DNA FINGER

PRINTING

ISHITA BISWAS
 ARMY PUBLIC SCHOOL
BARRACKPORE

BIOLOGY INVESTIGATORY
PROJECT

NAME –ISHITA BISWAS

CLASS-12
BOARD ROLL NO.-
SESSION-2022-2023
 Self Certificate
This is to certify that I, Ishita Biswas of
class XII F of ARMY PUBLIC SCHOOL,
BARRACKPORE have solely and
exclusively done this investigatory project
pursuing essential vital assistance from
my teacher and others having knowledge
concerning the subject matter. I have also
taken references from the web in due
course to formulate a successful project.
The basic of the project has been put
together on bonfire grounds and the allied
information is truly genuine.
 Certificate
This is here to inform that Ishita Biswas
student of class XII F has successfully
completed the project titled "DNA Finger
Printing" under the guidance of Mr.
Mainak Sengupta (subject teacher
biology) during the year 2022-23 in
fulfillment of the biology practical
examination conducted by AISSCE, New
Delhi.
 Acknowledgement
In the accomplishment of this project successfully,
many people have owned upon me, their blessings
and the heart pledged support, this time I am
utilizing to thank all these people, those who have
been concerned with this project.

I would like to thank our principal Mrs. Moitreyee

Mukherjee and biology teacher Mr. Mainak
Sengupta whose valuable guidance has been one
that helped me patch this project and make it full
proof success, his suggestions has served as the
major contributor towards the completion of this
project.

Lastly, I would like to place my sincere to all who

helped me directly or indirectly to complete this
project on the time to my family whose appreciation
and motivation persuaded to give my best.
 DNA Fingerprinting
DNA fingerprinting is a laboratory technique used to
determine a probable identity of a person based on the
nucleotide sequences of certain regions of human DNA
that are unique to individuals.

The technique was developed in 1984 by British

geneticist Alec Jeffreys, after he noticed that certain
sequences of highly variable DNA (known as
minisatellites), which do not contribute to the functions
of genes, are repeated within genes. Jeffreys recognized
that each individual has a unique pattern of
minisatellites (the only exceptions being multiple
individuals from a single zygote, such as identical
twins). The procedure for creating a DNA
fingerprint consists of first obtaining a sample of cells,
such as skin, hair, or blood cells, which contain DNA.

In Jeffreys’s original approach, which was based

on restriction fragment length polymorphism (RFLP)
 technology, the DNA was then cut at specific points
along the strand with proteins known as restriction
enzymes.

DNA fingerprinting steps:

Isolating the DNA.
↓
Digesting the DNA with the help of restriction endonuclease
enzymes.
↓
Separating the digested fragments as per the fragment size by
the process of electrophoresis.
↓
Blotting the separated fragments onto synthetic membranes
like nylon.
↓
Hybridizing the fragments using labeled VNTR probes.
↓
Analyzing the hybrid fragments using autoradiography.
Case Study