Name: Natalie Cole (SJ18172294)

Grade: 3C

Course: Science Concentration 1

Course code: SC321PRB

Lecturer: Mrs. Thomas

Transpiration Lab
Aim: To demonstrate transpiration from the surface of a leaf.
Apparatus: 4 similar leaves, string, Vaseline.
Drawing:

Method:

1) Four (4) leaves that approximately the same size and shape were picked. The leaves were
labelled A, B, C and D. the texture and the colour was observed.
2) Vaseline was rubbed on both upper and lower surface of leaf A.
3) Vaseline was rubbed on the upper surface of leaf B.
4) Vaseline was rubbed on the lower surface of leaf C.
5) No Vaseline was applied to leaf D.
6) The leaves were hanged by the stalk and was placed in the sun for two days.
7) Observation was recorded.

Observation:
Four green leaves were taken from the same tree, all four leaves had the same shape, and

approximately the same size.

After the Vaseline was applied to leaf A on both upper and lower surface of the leaf,

after the observation the leaf did not change and was remain as how it was. When the Vaseline

was applied on the upper surface of leaf B. the leaf became wilt or loses water and was
beginning to weaken. For leaf C, the Vaseline was only applied to lower surface, therefore the

leaf took a longer time to change, and the leaf feel a little flimsy. For leaf D, no Vaseline was

applied on the surfaces, therefore the leaf became wilted that it dried up so quickly.

Discussion:
Transpiration is the release of water from plant leaves. The water eventually is released to the

atmosphere as vapor through the plant's stomata which is tiny, closeable, pore-like structures on

the surfaces of leaves. Transpiration rates vary widely depending on weather conditions, such as

temperature, humidity, sunlight availability and intensity, precipitation, soil type and saturation,

wind, and land slope. Transpiration also helps with the cooling down of the plant. For leaf A, the

Vaseline was applied on both upper and lower surface of the leaf and the leaf remains the same

and did not changed. The Vaseline on the leaf protected the leaf from changing, with this on the

leaf stomata gets covered and transpiration stops. This prevents the loss of water vapour and the

moisture of the leaf retain for sometimes.

For leaf B that the Vaseline was applied on the upper, the leaf began to

wilt. When Vaseline was applied to the upper surface the leaf. The stomatal pore was blocked,

the plant was no longer as healthy as before and little transpiration in the leaf was able to take

place but was ceased and the leaf became weak as the days go. Leaf C, the Vaseline was applied

to the lower surface. The stomata are mostly found on the under surface of plant leaves. There

was not much transpiration taken place as the Vaseline blocks the stomata, so that water cannot

escape from the leaf. The rate of transpiration for plant C was slow. For leave D no Vaseline was

applied to the surface. The leaf became so wilt it started to dried up so quickly within two days.

Transpiration was shown in leaf D as the sunlight open the stomata and because no Vaseline was
applied to affect the rate of transpiration, the leaf dried up. The decrease of mass percentage was

higher in leaf D.

Conclusion: Transpiration was shown on the surfaces of the leaves by applying Vaseline.