EQUIPMENT NAME: FIXED BED REACTOR

EQUIPMENT CODE:

Activated Carbon
Adsorption J-6
Capacity: 0.7

MSG Solution Clear MSG Solution to

from J-5 T-1

MSG = 2,612.24 kg
Maltose = 136.24 kg
Oligosaccharides = 66.55 kg
Water = 66,334.62 kg
C. Glutamicum = 77.58 kg E-20
MSG = 2,545.13 kg
Amylase = 0.09 kg 1N HCl = 0.16 kg
Glucoamylase = 0.05 kg Water = 64,630.49 kg
Pullulanase = 0.04 kg Lysine = 782.66 kg
CaCl2 = 0.001 kg
TOTAL 2,112.32kg
NaOH = 29.89 kg
Lysine = 803.29 kg
Protein = 6.59 kg
Fats = 2.40 kg
Fibre = 1.01 kg
1N HCl = 0.16 kg
Total = 70,070.75 kg
Waste Material

MSG = 67.11 kg
Maltose = 136.24 kg
Oligosaccharides = 66.55 kg
Water = 1,704.14 kg
Amylase = 0.09 kg
Glucoamylase = 0.05 kg
Pullulanase = 0.04 kg
CaCl2 = 0.001kg
NaOH = 29.89 kg
Lysine = 20.64 kg
Protein = 6.59 kg
Total 67,958.43 kg
Mass In: Mass Out:

MSG Solution 70,214.79 kg Clear MSG Solution 68,098.13 kg

Waste Material 2,116.66 kg

Total Mass In = 70,214.79 kg Total Mass Out = 70,214.79 kg

Detailed Computation:

Inlet Stream

MSG Solution

Mass of MSG = 3.73% (70,214.79 kg) = 2,612.61 kg

Mass of Maltose = 0.19% (70,214.79 kg) = 136.52 kg

Mass of Oligosaccharide = 0.09% (70,214.79 kg = 66.69 kg

Mass of Water = 94.67% (70,214.79 kg) = 66,470.98 kg

Mass of Corynebacterium = 0.11% (70,214.79 kg) = 77.74 kg

Mass of Amylase = 0.000125% (70,214.79 kg) = 0.09 kg

Mass of Glucoamylase = 0.000073% (70,214.79 kg) = 0.05 kg

Mass of Pullulanase = 0.000062% (70,214.79 kg) = 0.04 kg

Mass of CaCl2 = 0.000001% (70,214.79 kg) = 0.001 kg

Mass of NaOH = 0.04% (70,214.79 kg) = 29.94 kg

Mass of Lysine = 1.15% (70,214.79 kg) = 804.95 kg

Mass of Protein = 0.01% (70,214.79 kg) = 6.60 kg

Mass of Fats = 0.0034% (70,214.79 kg) = 2.40 kg

Mass of Fibre = 0.0015% (70,214.79 kg) = 1.02 kg

Mass of 1N HCl = 0.0002% (70,214.79 kg) = 0.16 kg

70,214.79 kg
Outlet Stream

Clear MSG Solution

Mass of MSG = 3.75% (68,098.13 kg) = 2,550.36 kg

Mass of 1N HCl = 0.00023% (68,098.13 kg) = 0.16 kg

Mass of Water = 95.10% (68,098.13 kg) = 64,763.34 kg

Mass of Lysine = 1.15% (68,098.13 kg) = 784.27 kg

68,098.13 kg

Waste Material

Mass of MSG = 3.18% (2,116.66 kg) = 67.24 kg

Mass of Maltose = 6.45% (2,116.66 kg) = 136.52 kg

Mass of Oligosaccharide = 3.15% (2,116.66 kg) = 66.69 kg

Mass of Water = 80.68% (2,116.66 kg) = 1,707.64 kg

Mass of Amylase = 0.00414% (2,116.66 kg) = 0.09 kg

Mass of Glucoamylase = 0.00242% (2,116.66 kg) = 0.05 kg

Mass of Pullulanase = 0.00207% (2,116.66 kg) = 0.04 kg

Mass of CaCl2 = 0.00004% (2,116.66 kg) = 0.001 kg

Mass of NaOH = 1.41% (2,116.66 kg) = 29.95 kg

Mass of Lysine = 0.98% (2,116.66 kg) = 20.68 kg

Mass of Protein = 0.31% (2,116.66 kg) = 6.60 kg

Mass of Fats = 0.11% (2,116.66 kg) = 2.40 kg

Mass of Fibre = 0.05% (2,116.66 kg) = 1.02 kg

Mass of 1N HCl = 0.0002% (2,116.66 kg) = 0.004 kg

Mass of Corynebacterium = 3.67% (2,116.66 kg) = 77.73 kg

2,116.66 kg
Overall Mass Balance

MSG Solution = Clear MSG Solution + Waste Material

70,214.79 kg = 68,098.13 kg + 2,116.66 kg

70,214.79 kg = 70,214.79 kg
EQUIPMENT NAME: CRYSTALLIZER

EQUIPMENT CODE: T-1

Clear MSG Solution from MSG Magma to

J-6 K-5
T-1
MSG = 2,550.36 kg
1N HCl = 0.16 kg MSG Crystals = 21.065 %
Water = 64,763.34 kg MSG H2O = 2.341 %
Lysine = 784.27 kg 1N HC = 0.16 kg
TOTAL = 68,098.13 kg Water = 64,763.34 kg
Lysine = 784.27 kg
TOTAL = 68,098.13 kg

