Hariri High School II

Biology Worksheet

Name: _______________                                                                                  Date       : Dec. 2021

Class: Grade 9 (A & B)                                                                                     Sore : /10

Exercise I Role of Enzymes in Digestion (2013 – session 2)

To verify that enzymes are necessary in the chemical digestion of food, a student realizes the following
experiment.

 He puts 5 g of cooked starch in each of two test tubes A and B.

 He adds 1 mL of distilled water to each of the two tubes A and B.
 He adds salivary amylase to tube B.
 Then, he places the two tubes A and B in a water bath at a temperature of 37°C.

A. Schematize the steps of this

experiment by using the
legends in the document to
the right.

B. Pick out the hypothesis

tested in this experiment.

After 20 minutes, he adds to the contents of both tubes several drops of iodine water having brown
orange color. The content of tube A becomes dark blue in color while the content in tube B remains
brown orange.

C. Indicate the tube in which digestion of cooked starch takes place. Justify the answer.
D. Name the substance resulting from the complete digestion of starch.
E. Name the test that permits to verify the presence of the substance resulting from this digestion.
Exercise II Bromeline (2016 - session 1)

Pineapple juice contains the protease Bromeline. To verify if this enzyme has the same efficiency as
the proteases secreted by the pancreas, the following experiment is performed.
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Three test tubes A, B and C are placed in a water bath at 37 C for a convenient period of time. The
tubes contain:

Tube A: water + chopped coagulated albumin

Tube B: water + chopped coagulated albumin + Pineapple juice
Tube C: water + chopped coagulated albumin + Pancreatic juice

At the beginning of the experiment, the aspect of the three tubes is turbid. At the end of the experiment,
the aspect of tube A stays turbid, but the aspect of tubes B and C becomes clear.

A. Name the protease present in the pineapple juice and a protease present in the pancreatic juice.
B. Pose the problem at the origin of this performed experiment.
C. Interpret the obtained results.

At the end of the experiment, the quantity of amino acids measured in both tubes B and C is the same.

D. Show that Bromeline and pancreatic protease have the same efficiency.

Exercise III Digestion of lipids (2013 – session 1)

In the framework of studying the importance of

mechanical digestion, we realize an experiment on in
vitro digestion of lipids: sheep fat. The conditions of
this experiment are shown in document 1.
The mass of sheep fat is measured at he beginning of
the experiment and at the end of the experiment. The
results are shown in document 2.

A. Draw a histogram (bar graph) showing the variation

of the mass of sheep fat in each of the tubes A and
B at the beginning and at the end of the experiment.

B. Determine the effect of mechanical

digestion on chemical digestion.

C. Name the products obtained at the end

of the complete digestion of lipids.
Exercise IV Intestinal functions (2017 – session 1 modified)

In the framework of studying one of the

functions of the small intestine, the
quantity of nutrients is measured in this
organ as well as in the blood leaving the
small intestine after a meal. The results
of the performed measurements are
represented in the document to the right.

A. Pose the problem studied in the

above experiment.
B. Identify the intestinal function
revealed in the document?
C. Indicate another function ensured by
the small intestine.
D. List the characteristics of the surface
of exchange of the small intestine.

Exercise V Celiac Disease (2019 – session 1)

The intestinal villi are small finger-like projections that line up the inner intestinal walls of the small
intestine and increase considerably its surface area. Certain individuals who are affected by celiac
disease show symptoms such as fatigue, anemia and diarrhea upon eating food diet rich in gluten.
The document below shows the difference in the inner walls of the small intestine of a normal
individual and of an individual affected by celiac disease.

A.

1. Indicate the role of the intestinal villi.

2. List the three characteristics of the intestinal wall which favor this role.

B.
1. Compare the aspect of the inner wall of the small intestine in these two individuals.
2. Draw out the characteristic(s) of the inner wall in the individual affected by this disease.

C. Justify why this disease can slow down the growth of the infant although he consumes a balanced
food diet.