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  • Tugas Mikrobiologi: Grafik Curea/-R vs. Curea

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    Tirza Kasamira Frederica
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    This document describes an experiment to determine the Michaelis-Menten constants Km and Vmax for urea using Hanes plot analysis. Various urea concentrations were tested to determine reaction rates. A graph of urea divided by reaction rate versus urea concentration was plotted, yielding a linear relationship. The slope and intercept of the line were used to calculate Km and Vmax, found to be 0.023959342 kmol/m3 and 1.210067764 kmol/m3.sec respectively.

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    growth rate bacteria

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    Tugas Mikrobiologi

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    This document describes an experiment to determine the Michaelis-Menten constants Km and Vmax for urea using Hanes plot analysis. Various urea concentrations were tested to determine reaction rates. A graph of urea divided by reaction rate versus urea concentration was plotted, yielding a linear relationship. The slope and intercept of the line were used to calculate Km and Vmax, found to be 0.023959342 kmol/m3 and 1.210067764 kmol/m3.sec respectively.

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    11 views3 pages

    Tugas Mikrobiologi: Grafik Curea/-R vs. Curea

    Uploaded by

    Tirza Kasamira Frederica
    This document describes an experiment to determine the Michaelis-Menten constants Km and Vmax for urea using Hanes plot analysis. Various urea concentrations were tested to determine reaction rates. A graph of urea divided by reaction rate versus urea concentration was plotted, yielding a linear relationship. The slope and intercept of the line were used to calculate Km and Vmax, found to be 0.023959342 kmol/m3 and 1.210067764 kmol/m3.sec respectively.

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    © All Rights Reserved
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    TUGAS MIKROBIOLOGI

    Tirza Kasamira Frederica


    Teknik Kimia/114.12.0021

    Menentukan Km dan Vmax dengan menggunakan Hanes Plot


    Curea
    (kmol/m3)
    -r
    (kmol/m3.j)
    Curea/-r

    0.2

    0.02

    0.01

    1.08
    0.185
    185

    0.55
    0.036
    364

    0.38
    0.026
    316

    0.00
    5

    0.002

    0.2
    0.09
    0.02 0.0222
    5
    22

    Grafik Curea/-r vs. Curea


    0.2
    f(x) = 0.83x + 0.02
    R = 1

    0.15

    Curea/ -r

    0.1
    0.05
    0
    0

    0.05

    0.1

    Curea

    Menghitung slope secara manual:


    Slope

    y5 y1
    x5 x1

    0.0222220.185185
    0.0020.2

    0.15

    0.2

    0.25

    Slope

    = 0.823045455

    Menghitung Km dan Vmax berdasarkan Hanes Plot (Grafik Curea/-r vs.


    Curea)
    y=ax+b

    C urea
    K
    1
    =
    Curea + m
    r V max
    V max

    Dari grafik diketahui:


    Slope

    = 0.8264

    Intercept

    = 0.0198

    Slope

    1
    V max

    Vmax

    1
    slope

    1
    0.8264

    Vmax=1.210067764

    Intercept

    Km

    Km
    V max
    =

    Vmaxintercept

    Km

    1.2100677640.0198

    0.023959342

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