,
Papain rt cn cho nhiu lnh vc cng nghip: dc phm, ha cht, k ngh t
si dt may, thuc da, thc phm...V chnh l nguyn nhn a nhm em
n vi ti trnh by v cng ngh sn xut enzyme papain.
Nhm 4

Trang 3

CHNG II:
GII THIU V ENZYME PAPAIN
2.1 Gii thiu v cy u
Cy u cn c gi th Hu, phin mc, c lo, phin qua, phan
qua th, l hong phl (Campuchia), mc hung (Lo), m hng (Thi). u
thng l cy ng chu, nhng u c th xp thnh 3 loi trn phng din
gii tnh: cy c, cy lng tnh v cy ci. Vi cy u cng c th tr c ba
loi hoa ni trn. Ngoi ra cng c cy ra hoa khng hn hon ton c, ci hay
lng tnh m li pha ln nhiu t c tnh ca ba loi hoa. Khuynh hng thay
i gii tnh phn ln do thi tit gy ra t nh kh hn v thay i nhit .
Tn khoa hc: Carica papaya L., thuc h u - Caricac.

Hoa lng tnh

hoa ci

hoa c

2.1.1 Phn b sinh thi ca cy u .

Chi Carica L. c ngun gc vng nhit i chu M. vng ni cao
(1500-3000m), t Panama n Bolivia, cy u trng hin nay rt c th l
ging lai t nhin ca loi C. peltata Hook & Ann. Vo khong th k 16, ngi
Ty Ban Nha a u vo trng vng Carib v mt s nc ng Nam

Nhm 4

Trang 4

. T cc a im ny, cy tip tc c trng rng ri n , Srilanca,

chu i Dng v chu Phi.

Vn cy u
2.1.2 Hot tnh sinh hc v cng dng ca mt s cht c trong cy u
1. Nha u c th gy vim da.
2. Trung M, trong dn gian, ngi ta s dng u iu tr bnh
lyamip (Entamoeba histolytica), mt loi k sinh trng gy tiu chy dng l v
bin chng p- xe gan.
3. Samoa, ngi dn dng phn di v thn cy u cha chng
nhc rng.
4. Nha u c cha papain, l mt trong hai loi men tiu hy protein
(proteolytic enzymes) c tc dng lm mm tht bp. Chnh do tc dng ny, khi
dng u hm chung vi tht, tht s mm hn. Ngi dn vng Ca-ri-b,
Trung M cho bit h c th dng khu phn vi s lng ln tht c nhng vn
khng h g nu n u xanh sau .
5. Phn cm u l thnh phn chnh ca cc loi m phm nh kem nn
(mt), kem nh rng, x bng gi u.

Nhm 4

Trang 5

6. Cc ng dng quan trng trong y hc ca nha u l chit xut papain

dng trong phu thut ct sng (l mt loi "dao phu thut t nhin" m
a m). Nghin cu cho thy chit xut papain c hot tnh khng sinh
(antibioticactivity) vi tc dng chng vi khun gram dng (gram-positive
bacteria). N cn c dng iu tr l lot, lm tiu gi mc trong bnh
bch hu, chng kt dnh sau phu thut, lm thuc gip tiu ha. Trong cng
nghip, papain c dng tinh ch bia; x l len v la trc khi nhum; l
ph gia trong cng ngh ch bin cao su; khi tinh ch du gan c tuna, ngi ta
tim papain vo gan trc khi chit xut, lm cho thnh phm giu Vitamin A
v D hn. Khong 1,500 qu u xanh c va cho c khong 650g papain.

