Phng phap chun complexon

Muc ich: trang bi cho sinh vin kin thc cung nh ki nng thc nghi m phng phap chun
complexon ( chun tao phc ).
Nguyn tc: Phng phap chun complexon (C) la phng phap chun tap phc s dung thuc th
complexon chun cac ion kim loai ( M ), theo cn bng tao phc: M + C c MC
Complexon la cac axit aminopolycacboxylic, trong o c ng dung r ng rai nht la EDTA
EDTA dang axit it tan trong nc, vi v y ngi ta thng dung di dang mui dinatri complexon III, ki
hi u Na2h2Y
Complexon III hay

Trilon

B (Na2C10H14N2O8) la mui dinatri ca axit

etylendiamintetraaxetic (EDTA) co cng thc cu tao:

Ki hi u la Na2h2Y.
Trong phan ng Mn+ H2Y2- MY(4-n) + 2H+
Tao ra H+ nn pH giam trong qua trinh phan ng, n inh ngoi ta dung dung dich m ( trong bai
chon dung dich m la NH3 c va NH4Cl ).
M t s cht chi thi thng dung Ericrom T en ( ETOO), murexit
Thi nghi m 1: Pha dung dich chun EDTA ( 1 ban ai di n lam cho ca lp )
Thi nghi m 2: Xac inh nng Mg2+ bng dung dich EDTA
Dung cu va hoa cht s dung:
Dung cu

Hoa cht

Chi thi

Pipet bu 10ml

Dung dich Mg2+

ETOO

Binh non 250ml

Dung dich m
amoniac

Buret chun t

Dung dich EDTA

ng 50ml

Cac bc tin hanh:

Bc 1: ra cac dung cu thi nghi m bng nc sau o trang lai bng nc ct, sau o dung dung dich
EDTA trang lai buret chun t ng loai bo nc ri thm EDTA n vach 0 ca buret bng qua
bop.

Bc 2: Dung pipet bu ly 10,00ml dung dich Mg2+ cn xac inh vao binh non 250ml, sau o thm 5ml
dung dich m amoniac.
Bc 3: Cho m t it ETOO vao binh non 250ml trn ( khoang 40% thia ) va lc u, dung dich Mg2+
chuyn sang mau o nho.
Bc 4: Chun bng EDTA t buret n khi dung dich chuyn sang mau xanh.
L p lai thi nghi m 3 ln, ghi lai th tich ca dung dich EDTA va x ly s li u thu c.
Bao cao kt qua
Th tich ca dung dich EDTA:
Ln 1: V1= 22,00ml
Ln 2: V2= 22,10ml
Ln 3: V3= 22,10ml

v TB =
=>

V1 + V2 + V3 22, 00 + 22,10 + 22,10

=
= 22, 07 (ml).
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Thi nghi m 3: inh lng Mg2+ trong vin nen Mgnesi-B6

Dung cu va hoa cht s dung
Dung cu

Hoa cht

Chi thi

Cn phn tich

1 vin nen Mgnesi-B6

ETOO

Cc thy tinh
Pipet bu 10ml

Binh inh mc 50ml

Binh non 250ml

Buret chun t

Dung dich EDTA

ng 50ml
Cac bc tin hanh:
Bc 1: Ra sach cac dung cu thi nghi m ( tr cc cn), ly 1 vin nen Mgnesi-B6 ( a nghin nho )
vao cc thy tinh ri em i cn trn cn phn tich.
Bc 2: Thm 20ml nc ct vao cc thy tinh, khuy 2-3 phut cho vin nen tan dn ri vao binh
inh mc 50ml.
Bc 3: Dung pipet bu ly 10,00ml dung dich thu c cho vao binh non 250ml, thm khoang 40%
thia chi thi ETOO, lc u ri chun bng dung dich EDTA t buret.
L p lai thi nghi m 3 ln, ghi lai th tich ca dung dich EDTA va x ly s li u thu c.
Bao cao kt qua:
mvin nen = 0,5297g
Th tich ca dung dich EDTA a chun :

Ln 1: V1= 2,80ml
Ln 2: V2= 2,60ml
Ln 3: V3= 2,70ml

VTB
=>

V1 V2 V3 2,80 2, 60 2, 70

2, 70 (ml).
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Thi nghi m 4: Xac inh nng Ca2+ va Mg2+ trong m t hn hp bng dung dich EDTA.
Dung cu va hoa cht s dung:
Dung cu

Hoa cht

Chi thi

Pipet bu 10ml

Dung dich Mg2+ va

ETOO ( thi nghi m

Ca2+

4a )

Dung dich m

Murexit ( thi nghi m

amoniac

4b )

Binh non 250ml

Buret chun t

Dung dich EDTA

ng 50ml
Dung dich NaOH 1M
Thi nghi m 4a: Dung chi thi ETOO
Cac bc tin hanh:
Bc 1: Ra cac dung cu thi nghi m, dung pipet bu ly 10,00ml dung dich Mg2+ va Ca2+ cho vao binh
non 250ml, sau o thm 5ml dung dich m amoniac.
Bc 2: Cho m t it ETOO vao binh non 250ml trn ( khoang 40% thia ) va lc u, dung dich Mg2+ va
Ca2+ chuyn sang mau o nho.
Bc 3: Chun bng dung dich EDTA t buret ti khi dung dich chuyn t mau o nho sang mau
xanh.
L p lai thi nghi m 3 ln, ghi lai th tich ca dung dich EDTA va x ly s li u thu c.
Th tich dung dich EDTA a chun :
Ln 1: V1= 19,80ml
Ln 2: V2= 19,70ml
Ln 3: V3= 19,80ml
VTB

V1 V2 V3 19,80 19, 70 19,80

19, 77 (ml).
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Thi nghi m 4b: Dung chi thi Murexit

Cac bc tin hanh:
Bc 1: Ra cac dung cu thi nghi m, dung pipet bu ly 10,00ml dung dich Mg2+ va Ca2+ cho vao binh
non 250ml..

Bc 2: Thm 1ml dung dich NaOH 1M va m t lng chi thi Murexit vao binh non 250ml trn ( khoang
40% thia ) va lc u, thy xut hi n kt ta Mg(OH)2 mau trng.
Bc 3: Chun bng dung dich EDTA t buret ti khi dung dich chuyn t mau o nho sang mau tim.
L p lai thi nghi m 3 ln, ghi lai th tich ca dung dich EDTA va x ly s li u thu c.
Ln 1: V1= 8,90ml
Ln 2: V2= 8,80ml
Ln 3: V3= 9,00ml
VTB

V1 V2 V3 8,90 8,80 9, 00

8,90 (ml).
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