Objective

Studying (a) binary jission in Amoeha, and (b) budding in veast and Hydra with the help of prepareu stued

1.

Materials Required
1 Prepared/permanent slides of Amoeba. In case of non-availability of any permanent slide, collect
stream water in a beaker.
some pond/
2. Compound microscope.

Procedure
study
the cover slip. the binary fission in Amoeba, take a small drop of pond/stream water on a plain slide and cover it with
Do not
ot add any stain. Put the slide on the stage of the microscope.

ethe permanent slide is available, the slide the stage of microscope.

place on

e mirror of microscope so that maximum light is reflected from the mirror to the slide. Adjust the eye-
by us nicroscope so that the slide is clearly focussed. Observe the slide at first under low power of microscope
Coarse adjustment. Then observe it under high power by using fine adjustment.

97
Observations DSSOT soneSee
sute ai b m a
be in e
. Amoeba are usually iregular in shape. Some of them mav
inesis may be seen. imto ya
.
So that
2.
Others may show the division of cel body, i e cmokinesis.

daughter cells

Dwicg Tuoes

- FEUIDDDde

Darent
ctio

stages of binary fissio

in AmDebE

Conclusio
Gven slide shows he elongated bov of 4moeha wItt E conSICTiOT n he md s a R
under the process of division. hence the grnen slide shows hinar issim m m

B. BLDDING IN YEAST AND TR4

Materials Required
1. Prepared permanent slides showing budding in yeast and ä r
A culture of baker's yeast may also be prepared in the unáementioned way
(a) Add 5 g of yeast powder 1o about 250 ml of 10 sugar solution a sk , dIssohr s
in 250 ml of water
(b) The mouth of the fask shouid be tghtihy corked
(c) Keep the fask in a warm place for about 4 duys
2. Compound mcroscope.
Procedure
1. To study budding in yeast oells takea saa dp ot the culture solution on plan shar ar
coverslip. Do not add any staIn Pui the shc on the sage the micTOSOne
In case the permanent slhde is avalahie piace the sae an the sage of the
macr e
Adjust the miror of microscoe o that maumun ght s ehecied mam he m the s
Adjust the objective lens
of the meroscone s hat shie is
cicarty iacussed
2.Place the permanent shde of hra on he sage o e miTNOE End
power, then under high power of mmcrosoope
osenve carciul

Observations
In Yeast
1. Yeast cells are usualy sphenical or onal in
shan
2. Yeast cells show pronaberances on them caled bads.
These bu nm
parent cel1. This process of repradxtt s knoan as beddire rachg t
3. Sometimes a chain of buds is seen on the
parent e

D e

Budding in Yenst
 Hydra i6 &ultiocilular crgatis hat contains ogemenatie oells apmeas m
A bulging ateral cungw
This outgrowh is a developing bud
2 The bud enlarges in siz and amaits he
shae of the parent bdy
3. Now the fulily matre bud detaches fron he
parent body and becomesa nu du

Parent ydra

Deacmed d
Budding in Hydra