2.2 Gii thiu v enzyme papain

2.2.1 Ngun gc
Enzyme papain c tch ra t nha tri u xanh, thuc nhm enzyme
thy phn c ngun gc thc vt c nghin cu nhiu nht v tnh cht v c
ch hot ng. Papain l mt sulfhydryl protease, ln u tin c tch ra bi
Bals v cng s ca ng, sau bi Kimmel va Smith t nha kh. Trong nha
u ngoi enzyme papain cn c cc loi protease khc nh chymopapain,
caricain, glycyl endopeptidase v mt s enzyme khc (Baines and Brocklehurst, 1979). Nha u c hm lng v hot tnh papain cao nht tp trung
vng c nng nng v m n nh quanh nm.
2.2.2 Tnh cht ca papain
2.2.2.1 Tnh cht vt l
Bng 1: Tnh cht vt l ca papain
Tnh cht vt l

Gi tr

im ng in

pI = 8.75

Hng s sa lng S20

2.42 0.04

Hng s phn tn D20 (10-7giy.cm2)

10.27 0.13

Phn t lng

20,700

Nhm 4

Trang 6

 trin quang []D

-66.70

xon

17%

Vng hiu ng cotton

290nm

Th tch ring phn V(mL/g)

0.724

Tr s ma st f/fo

1.16

Bt mu vng hay mu nu nht, ty thuc phng php sy, khng tan trong
hu ht cc cht hu c nhng tan mt phn trong H2O hay glycerine. Bn
nhit.

2.2.2.2. Tnh cht ha hc

a) Cu to ha hc:
Papain l mt endoprotease c cha 16% N v 1.2% S. Papain l mt protease
thiol, theo nghin cu ca R. L. Hill v E. L Smith, papain l mt chui
polypeptide gm 185 amino acid, trng lng phn t l 20.900 Dalton.
Bng 2: Thnh phn ha hc:
Amino acid
Alanine
Arginine
Aspartic acid
Half Cysteine
Glutamid acid
Glycine
Histidine
Isoleucine
Leucine

S lng
Amino acid
13
Lyeine
10
Proline
17
Serine
6
Threonie
17
Tryptophan
23
Tyrosine
2
Valine
10
Methionine
10
Tng cng: 185

S lng
9
4
9
11
7
5
17
15
0

Theo kt qu phn tch bng tia X, phn t papain c cu to bi 212 acid

amin trong khng c cha methionine. Phn t lng khong 23,350 Da,
phn t l mt mch polypeptide vi u N l isoleucine, u C l asparagine,
c 6 gc cysteine to thnh 3 cu disulfur cc v tr 22-63, 56-95, 153-200

Nhm 4

Trang 7

khng c chc nng sinh hc, ch lm tng tnh bn vng ca cu trc v mt

nhm SH t do v tr 25.
b) Cu trc khng gian
Phn t papain c dng hnh cu vi kch thc 36x48x36Ao v mch chnh
b gp thnh hai phn ring bit bi mt khe. Trung tm hot ng nm ti b
mt ca khe ny, nhm -SH hot ng ca cysteine 25 nm bn tri khe v
nhm histidine 159 nm bn phi khe. Phn xon chim 20% ton b cc
amino acid c trong phn t.

Cu trc khng gian ca enzyme papain

Hot tnh ca papain da trn hai tm hot ng l Cys25 v His159.
Khong pH hot ng ca papain kh rng (3.5 8.0) ty thuc vo c cht.
Khi c cht l casein th hot tnh ti u ca papain trong vng pH t 5.7 7.0
v nhit thch hp l 50 570C.
c) Cu trc tm hot ng ca papain

Nhm 4

Trang 8

- Tm hot ng ca papain gm c nhm SH ca cysteine 25 v nitrogen

bc 3 ca histidine 159. Bn cnh nhm imidazole ca His 159 cng lin kt
vi Asp 175 bi lin kt hydrogen.
- Vng tm hot ng ca papain cha mch polypeptide vi cc amino acid
l:
Lys-Asp-Glu-Gly-Ser-Cys-Gly-Ser-Cys.
- Theo cc nghin cu ca Lowe, chui polypeptide trong trung tm hot
ng ca papain gn ging nh ca ficin hay trypsin, mc d chng c ngun
gc khc nhau.
Ficin: Arg-Glu-Glu-Gly-Glu-Cys-Gly-Ser-Cys.
Trypsin: Lys-Asp-Ser-Cys-Glu-Gly-Gly-Asp-Ser.

