BJM Carmel Academy

Senior Secondary School

(2020-2021)

A Project Report On
Investigatory project in Biology (044)
Effect of antibiotics on micro organisms

Submitted by:
Mas. Bhuvansh D. Gorghate
-
Guided by:
Mrs. Sunita Mehta

Roll No.5 Date:-

 BJM Carmel Academy
Senior Secondary School
(2020-2021)

Certificate
This is to certify that Mas. Bhuvansh D. Gorghate has
successfully completed project titled “EFFECT OF
ANTIBIOTICS ON MICRO ORGANISMS” In partial
fulfillment of requirement for Std. XII certificate of
Central Board of Secondary Education (CBSE), New
Delhi, in subject Biology(044),for the academic session
2020-2021.This project is a record of work carried out by
him under my guidance.
Subject Teacher stamp Principal

Mrs. Sunita Mehta

Date: Roll No.5

 BJM Carmel Academy
Senior Secondary School
(2020-2021)

Declaration
To,
The Principal
BJM Carmel Academy
Chandrapur.
Respected Fr. Principal,
I, Mas Bhuvansh D. Gorghate hereby inform that I am submitting the
project report entitled “EFFECT OF ANTIBIOTICS ON MICRO
ORGANISMS”. This project is accomplished under the guidance of our
biology Teacher Mrs. Sunita Mehta. I would like to thank her for all the
lectures, for giving us a bright opportunity and for inspiring us to
complete our project. I sincerely declare that the project made by me
has been carried out on my own initiative and has not been duplicated
or copied from any other source.
Thanking you,

Name: Bhuvansh D. Gorghate

 Acknowledgement
This project is an acknowledgement to the intensity drive and
competence of the individuals who have contributed to it.
The project ‘Effect of antibiotics on micro organisms’ is an
effect of teamwork. It involves the co-ordination of many people
around me. The project was a challenging task for me.
The expert guidance of our biology teacher Mrs. Sunita Mehta
changed the complexity of our project into a perfect and smooth
result. We also give special thanks to her for her suggestion and
inspiration.
I am thankful to our Principal Fr. Binoy, Carmel Academy,
Chandrapur, for enlightening us and for providing us the lab
facility for the accomplishment of the project.
The Acknowledgement would be incomplete without rendering
impartial gratitude to all those who directly or indirectly helped
me in the completion of my project.
Thanking you,

Mas. Bhuvansh D. Gorghate

 INDEX
1. Introduction
2. Aim
3. Requirements
4. Procedure
5. Observation
6. Conclusion
7. Bibliography
 INTRODUCTION
1. Antibiotic - An antibiotic is an agent that
either kills or inhibits the growth of a
microorganism. The term antibiotic was first
used in 1942 by Selman Waksman and his
collaborators in journal articles to describe any
substance produced by a microorganism that is
antagonistic to the growth of other
microorganisms in high dilution. This definition
excluded substances that kill bacteria but that
are not produced by microorganisms (such as
gastric juices and hydrogen peroxide). It also
excluded synthetic antibacterial compounds
such as the sulfonamides. Many antibacterial
compounds are relatively small molecules with
a molecular weight of less than 2000 atomic
mass units.
2. Importance of study of antibiotics - Antibiotic
resistance is becoming more and more
common. Antibiotics and antimicrobial agents
are drugs or chemicals that are used to kill or
hinder the growth of bacteria, viruses, and
other microbes. Due to the prevalent use of
antibiotics, resistant strains of bacteria are
becoming much more difficult to treat. These
represent a threat to public health since they
are resistant to most commonly used
antibiotics. Bacteria are adapting to these
antibiotics making them ineffective means for
treating these types of infection. For example,
Staphylococcus aureus have developed a single
DNA mutation that alters the organism's cell
wall. This gives them the ability to withstand
antibiotic cell disruption processes
Aim – To Study the Effects of
Antibiotics on Micro Organisms

Requirements
Potato ,agar, dextrose , distilled water ,
four different types of antibiotics (such
as penicillin , streptomycin ,
aureomycin , tetramycin ) , syringe ,
oven sterilized petri dish , flasks ,
beakers , pipettes , garden soil , glass
marker pen , etc.
PROCEDURE

A. Preparation of culture Medium -

1. Potato Dextrose Agar (PDA) Medium. Take
200 g of peeled potato chips. Boil them with
500 ml of water in a beaker for 15 minutes.
Squeeze the potato pulp thus obtained through
a muslin cloth and keep it in a flask. Take 20 g
of agar in a beaker and warm it with 500 ml of
water .Mix both the solution of potato and agar
and add 20 g dextrose to it. Thus one liter of
PDA medium is prepared.
Autoclave the medium at 15 pounds pressure
for 15 minutes.
B. Effects of antibiotics on microorganisms in
soil -
i) Take 2 g of soil and dissolve it in 10 ml of
water in a beaker. Let the soil particle settle
down.
ii) Take 5 oven sterilized petri dishes and pour 1
ml of soil suspension in each of the plates. Now
pour 1ml of the four antibiotics separately in
four petri dishes with the help of syringe, and
mark them with marker pen. Leave the fifth
petri dish without antibiotic to serve as control.
iii) Pour PDA in each of the petri dish and mix
the suspension by rotating the petri dish. Leave
the petri dishes undisturbed at a warm place
for an hour.
Iv) Observe the step by step changes and make
note of it .
Observation
Colonies of micro- organisms appear on the
culture medium, count the colonies in each
petri dishes and present your observation in
the following table:

Sr.No. Antibiotics No. of

colonies in a
petridish
1. Penicillin NIL

2. Streptomycin 2-3

3. Tetramycin NIL

4. Chloromycetin 1-2

5. No antibiotic 30-40
The effect of different antibiotics on the
microorganisms can be assessed by counting
the number and size of the colonies growing in
the petri dishes.

Conclusion
Penicillin and Tetramycin were most effective
antibiotics against microorganisms in soil.
Bibliography –
1. Google
2. Wikipedia
3. Encyclopedia
4. Textbook
5. Brainly.com