Mass In: Mass Out:

Clear MSG Solution 68,098.13 kg MSG magma 66,145.94 kg

Water 1,952.19 kg

Total Mass In = 68,098.13 kg Total Mass Out = 68,098.13 kg

Detailed Computation:

Inlet Stream

Clear MSG Solution

Mass of MSG = 3.75% (68,098.13 kg) = 2,550.36 kg

Mass of 1N HCl = 0.00023% (68,098.13 kg) = 0.16 kg

Mass of Water = 95.10% (68,098.13 kg) = 64,763.34 kg

Mass of Lysine = 1.15% (68,098.13 kg) = 784.27 kg

68,098.13 kg

Outlet Stream

Water

Mass of Water = 7% (64,763.34 kg - 2,581.24 kg)

= 1,952.19 kg

MSG magma

Mass of MSG Crystals = 7.76% (66,145.94 kg) = 5,131.61 kg

Mass of MSG H2O = 3.82% (66,145.94 kg) = 2,581.24 kg

Mass of 1N HCl = 0.00024% (66,145.94 kg) = 0.16 kg

Mass of Water = 87.15% (66,145.94 kg) = 57,648.66 kg

Mass of Lysine = 1.19% (66,145.94 kg) = 784.27 kg

66,145.94 kg

Overall Mass Balance

MSG Solution = MSG magma + Water

68,098.13 kg = 66,145.94 kg + 1,952.19 kg

68,098.13 kg = 68,098.13 kg
EQUIPMENT NAME: CENTRIFUGE

EQUIPMENT CODE: K-5

MSG Magma from Wet MSG Crystals

T-1 to E-4
K-5

MSG Crystals = 5,131.61 kg

MSG Crystals = 5,131.61 kg
MSG H2O = 2,581.24 kg
MSG H2O = 2,581.24 kg
1N HCl = 0.16 kg
1N HCl = 0.16 kg
Water = 57,648.66 kg
Water = 57,648.66 kg
Lysine = 784.27 kg
Lysine = 784.27 kg
TOTAL = 66,145.94 kg
TOTAL = 66,145.94 kg

Mother Liquor
MSG 0.277 %
Water 89.919 %
Soluble Impurities
Glucose 5.947 %
Maltose 0.272 %
Oligosaccharides 0.197 %
Lysine 3.387 %
Total kg

Mass In: Mass Out:

MSG magma 66,145.94 kg Wet MSG Crystals 12,784.88 kg

Mother Liquor 53,361.06 kg

Total Mass In = 66,145.94 kg Total Mass Out = 66,145.94 kg

Detailed Computation:

Inlet Stream

MSG magma

Mass of MSG Crystals = 7.76% (66,145.94 kg) = 5,131.61 kg

Mass of MSG H2O = 3.82% (66,145.94 kg) = 2,581.24 kg

Mass of 1N HCl = 0.00024% (66,145.94 kg) = 0.16 kg

Mass of Water = 87.15% (66,145.94 kg) = 57,648.66 kg

Mass of Lysine = 1.19% (66,145.94 kg) = 784.27 kg

66,145.94 kg

Outlet Stream

Mother Liquor

Mass of MSG Crystals = 0.96% (53,361.06 kg) = 513.16 kg

Mass of MSG H2O = 0.48% (53,361.06 kg) = 258.12 kg

Mass of 1N HCl = 0.00027% (53,361.06 kg) = 0.14 kg

Mass of Water = 97.23% (53,361.06 kg) = 51,883.80 kg

Mass of Lysine = 1.32% (53,361.06 kg) = 705.84 kg

53,361.06 kg

Wet MSG Crystals

Mass of MSG Crystals = 0.96% (12,784.88 kg) = 6,941.56 kg

Mass of 1N HCl = 0.00027% (12,784.88 kg) = 0.02 kg

Mass of Water = 97.23% (12,784.88 kg) = 5,764.87 kg

Mass of Lysine = 1.32% (12,784.88 kg) = 78.43 kg

12,784.88 kg
Overall Mass Balance

MSG magma = Wet MSG crystals + Mother liquor

66,145.94 kg = 12,784.88 kg + 53,361.06 kg

66,145.94 kg = 66,145.94 kg
EQUIPMENT NAME: TRAY DRIER

EQUIPMENT CODE: E-4

Hot Dry Air from

E-3

Wet MSG Crystals

from K-5 MSG to K-6
E-4
MSG 87.035 % MSG 96.773 %
MSG H2O 9.768 % Water 2.910 %
Water 2.882 % Impurities
Soluble Impurities Glucose 0.192 %
Glucose 0.191 % Maltose 0.009 %
Maltose 0.009 % Oligosaccharides 0.006 %
Oligosaccharides 0.006 % Lysine 0.110 %
Lysine 0.109 % Total kg
Total kg