Hot tnh enzyme v c cht tc dng:

Papain thy phn peotein thnh cc polypeptide v cc polypeptide v
cc amino acid, ng vai tr va nh endopeptidase va nh
exopeptidase.
Cc endopeptidase thy phn protein ch yu thnh cc peptid:

Nhm 4

Trang 9

(-NH-CH(R)-CO-NH-CH(R)-CO-)n + HOH (-NH-CH(R)-COOH)I +

(H2N- CH(R)-CO-)k
i+k=n
Cc exopeptidase thy phn cc peptide thnh cc amino acid
(H2N-CH(R )-CO-NH-CH(R )-CO-)n + HOH (H2N-CH(R)COOH)n` + (H2N-CH(R)-CO-)k
n`+k = n
So vi cc protease c ngun gc ng vt v vi sinh vt khc th papain
c kh nng thy phn su hn. Tnh c hiu c cht ca papain rt
rng v n c kh nng phn hy hu ht cc lin kt peptide tr cc lin
kt vi proline v cc glutamic acid c nhm carboxy t do. Papain c
th nhn bit 1 chui gm 7 amino aicd trn c cht peptide ca mnh v
s u tin ct lin kt peptide trn 1 chui c phenylamine nh sau: nu
peptide c dng X-Phe-Y-Z (X, Y, Z l cc gc amino acid) th
papainsex ct ti v tr gia Y v Z, nhng nu peptide c dng X-Phe-Y
( c Phe nm v tr th 2 trc u tn cng) th khng c thy phn
bi papain.
Ngoi ra papain cn c hot tnh esterase, thiolesterase v trafnerase.
Hot ha papain:
Papain ch th hin hot tnh xc tc ca mnh khi nhm SH dng t
do. V vy ta s dng cht hot ha a papain t trng thi khng hot ng
sang trng thi hot ng. do trung tm hot ng ca papain c tnh kh nn
cc cht hot ha l cc cht c tnh kh nh cysteine, glytation aicd,
hdrocyanic trong cysteine l cht hay dng nht. hki c mt cc cht ny
th nhm SH ca papain c phc hi v lm tng hot tnh papain. thu
c hot tnh cao nht th thch hp l dng hn hp cysteine v EDTA, trong
cysteine ng vai tr l cht hot ha papain, cn EDTA ng vai tr cht
lin kt to phc vi ion kim loi nng c trong nha u .
Bt hot:
Nhm 4

Trang 10

Papain b km hm ( c ch bt thun nghch) bi cc cht oxy ha nh:

O2, O3, H2O2, iodur acetate, cysteine v cc hp cht disufur khc. Cc
cht ny phn ng vi nhm SH tring tm hot ng ca papain
lm ph v cu trc tm hot ng ca n.
Papain b bt hot thun nghch bi khng kh, cysteine nng thp.
papain tc dng vi chloromethul cetone ca Phe v Lys th mt hon
ton hot tnh. Tuy nhin papain li rt bn vi cc tc nhn bin tnh
l dung mi hu c ( quay cc ca papain hu nh khng bin i
trong ethanol 70% hay urea 6-8m).
d) Cc yu t nh hng n hot tnh xc tc ca papain
Nhit : Papain l enzyme chu c nhit tng i cao. dng
nha kh papain khng b bin tnh trong 3 gi 1000C. Cn dng dung dch
papain b mt hot tnh sau 30 pht 82.50C v nu nhit tng cao hn
(>1000C) th n s b mt hon ton hot tnh k c khi thm lng ln cht hot
ha vo dung dch.
iu ny l do dng dung dch khi tng ln n nhit ln hn 1000C
th cu trc tm hot ng ca papain b ph hy hon ton. iu ng lu l
sau khi c tinh sch v trng thi tinh th th papain c bn nhit thp
hn papain trong nha, do trong nha cn cha cc protein khc c tc dng
bo v papain.
Papain trong dung dch NaCl gi 40C bn trong nhiu thng. Trong
dung dch dn xut thy ngn, papain cng khng mt hot tnh trong nhiu
thng. Trong khi hu ht cc enzyme mt hot tnh mi ngy 1-2% do s
phn hy hoc oxy Khi thy phn cc protein khc nhau, th ty thuc vo c
cht m nhit thch hp cho papain cng khc nhau chng hn i vi c
cht l casein th nhit ti u cho phn ng l 370C. Papain dng n nh
trng thi kh c th chu nhit sy 1150C trong thi gian 2 gi m hot
tnh vn duy tr c 90%.

