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LORIEGA, FAYEANN BSN-2D

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I. Basic tools used in a Microbiology laboratory.

HAIR NET

SURGICAL MASK

ISOLATION GOWN

DISPOSABLE GLOVES

SHOES COVER
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II. Basic tools used in a Microbiology laboratory

Name Photo Function
1. Inoculating loop . The looped end is useful for obtaining
bacterial samples from colonies growing on
media plates or from liquid media, as the loop
can hold a drop of liquid, somewhat like a
bubble wand holds liquid soap.

2. Petri dish s use to hold growth medium in which cells can

be cultured, originally, cells of bacteria, fungi
and small mosses.

3. Graduated cylinder used to measure the volume of a liquid.

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4. Beaker useful as a reaction container or to

hold liquid or solid samples.

5. Alcohol lamp used for heating, sterilization, and combustion

in a laboratory.

6. Pipette used in chemistry, biology and medicine to

transport a measured volume of liquid, often as
a media dispenser.

7. Aspirator for aspirating fluid from a vessel or cavity.

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8. Micropipette to transfer small quantities of liquid

9. Test tube used to hold small amounts of material for

laboratory testing or experiments.

10. Test tube rack used to hold upright multiple test tubes at the
same time.
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11. Erlenmeyer Flask used to contain liquids and for

mixing, heating, cooling,
incubation, filtration, storage, and
other liquid-handling processes.

12. Glass slides used to hold objects for examination under a

microscope.

13. Cover Slip it protects the microscope and

prevents the slide from drying out
when it's being examined.

14. Dropper To transfer small quantities of

liquids

15. Mortar and pestle to prepare ingredients or substances by

crushing and grinding them into a fine paste
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16. Autoclave tape used in autoclaving (heating under high

pressure with steam to sterilise) to indicate
whether a specific temperature has been
reached.

17. Parafilm used for sealing and protecting

vessels such as test tubes, cuvettes,
and petri dishes.

18. Cotton applicator used to clean the area

surrounding a wound, cleaning the
external ear

19. Autoclavable to prevent sheets from sticking

cellophane and to reduce contamination of
sheets while opening the
container.
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20. Vials used especially for holding liquid medicines.

21. Thermometer device that measures temperature or a

temperature gradient.

III. Basic equipment used in a Microbiology laboratory

Name Photo Function
1. Autoclave machine that uses steam under
pressure to kill harmful bacteria,
viruses, fungi, and spores on items
that are placed inside a pressure
vessel.
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2. Laminar flow hood to prevent contamination of

semiconductor wafers, biological
samples, or any particle sensitive

3. Biosafety cabinet n enclosed, ventilated laboratory

workspace for safely working with
materials.

4. Drying oven used for simultaneously heating and

drying samples. Features include
gravity or mechanical (forced air)
convection, capacity, achievable
temperature, programmability, and
scheduled on/off cycles. Application

5. Microwave oven an electric oven that heats and cooks

food by exposing it to
electromagnetic radiation in the
microwave frequency range.

6. Biofreezer/Ultra-low to store drugs, tissue

freezer samples, enzymes, and
other biological materials.
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7. Incubator apparatus providing a controlled

environment for the care and
protection of premature or unusually
small babies.

8. Chiller analytical instrumentation,

equipment cooling, MRI
machines, CT scanners,
Blood Cooling Systems,
Biograph Systems,
Radiation Therapy
Machines, Linear
Accelerator and Lasers
among others.

9. Fume hood device that is designed to limit

exposure to hazardous or toxic
fumes, vapors or dusts.

10. Spectrophotometer for measuring the intensity of light in

a part of the spectrum, especially as
transmitted or emitted by particular
substances.
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11. pH meter pH meter will automatically

recognize each digital electrode,
providing sensor type, calibration
data, and serial number.

12. Mechanical shaker/ for culturing microbes,

Orbital shaker washing blots, and general
mixing.

13. Vortex mixer to mix small vials of liquid. It consists

of an electric motor with the drive
shaft oriented vertically

14. Digital water bath for maintaining temperature set

points over a long period of time.
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15. Tissue ideal for mopping up

liquids and hand wiping.

16. Rags used for cleaning, washing,

IV. Basic chemicals and media used in a Microbiology laboratory

Name Photo Function
1. Brilliant Green Bile Brilliant Green Bile Agar is
Agar recommended for
enumeration of coliform
bacteria in water and
wastewater.
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2. Nutrient Agar (NA) NA is commonly used to

grow different strains of
fungi and bacteria. It
supports the growth of
wide range of non-
fastidious microorganisms.

3. Nutrient Broth (NB) employed for non-

fastidious organism culture
and maintenance, as well as
enumeration of organisms
in water, sewage, dairy
products, feces, and other
materials
4. Brilliant Green Bile Formulated for coliform
(BGB) Broth bacteria presumptive
identification and
confirmation. Gram-positive
bacteria, such as lactose
producing Clostridia, are
inhibited by ox-bile and
brilliant green.

5. Brilliant Green Agar selective and

differential medium for
the isolation of
Salmonella species
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6. MacConkey Agar It is designed to

selectively isolate Gram-
negative and enteric
(normally found in the
intestinal tract) bacteria
and differentiate them
based on lactose
fermentation.

7. Agar agar agar-agar, is widely

used as a culture
medium for growing
micro-organisms. The
stuff itself is also the
product of micro-
organisms.

8. Blood agar used to grow

fastidious organisms
and to differentiate
bacteria based on their
hemolytic properties.

9. Thiosulfate Citrate used in microbiology

Bile Salts Sucrose laboratories to isolate
(TCBS) Agar Vibrio species. TCBS
agar is highly selective
for the isolation of V.
cholerae and V.
parahaemolyticus as
well as other Vibrio
species.
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10. Ethyl Alcohol 70% This potent 70% denatured

alcohol solution can be
used to disinfect as well as
clean and dry the skin.
Ethanol is a common
element in many cosmetics
and beauty products
because it kills bacteria,
fungus, and viruses.
11. Denatured Alcohol It can be used as a cleaning
agent, a fuel additive, a
sanding aid, a pesticide,
and a solvent. A wide range
of additives can be
employed, with 10%
methanol being a popular
choice.

12. Disinfectant spray On still surfaces,

(e.g. Lysol) biochemical instruments
are used to kill
microorganisms such as
bacteria and germs. They're
commonly used to
eradicate bacteria and
stains in hospitals, dental
offices, kitchens, and
restrooms.