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Republic of the Philippines

Department of Education
Region X – Northern Mindanao
Schools Division of Cagayan de Oro City
CAGAYAN DE ORO NATIONAL HIGH SCHOOL-JUNIOR HIGH
28th St., Nazareth, Cagayan de Oro City

LEARNING ACTIVITY SHEET (LAS)

GRADE 7

Name: Date: Score:

Subject : SCIENCE 7
Lesson Title : MICROSCOPY: Types of Microscope
Learning Competency : Identify parts of the microscope and their functions
Reference: : Science 7 Learner’s Material; https://www.khanacademy.org/science/high- LAS No.: 2.2
school-biology/hs-cells/hs-introduction-to-cells/a/microscopy

Concept Notes:
Comparing Light and Electron Microscopes

Electron microscopes are powerful microscopes that operate by bombarding a specimen with a beam of
electrons. There are various types of electron microscopes, which apply electrons to the specimen in
different ways. Study the table below to identify the key differences between light microscopes and
electron microscopes.
Feature Light Microscope Electron Microscope
Size Small Very large
Cost Inexpensive ($150 +) Very expensive ($20,000 +)
Training required Little; appropriate for middle school Extensive training required: only laboratory
students and older personnel in research institutions will have
access.
Viewing live Live specimens can be viewed. Live specimens cannot be viewed.
specimens
Can see in color Yes, because light is used to No, because electrons are used to generate
illuminate the specimen. an image. Any colors seen are computer-
generated for clarity
Total magnification 1000X maximum up to around 10,000,000X (some can view
atoms)
Clarity/resolution Vague; generally poor resolution Very clear; very high resolution and clarity.
Types of Electron Microscope
TRANSMISSION ELECTRON MICROSCOPE (TEM) – image is derived from beams of electrons that have
passed through the specimen, most powerful magnification, higher resolution, this higher magnification, used on
seeing the internal structure of something, can only see 2 dimensional images
SCANNING ELECTRON MICROSCOPY (SEM) - a beam of electrons moves back and forth across the surface
of a cell or tissue, creating a detailed image of the 3D surface.
STEREOMICROSCOPE (Dissecting Microscope) - used to observe a larger, thicker, often opaque object. A
light source illuminates the object from above and a second light source illuminates the object from below. The
magnifying power of a stereomicroscope is much less than that of a compound light microscope; objects are
only magnified 10-50 diameters. These microscopes are useful in viewing the external structures of a leaf, an
insect, or a specimen being used in dissection, for example.

Exercises: Choose which type of microscope (stereomicroscope, electron microscope, or compound light
microscope) would BEST be used in the following situations? Justify your answer.
a. A high school student wishes to view his frog dissection a little more closely.

b. A scientist is studying the mitochondria within plant cells.

c. A person is viewing a thin slice of muscle tissue cells.

d. A high school student wishes to view living euglena, protists found in pond water.