Nhm 4

Trang 11

pH: Papain hot ng trong khong pH tng i rng t 4.5-8.5 nhng

li d bin tnh trong mi trng acid c pH < 4.5 hoc trong mi trng kim
mnh c pH > 12. Khi phn ng vi c cht th ty thuc vo bn cht ca c
cht m pH ti u s khc nhau. Chng hn, papain phn ng vi casein pH
ti u l 7-7.5. Papain dng n nh tc l dng m cu trc khng gian ca
enzyme c n nh, c th chu c cc pH = 1.5 v pH = 8.5 trong 90 pht.
Dung mi: Papain khng thay i quay quang hc trong dung mi l
methanol 70% v khng thay i nht trong dung mi methanol 50%. Trong
dung dch dimethylsulfoxide cha 20% dung mi hu c v urea 8M khng lm
gim hot tnh cng nh thay i cu hnh ca papain. Cc cht gy bin tnh
mnh nh TCA 10%, guanidine hydrochloride 6M lm bin i bt thun
nghch v quay quang hc v hot tnh ca papain.

Nhm 4

Trang 12

CHNG III:
QUY TRNH CNG NGH SN XUT ENZYME
PAPAIN
3.1 nguyn liu
- Nguyn liu chnh thc v
cn hm lng ln nht l nha tri
u thu nhn t cc vn u .
- Celite.
- NaOH
- Ct sch
- (NH4)2SO4
- Mui NaCl (rn v lng)
vn u trng ly nha

3.2 thit b
- Ci
- Thit b lc
- Thit b lng
- Thit b sy

3.3 Quy trnh cng ngh

Nha cy u nm trong cc ng dn nha tri rng khp ton b cy,
ngoi tr r. nha l hn hp enzyme cha cc protease sau: papain,
chymopapain A( gc amino acid cui l glutamic acid), chymopapain B(gc
amino aicd l tyrosine), proteinase III, proteinase IV, thilprotesase, trong
papain chim ti 95%.

Nhm 4

Trang 13

iu kin ly nha :
- Cy ang sinh trng tt,
khng b su bnh,thn cy
mp mp cho nha nhiu.
- Qu : ly nhng qu cn
xanh,v mn,t 0.3 1kg,
10 tun tui l tt nht.
- Thi gian ly nha : sng
sm
Cch tin hnh :
- Ly nha qu xanh cn trn cy,dng dao inox u nhn rch
vi ng dc theo qu ch ng knh cao nht,cc lc kha
cch nhau 3 -5cm(khng rch su qu 2cm dch nc v tinh bt
t qu khng b trn ln vo nha lm gim cht lng nha).
- Hng ly nha chy ra bng l thy tinh mu ming rng 4-6 pht,
ly nha xong phi y np kn, gi trong ti v bo qun lnh
trong thi gian ch cc cng on x l tip theo. Mc ch ca
giai on ny l trnh khng cho nha tip xc lu vi khng kh,
m bo hot tnh papain c trong nha. Khi ly nha cn trnh
khng cho cc cht bn hoc cn trng ln vo.
Lu :
- Khng nn trn nha kh v nha ti v s lm gim cht lng
nha
- Qu c th c p nha trong sut 4 7 ngy, ln u c th ch
cn mt rch l , nhng ln thu nha sau rch 2 3 ng gia
nhng ng rch trc .
- Cn trnh khng cho nha ti tip xc vo da v n s gy
bng.khng nn nha tip xc vi cc dng c lm t kim loi
nng nh st, ng,v s lm bin mu v gim hot tnh
Nhm 4

