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Orcehori.

dreckoma
apt 7 19BBTou7

Sothn Hbidqakion
in:oPrpos Soudhern bloimg DwA hybrid pdiow do
deleet aget DNA

incple Southern blotting coe nare eflor Eoesurd M.Soothern


Cho develope) hispocedure
nin he
at
Echnborgh Unircrsiky
the/970. To orcy Simp DA moleoles Gre
trantferrcd om an aerose gd ondo e membone Sork%er
Hoing desiqncd J
Cocek a pavicob Seqvence d
wA eoihin a
Complea mitore For eccarnple,
Bl0hing oold
Soold be used o Coccde Souther
coithin AN
entire genome paricolau
a

gene
The amoun 7 oA necded Jo ho
cepencant on he eehni
Short Pobcs nd Siye and
Seelre achiviky he pope
move
Condond jou c a r Speciie Unde phional
A o r eshich tapeet 4
gou orc Pobin9 delect
Olp1 4 he
MaLerie A mokers, Beohinqlales probe,
44bridi4okon bulle wes pehybridikiom bef,
Shrepavidin HRP Co)ak, bellc, blockimg le,
Jubara t, tlecko coT eak Cilohion
seen 20, ansfe bl/r, byfe,
membran pebi gar 0se 7AE bloclaing Pocader,
beffe,
dish, Ehidivn ller pecpe-, Nylo
method: bomide, Dishle d
2o ul A
coake
. nmaker
Electaoppor esis C Cs loc,ded onto
1.07.
reached bodtonncanie)
a
oo a
Bo
Bo-(oov anavase
33 Assemble hc -(oov onhl
onil hethe
.
Place he lecherarfe apparaus
Ix elechodcavete inhe
in he
appar atos
anefev belfc Cpparaos lled
Jlled cdh
c>Wh 250mL
?50ml
6.
6 leetrophoresis
Remove h
ess Cawicd o adBov fo 3.5h
1 Remor he awete

.
or 20 min glon ermbranc, and ace h 0v
hayillonmina tov
ncbale i dor 30 mina 70 c
Ge+ loml
prehybridiyohi
Shokev incohalor belfer c inewcwbabe od 45c
10. he
Prehqbrid aro L5mi
hybidiyohiow boll
bol|cc Lscra
Lsco
boe addcd isccrde
dsccvded
s rnd
aud lOml
lOnml
add probcs soloiow addc
12.
Incoba be a 45°e cvith
The milshekinq or 16
hprid yehow bulle c
bull A discardes
Stivlcd Cseadded
bminotes and *he and lom! X
Pebi dis
pehi Sm
om
ces
I. Abood Foonn qenh
qen
1Oml
added and h IK
Prcesnrme (70'C) ses
pehi dis
emparadre
5.
Inwbk
6. 10 at o'e gonhy ssirled byffe B
ml ] bmim
incobakð Conegrh
and
at zoowbelle CsC added
7.The ll s to th
C adècd
p.
discur o /h iHpehidis
smins
Gndhe disdcd Cnd enAe kockina
Jee rockina.
Csidh incutaded at
ixubld
18 cn tle
byfer C coc
ciscaded tcckin oon tmpavatur e
ockin 9.
inwhabe ab v.d cnd
ble
5ml 4 Ix fo 5minn O as
eadded
Jndy Ssivledsobshate olubior
oloion Sa
cnd
blue band
a added Cnd h
20 der eloped
Rx
oomemparawre o 5-20mi On
dij
coa
skppe by pecing he
Resolts: membreane in
dio4lle sae
Soubhern hybndiyahio
Senaple DvA Co conduced

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