Humid Air

Mass In: Mass Out:

Wet MSG Crystals 12,784.88 kg MSG 7000 kg

Evaporated 5,784.88 kg

Total Mass In = 12,784.88 kg Total Mass Out = 12,784.88 kg

Detailed Computation:

Inlet Stream

Wet MSG Crystals

Mass of MSG Crystals = 0.96% (12,784.88 kg) = 6,941.56 kg

Mass of 1N HCl = 0.00027% (12,784.88 kg) = 0.02 kg

Mass of Water = 97.23% (12,784.88 kg) = 5,764.87 kg

Mass of Lysine = 1.32% (12,784.88 kg) = 78.43 kg

12,784.88 kg

Outlet Stream

MSG

Mass of MSG Crystals = 99.17% (7000 kg) = 6,941.57 kg

Mass of Water = 0.824% (7000 kg) = 57.65 kg

Mass of Lysine = 0.011% (7000 kg) = 0.78 kg

7000 kg

Evaporated

Mass of Water = 98.66% (5,784.88 kg) = 5,707.22 kg

Mass of 1N HCl = 0.00027% (5,784.88 kg) = 0.02 kg

Mass of Lysine = 1.34% (5,784.88 kg) = 77.64 kg

5,784.88 kg

Overall Mass Balance

Wet MSG crystals = MSG + Evaporated

12,784.88 kg = 7000 kg + 5,784.88 kg

12,784.88 kg = 12,784.88 kg
EQUIPMENT NAME: GRINDER

EQUIPMENT CODE: K-6

 MSG 769.70 kg
Water 6.39 kg
MSG Crystals Lysine 0.09 kg____
from K-7 Total 776.18 kg

MSG from E-4 MSG Crystals to K-7

K-6

MSG 6,927.33 kg MSG 7,697.03 kg

Water 57.53 kg Water 63.92 kg
Lysine 0.78 kg____ Lysine 0.87 kg____
Total 6,985.64 kg Total 7,761.82 kg

Mass In: Mass Out:

MSG 6,985.64 kg MSG Crystals 7,761.82 kg

Screened MSG 776.18 kg

Total Mass In = 7,761.82 kg Total Mass Out = 7,761.82 kg

Assumptions:

No losses

MSG + MSGGRIND = MSGOVERSIZED

Inlet Stream

MSG
Mass:

Mass of MSG Crystals = 99.17% (7000 kg) = 6,941.57 kg

Mass of Water = 0.824% (7000 kg) = 57.65 kg

Mass of Lysine = 0.011% (7000 kg) = 0.78 kg

7,000 kg

MSGOVERSIZED

Mass:

MSG = 694.16 kg

Water = 5.77 kg

Lysine = 0.07 kg_

700 kg

Outlet Stream

MSG = 7635.73 kg

Water = 63.42 kg

Lysine = 0.85 kg_

7,700 kg

MSG + MSGGRIND = MSGOVERSIZED

7,000 kg + 700 kg = 7,700 kg

7,700 kg = 7,700 kg
EQUIPMENT NAME: SCREENER

EQUIPMENT CODE: K-7

MSG Crystals MSG = 694.16 kg

To K-6 Water = 5.77 kg
Lysine = 0.07 kg____
Total 700 kg

MSG Crystals
MSG from K-6

MSG = 6,941.57 kg K-7 MSG = 6,247.39 kg

Water = 57.65 kg Water = 51.91 kg
Lysine = 0.78 kg____ Mesh 60 Lysine = 0.70 kg___
Total 7,000 kg Total 6,300 kg

Mass In: Mass Out:

Ground MSG Crystals 7,700 kg Screened MSG Crystals 7,000 kg

Oversized MSG Crystals 700 kg

Total Mass In = 7,700 kg Total Mass Out = 7,700 kg

Assumption:

10% of feed processed as ovesized

Detailed Computations:
Feed:

0.5 mm size MSG Crystals

MSG = 7635.73 kg

Water = 63.42 kg

Lysine = 0.85 kg_

7,700 kg

Overall Mass Balance:

= +

Initial Computation:

Oversized Crystals (>0.248 mm) = 10% of Ground Crystals

= (0.10) * (7,700 kg)

= 770 kg

Screened Crystals (0.248 mm) = 90% of Ground Crystals

= (0.90) * (7,700 kg)

= 6,930 kg

Overall Mass Balance:

=
 6,930kg = 7,700 kg 770 kg

Product:

Screened MSG Crystals Oversized MSG Crystals

MSG = 6,247.39 kg MSG = 694.16 kg

Water = 51.91 kg Water = 5.77 kg

Lysine = 0.70 kg Lysine = 0.07 kg

Total = 6,300 kg Total = 700 kg