Trang 14

enzyme. L, dao, mung,phi c lm bng nha hoc thp

khng r.
- Nha ti khng bn v th nn sy kh 5 % m.
3.3.1 Thu nhn papain
Chun b:
Do trong nha u ngoi papain, chymopapain cn c 1 s enzyme,
protein, cht nha, cht cao su, cht bo v cc tp cht khc c c quy
trnh tch v lm sch papain t nha u c hiu qu th c quy trnh ha tan
nha u nhm loi b tp cht. cch lm nh sau:
Trn 180g nha u vi 100g cetile v 150g ct sch, nghin k trong ci
nhit phng vi 200-300mL dung dch cysteine 0.04M (ha tan 6.3g cysteine
hydrochloride trong 1000mL dung dch NaOH 0.054M, kim c s dng
pH dch chit ti 5.7)
Khi nha to thnh th huyn ph th gn b lp ni trn. Qu trnh nghin
v trch c lp li vi 300mL dung dch cysteine. Ra ci vi dung dch
cysteine iu chnh th tch chit ln 11.
Dch huyn ph sau cng c lc lnh (c th ht nh) trn giy lc
Whatman s 1, thi gian lc c th rt di. Nc lc c mu trng sa hoc
vng xanh, pH gn 5.7.
Cc bc cn li ca qu trnh tinh sch enzyme c tin hnh trong iu
kin lnh.
Loi b cc cht khng tan pH9:
Thm t t v khuy u 1 lng khong 110ml dung dch NaOH 1M
iu chnh pH ca dch chit thu c trn ln pH 9. Lc hoc ly tm 2600
vng/pht trong 1 gi loi b ta xm to thnh (ta gy bin tnh protein),
thu dch trch trong.
Qu trnh phn on bng (NH4)2SO4:
Cho (NH4)2SO4 vo dch enzyme n 40% bo ha, sau 1-2 gi, ly tm
2500 vng/ pht. Thu nhn ta, b dch lng hoc c th gi li ly
Nhm 4

Trang 15

chymopapain. Ta c ra li 1 ln na vi 400-500ml dung dch (NH4)2SO4

40% bo ha.
Qu trnh phn on bng NaCl:
Ha tan trong 600mL dung dch cysteine 0.02M, dung dch thu c c pH
7- 7.5 sau thm t t 60g NaCl rn. Sau 1 gi, ly tm 2500 vng/pht trong
1 gi thu ta, b dich lng.
Kt tinh
Ha tan ta vi 400mL dung dch cysteine 0.002M nhit phng c 1
dung dch huyn ph c pH = 6.5. Gi nhit phng trong 30 pht tinh
th to thnh, duy tr 40C qua m, sau ly tm lnh 2500 vng/ pht
trong 4-5 gi thu nhn tinh th.
Ti kt tinh:
Ha tan tinh th thu c bc trn trong nc ct nhit phng to
dung dch c nng protein khong 1%. Sau cho vo t t ( ng thi
khuy u) dung dich NaCl bo ho ( 10mL/ 300mL dung dch protein). Khi
75% th tch dung dch NaCl cho vo, papain bt u kt tinh nhit
phng. Dch huyn ph c gi 40C qua m, sau ly tm lnh 2500 vng/
pht trong 4-5 gi thu nhn tinh th. Hot ng ring ca enzyme c th tng
nh nu tin hnh ti kt tinh thm 1 ln na nh trn.
tng hot tnh enzyme, trc khi ti kt tinh c th ha tan tinh th
papain trong dung dch m phosphate cha cysteine, sau tin hnh siu lc
vi mng lc 4000A0 v em kt tinh dch lng thu c sau siu lc.
Sy chn khng
Sy chn khng nhit 400C thu nhn papain tinh ch.
3.3.2 S quy trnh cng ngh

Nhm 4

Trang 16

Nguyn liu
180g nha u

Celite(100g)

