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BSC Bioanalytical Technique Assignment
BSC Bioanalytical Technique Assignment
APPLICATION OF
RADIOISOTO PES IN
BloLOGICAL AND MEDICAL
SCIENCES
BIOANALYTICAL TE CHN1gUES
BiOANALYTICAL TECHNIGUES-l09C
ASsIGINMENT ON APPLICATION OFf RADIo 1SOTO PES IN
BlOLOGIl CAL AND MeDICAL SCIENCE
Name: G Gioyotmi
Class: 1s yeon Bsc . Blotechnology
Kegi'ste no 23
Depontmen Bfotechrnology
Dy. GRD Coge o Sttene
CONTENT
5 N TOPIC Page No
01
TNTRODUCTION 04 0 5
03 IN VITRO METHODS 08 09
O4 IN VIyo METH ODS
05 PADIOTHE RAPY 12
01
BEPERENCES 14
RADIOISOTOPES IN BIOLOGY AND MEDITNE
INTRODucTioN-
There is a uufde smead interest (n the peaehma appleotion
o e benehts o atomic energy. n vesy longe msasus
tor
Ph's cenhes around the use o adio ts otopes in mad/ti'ns
and btologq-
reach'ons u hhn an
s a cong
equen ta nucleo
ato ric eneroy pf
pecause e rad'ah'ons enmi Hed bya radio lemend
preodu fouzoh'on fhey v e desor ?bad as fontasnq
proolu
Tad'at ons. Because they oe asso clated u h nuele or
Teachons
Rey Ore Sasd to be nucleo aol'ahtoru
wf
w dely wed is otopes used toY lobeing. Traceas used
FoY diognoshe pwposU Os well a tn rescorch on
metaboUc rocesses. When a Tadie achve s otope is ocloud
Medca vacees
Some eemansend to tonuskate in certauin
ponis
body- (odi'ns in urotd phospherow in bon and
pot osstum in muscle,
races ame
appUed io a
no.o tess, Such as Te
in autoradiographu ond nucle msdietns fncuolinq Singu
photon em/sston Computd tomographyl SpeCT), PET and
Sdntigraphy. Urea bvecoth tesB hell o bocta py lef
Py lerf
Commony used as a doe 4C labelld rea to datat h.
I P4keri tnPech'on. 1F labeled wrea wa
matobolod byh
pylei in stomach , Hho
patens bve ah wo uld Con tain
obe d Carben olioxtd. tn ve cent
yeonsthe we
o
Substanw emichud in ha hon Tocdo a chve fsotope cBC
has beomee Re me Pered he
thodavofd'ng potfent
exprvose to radlieach'r ity
e
radioack'vlty measwad in shtl oled Co untng odevio.
a
Exhra Poios -
Outstan
andlng ex ampla o ths method a
C Use ofrerganic radiotooine tn
tRe study olodhu
uptaka tn ke Reeld gland and in
locaknq tRose
metastases Cotcinoma
Re Hyreld hat readily
take up to dêns an d
C)Use o dlto dofluro uin in Lorahhg Some brain tumoTs.
Ohea examples the se radfoso dtum in u
ae
ol Stuoly
pephen al Vasculas olfsease Ond R se c Eagged
1oolinaed rotein in blood velun and efr cul atron
time studtes.
Diagnosh'e prow olure could be
peafonmud etthea by
n-vivo we eradiop
hanma ultfcals of byin-vitro
use o
rodiof mmuunosay, thu labelling o eay
Can be
iwoyto
achiev ed by In vivo Metiods when Fe
inected intra ve
rnously into normal and anemle animals
2
C4) Radt otherapy
A thesapq
A theraP
using onfaatfon Radiatfen, generaly
rowolad as pagt o Caunces treadmend to Conrol o
ktl mal'gn ant ceu ass nom
ally dullveud bya
ne a9 acalaatoa. f s also Calle d as radtatlon
hadlo Audographs-
These ase made by platkng Scchfonu o Ha
Speuimento be Studted Og ainst a Sensi Hve photogropkie
lm and pemitnq 1 to main t aip Hhs Contoct untila
Subftren Hme ha elapsed For o take lh oun
pfetuse. Beka emi Heas usually qive be Hea esuws han
gamma emlHers. By hus me HRod he topoqraph
okshi buhon h e radio elemant ufhtn he speiman
s readi y dlemonsrated in raphe foshlon. Quan Khaiv
me aguAemenk Yadio oc Hvfty O hot
usually made
bq me Hhod.
Examples aHus techn'au Oe demonsrahon a
radfotoo'n tn excs ed huro td Rssuu, orao'ophosphoreu