Schematic Diagram: Biochemical Tests for Lipids (Qualitative Tests)

A.

THE There willl be

GREASE Drop a small amout
of oil on a piece of
observed
translucence due to
SPOT paper. the presence of
lipids.
TEST

B.

TEST Add one or two

FOR Take a few drops
drops of very
dilute alkali
The color will
disappear as the
FREE of phenolphthalein
solution in a test
solution, just
efficient enough to
Add a few drops
of oil and shake.
alkali is
neutralized by the
FATTY tube.
give the solution a
pink color.
free fatty acids
present in the oil.
ACIDS

C. (theoretical; reagent unavailable)

 in one test To each tube,
tube, add 2 add 2 drops Large fat
EMULSI Take two ml water and of corn oil
Allow the
droplets are
tubes to stand
FI- clean and dry in the other, and shake
for two
emulsified
test tubes. add 2 ml vigorously into smaller
CATION dilute bile for one
minutes.
ones.
solution. minute.

D.

Mix them thoroughly

Take 1 ml of the oil
and keep the mixture
SAPONIFI- in a test tube and add There will be froth
during the course of
an equal amount of formation in the
CATION alcoholic KOH
warnming and shake
presence of lipids.
up gently with a little
solution
distilled water.

E.
 Take pure glycerol
TEST FOR in a dry test tube;
Warm gently t mix There would be a
add to it a few
GLYCERO crystals of
and then heat presence of a
strongly. pungent smell.
L potassium
hydrogen sulphate.