200-300m dd cysteine
Nghin

0.04M

pH: 5.7
Ct sch(150g )

110mlNaOH 1M

Trch ly

Lc

Chnh pH

Lc
(NH4 )2 SO4

Kt ta phn on 1
Ly tm 1

60g NaCl rn

Kt ta phn on 2

Ta xm
Thi gian: 1-2 gi
400-500ml (NH4)2SO4
Dch lng

pH : 7-7.5
thi gian: 1 gi

Ly tm 2

Kt tinh

Dch lng
pH: 6.5
Thi gian: 30pht

dd NaCl bo
ha

Ti kt tinh

Sy chn khng

t: 37 0C
t: 40 0 C

Papain.

s quy trnh cng ngh

Nhm 4

Trang 17

3.4 ng dng ca enzyme papain.

3.4.1 Trong y hc:
Cha bnh giun kim, gium a.
Dng tiu dit cc d vt tha, cc protein cht trong c th. Hn hp
papain-bromelin-celluloza c s dng hiu qu ph si thn.
Lm thnh phn chnh ca cc loi thuc tr bnh bing n, n khng tiu.
Lm 1 s vi trng gram (+) v gram (-) ngng pht trin, vi trng thng
hn, Staphylococus rt nhy cm vi papain.
Papain c tc dng gim c t vi toxin v toxabumin:
Dung dch papain cysteine salicilate c cng dng cha bng.
Papain c tc dng ln h mch dng tr bnh bch cu, vim hng
3.4.2 Trong cng nghip thc phm:
- Protease, trong c papain c s dng rt rng ri trong ch bin tht,
c v mang li hiu qu kinh t cao. Vi phng php ny, ngi ta c th
bin i nhng loi tht c r tin, kh cng, nhng phn khng ngon thnh cc
ch phm c tnh cht tt hn.
- Papain cn c dng thy phn protein v ngn nga vi khun gy
thi, do nhit ti u ca n l 700C.
- Papain c dng lm mm tht, dng thy phn gan c ng lm
thuc b. Papain cn c dng trong sn xut bia v n gip tiu ha cc
protein cn ha tan trong bia.
3.4.3 Trong cc ngnh cng nghip khc:
- Papain c dng lm mm da trong ngnh cng nghip thuc da, dng ty
cc vt mu trn qun o.
- Trong cng nghip m phm, papain c dng ty cc vt nm, tn
nhang trn da lm cho da mm mi hn.

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Loi mt n dng da

ch phm enzyme

c cha enzyme papain

v nhng dng cht
ca u

CHNG IV:
KT LUN
Vic sn xut ch phm enzyme cc loi v ang pht trin mnh m trn
qui m cng nghip. Hin ti c hng nghn ch phm enzyme bn trn th
trng th gii,

Cc ch phm enzyme ph bin nh amylase, protease,

catalase,cellulase,lipase,glucoseoxydase
Ch phm enzyme khng ch c ng dng trong y hc m cn c ng
dng trong nhiu lnh vc cng nghip khc nhau, trong nng nghip, trong ha
hc ngha ca vic s dng enzyme trong cc lnh vc thc t khng km
so vi ngha ca vic s dng nng lng nguyn t. c nhng nghin
cu ng dng ca enzyme papain trong thy phn u nnh, trong ch bin tht,
trong sn xut m phm

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CHNG V:
TI LIU THAM KHO
1. http://www.docsachonline.vn/thu-nhan-enzyme-papain-tu-du-du
2. http://tailieu.vn/xem-tai-lieu/bao-cao-ung-dung-enzyme-papain-trongthuy-phan-banh-dau-dau-phong-nham-tao-san-pham-co-gia-tridin.659842.html
3. http://tailieu.vn/tinh-chat-hoa-hoc-cua-enzyme-papain
4. http://vho.vn/cac-duoc-pham-co-papain
5. Bi ging K thut thc phm III-trng i Hc Nng Lm tp.HCMTS.Bi Anh Vit.

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