1.

An experiment was carried out to study the effect of enzyme concentration on the rate of reaction
of amylase enzyme.
Diagram 1 shows the apparatus set-up to study the effect of enzyme concentration on the rate of
reaction of amylase enzyme.
Satu eksperimen telah dijalankan untuk mengkaji kesan kepekatan enzim ke atas kadar tindak
balas enzim amilase.
Rajah 1 menunjukkan susunan radas untuk mengkaji kesan kepekatan enzim ke atas kadar
tindak balas enzim amilase.

Water bath 37°C

0
Kukus air 37 C
Amylase + starch
Amilase + kanji

Diagram 1
Rajah 1
The following steps were carried out:
Langkah-langkah berikut telah dijalankan:

Step 1 : 2 ml of 0.2% amylase solution is filled in a test tube.

Langkah 1 : 2ml ampaian kanji 0.2% dimasukkan ke dalam tabung uji.

Step 2 : 2 drops of iodine solution is placed into each groove on a white tile.
Langkah 2 : 2 titis larutan iodin diletakkan ke dalam setiap lekuk pada suatu jubin putih.

Step 3 : 2 ml of 1% starch suspension is added into the test tube and the stopwatch is
started immediately.
Langkah 3 : 2 ml 1% ampaian kanji ditambah kedalam tabung uji itu dan jam randik
dimulakan serta merta.

Step 4 : Every 2 minutes, a drop of the mixture from the test tube is placed into a new
groove of iodine solution on the white tile by using a clean dropper.
Langkah 4 : Setiap 2 minit setitik campuran daripada tabung uji itu dititiskan ke atas lekuk
baharu yang mengandungi larutan iodin pada jubin putih itu dengan
menggunakan penitis yang bersih.

Step 5 : The time taken for the iodine solution to remain yellow is recorded.
Langkah 5 : Masa diambil untuk larutan iodin kekal kuning dicatatkan.

Step 6 : Steps 1-5 are repeated using 0.4%, 0.6% and 0.8% concentration of amylase
solution.
Langkah 6 : Langkah 1-5 diulang dengan menggunakan larutan amilase pada kepekatan
0.4%, 0.6% dan 0.8%.
1
Table 2 shows the results of this experiment
Jadual 2 menunjukkan keputusan eksperimen ini.
Percentage Observation Time taken for iodine
concentration Pemerhatian solution to remain
of amylase
yellow (min)
solution (%)
Masa diambil untuk
Peratus End of experiment
larutan iodin kekal kuning
kepekatan Akhir eksperimen
(min)
larutan
amilase (%)

………….. minutes
0.2
minit

12 minutes
0.4
12 minit

………….. minutes
0.6
minit

………….. minutes
0.8
minit

Table 1
Jadual 1

2
(a) Record the time taken for iodine solution to remain yellow in Table 1.
Rekodkan masa yang diambil untuk larutan iodin kekal kuning dalam Jadual 1.
[3 marks]
[3 markah]

(b) (i) Based on Table 1, state two different observations.

Berdasarkan Jadual 1, nyatakan dua pemerhatian yang berbeza.

Observation 1:
Pemerhatian 1:

…………………………………………………………………………………………………..

…………………………………………………………………………………………………..
Observation 2:
Pemerhatian 2:

…………………………………………………………………………………………………..

…………………………………………………………………………………………………..
[3 marks]
[3 markah]

(ii) State two inferences which correspond to the observation in 1(b)(i).

Nyatakan dua inferens yang sepadan dengan pemerhatian di 1(b)(i).

Inference from observation 1:

Inferens daripada pemerhatian 1:

…………………………………………………………………………………………………..

…………………………………………………………………………………………………..

Inference from observation 2:

Inferens daripada pemerhatian 2:

…………………………………………………………………………………………………..

…………………………………………………………………………………………………..
[3 marks]
[3 markah]

3
(c) Complete Table 2 based on this experiment.
Lengkapkan Jadual 2 berdasarkan eksperimen ini.

Variable Method to handle the variable

Pembolehubah Cara mengendalikan pembolehubah

Manipulated variable
Pembolehubah dimanipulasikan

.............................................................. ..............................................................

.............................................................. ..............................................................

.............................................................. ..............................................................
Responding variable
Pembolehubah bergerak balas

.............................................................. ..............................................................

.............................................................. ..............................................................

.............................................................. ..............................................................
Constant variable
Pembolehubah dimalarkan

.............................................................. ..............................................................

.............................................................. ..............................................................

.............................................................. ..............................................................
Table 2
Jadual 2
[3 marks]
[3 markah]

(d) State the hypothesis for the experiment.

Nyatakan hipotesis bagi eksperimen ini.

…………………………………………………………………………………………………………

…………………………………………………………………………………………………………
[3 marks]
[3 markah]

4
(e) (i) Construct a table and record all the data collected from the experiment.
Your table should have the following titles.

