ACTION OF

CATALASE ON
HYDROGEN
Chief Squad
PEROXIDE
Baraquil, Ruchie Ann P.
Calderon, Jethro
Dichos, Kaye
Montayre, Ma. Kimberly
Sevilla, Britz Aeron
 Procedure:
Note: Use only an enough portion of potato for the experiment.
2. Peel the potato. Cut, into cubes, equally on your desired size.
Material: Preliminary experiment:
▪ 60mL Hydrogen peroxide or agua oxygenada 3% solution 3. On a separate glass, place one (1) cube each of the potato.
▪ 1 piece of potato (or liver from chicken, pork or beef if available), use only enough 4. Add separately 5 drops of water and 5 drops of hydrogen peroxide in the glasses
▪ Acid: 20mL Vinegar with potato cube.
▪ Base: 1 tablespoon Baking powder (or chopped to fine powder bath soap, except dove soap or 5. Observe if the results.
kojic acid soap or Main experiment:
any soap that is not acidic). Note: check pH level of the soap you are using to confirm pH level as 6. Separately mash five (5) cubes to create a fine substance and place it in the
basic assigned glass container.
▪ 20mL Tap, Cold and Hot water 7. Place the following materials in the assigned glass.
▪ 5 clear glasses with the same size (preferably taller glass or long neck narrow glass) that will ▪ Glass #1: acidic solution (ex. Vinegar)
serve as your 100mL ▪ Glass #2: one (1) tablespoon of the basic powder (ex. Baking powder)
graduated cylinder or 300mL beaker. Hence, please mark the glasses with uniform calibration if ▪ Glass #3: cold water
possible. ▪ Glass #4: hot water
Label the glasses with: Acid, Base, Hot water, Cold water, Tap (neutral) ▪ Glass #5: tap water (room temp.)
But make a separate 2 empty glasses for the preliminary experiment. One for water (in room 8. Leave for 3 minutes. Drain only the acid, cold, hot and tap water after.
temperature) and one for 9. Add 10mL of hydrogen peroxide in each of the glasses.
hydrogen peroxide. 10. Observe the results and compare. You may use calibration as one way to present
▪ Kitchen knife for chopping of the potato (you might include peeler for the removal of its skin) your observations.
▪ Chopping board
▪ Mortar and pestle (food processor or blender, if available)
▪ Medicine cup, syringe, or calibrated dropper for measuring volumes of hydrogen peroxide and
other solutions
SCHEMATIC
DIAGRAM
Peel the potato. Cut, into
cubes, equally on your
desired size
On a separate glass, place
one (1) cube each of the
potato.

. Separately mash five (5)

cubes to create a fine
Add separately 5 drops of water substance and place it in the
and 5 drops of hydrogen peroxide assigned glass container.
in the glasses with potato cube..

Place the following

Observe the results. materials in the assigned
glass.

Leave for 3 minutes.

▪ Glass #1: acidic solution (ex. Vinegar) Drain only the acid, cold,
▪ Glass #2: one (1) tablespoon of the basic powder hot and tap water after.
(ex. baking powder)
▪ Glass #3: cold water
▪ Glass #4: hot water
▪ Glass #5: tap water (room temp.) Add 10mL of hydrogen
peroxide in each of the
glasses.

Observe the results and

compare. You may use
calibration as one way to
present your