 Percentage concentration of amylase enzyme

 The time taken for iodine solution to remain yellow

 The rate of enzyme reaction

1
Rate of enzyme reaction = ______________________________________
Time taken for iodine solution to remain yellow(minutes)

Bina satu jadual dan rekodkan semua data yang dikumpul daripada eksperimen itu.
Jadual anda hendaklah mengandungi tajuk-tajuk berikut:

 Peratus kepekatan larutan enzim amilase

 Masa yang diambil untuk larutan iodin kekal kuning

 Kadar tindak balas enzim

1
Kadar tindak balas enzim = _________________________________________
Masa yang diambil untuk larutan iodin kekal kuning (minit)

[3 marks]
[3 markah]

(ii) Using the data in 1(f)(i), draw a graph of the rate of amylase reaction against the
concentration of amylase solution.
Menggunakan data di 1(f)(i), lukiskan graf bagi kadar tindak balas amilase melawan
kepekatan larutan amilase.
[3 marks]
[3/ markah]

5
(f) Based on the graph in 1(e)(ii),state the relationship between the rate of reaction of amylase
and concentration of amylase solution.
Explain your answer.
Berdasarkan graf di 1(e)(ii), nyatakan hubungan antara kadar tindak balas amilase dan
kepekatan larutan amilase.
Terangkan jawapan anda.

…………………………………………………………………………………………………………

…………………………………………………………………………………………………………

…………………………………………………………………………………………………………

…………………………………………………………………………………………………

…………………………………………………………………………………………………
[3 marks]
[3 markah]

(g) Based on the result of the experiment, state the operational definition for rate of reaction of
amylase enzyme.
Berdasarkan keputusan eksperimen itu, nyatakan definisi secara operasi bagi kadar tindak
balas enzim amilase.

…………………………………………………………………………………………………………

…………………………………………………………………………………………………………

…………………………………………………………………………………………………………
[3 marks]
[3 markah]

(h) The experiment is repeated with test tube containing 0.8% concentration of amylase enzyme
immersed in water bath with temperature of 50°C during the experiment.
Predict the time taken for starch to be hydrolysed completely in this test tube.
Explain your prediction.
Eksperimen diulang dengan merendam tabung uji dengan kepekatan enzim amilase 0.8%
dalam kukus air bersuhu 50°C semasa eksperimen.
Ramalkan masa yang diambil bagi kanji dihidrolisiskan dengan lengkap dalam tabung uji ini.
Terangkan ramalan anda.

…………………………………………………………………………………………………………

…………………………………………………………………………………………………………

…………………………………………………………………………………………………………
[3 marks]
[3 markah]

6
(i) The following list are the apparatus and materials used in eksperiment.
Senarai berikut ialah radas dan bahan yang digunakan dalam eksprimen.

Starch suspension Water bath

Ampaian kanji Kukus air

Syringes White tiles with grooves

Picagari Jubin putih berlekuk

Test tubes Iodine solution

Tabung uji Larutan iodin

Classify the apparatus and materials in Table 3.

Kelaskan radas dan bahan dalam Jadual 3.

Material Apparatus
Bahan Radas

Table 3
Jadual 3
[3 marks]
[3 markah]

7
2. Leptospirosis is a disease that is caused by infected rat urine. Ingesting food or water
contaminated with this rat urine can cause the disease.
Studies done have shown that many residents from Taman Megah, have been infected with
this disease.
Leptospirosis adalah sejenis penyakit yang disebabkan oleh kencing tikus. Pengambilan
makanan atau air yang dicemari dengan kencing tikus menyebabkan penyakit ini.
Kajian yang dijalankan telah menunjukkan ramai penghuni dari Taman Megah telah
dijangkiti dengan penyakit ini .

Diagram 2(a) shows the rat species (Rattus rattus) found in Taman Megah.
Diagram 2(b) shows the map of Taman Megah.
Rajah 2(a) menunjukkan spesies tikus (Rattus rattus) yang dijumpai di Taman Megah.
Rajah 2(b) menunjukkan peta Taman Megah.

Restaurant/Restoran School/ Sekolah

Restoran

Factory/Kilang
Diagram 2(a)/ Rajah 2(a) Diagram 2(b) / Rajah 2(b)

Based on the above information, plan an experiment to study the population size of the rat at
different locations in Taman Megah.
Berdasarkan maklumat di atas, rancang satu eksperimen untuk mengkaji saiz populasi tikus
di tempat yang berlainan di Taman Megah.

The planning of your experiment must include the following aspects

Perancangan eksperimen anda hendaklah mengandungi aspek-aspek berikut
 Problem statement
Pernyataan masalah
 Hypothesis
Hipotesis
 Variables
Pembolehubah
 List of apparatus and materials
Senarai radas dan bahan
 Experimental procedure
Prosedur eksperimen
 Presentation of data
Persembahan data [ 17 marks/17 markah]

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