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ZMKG TESTS
Cacn o the letles n IMViC" stonds tos o of nal tesa
I J indole ; M" o t muthyt ld y o6, vogqu-
Puoskallue, ano "c io ou cituat ,towucose 4added ou tt
the ead D buonunaabn, tMVit Jo on ou0 num thastand
O o u ukkuent tests
nolble test
Methyt seol tet
V.ogu Loskauue test
. Citcdte utiuzaution test

To olbtain dhe s u l otheal kou tealo,t w test tubu au

inOculated typtone bLottn Cindole test), methyl ned CVoges
PAoskaueu) buott LR-VP buoth) ound cituate
sIMViC testo aue employeol in ut iolenbipccation / olutleuevntiatio
ormermbLA of {amil hteo bacteuauace

INDOLE TEST
nple: Some l6acteua coun puoduuce ndole krone anino acwc
yptbpbnam using u mgyme typto phatcar

ttyptoblaanad
Lnolol + pyuvae aud + NH
yptophan HO
Loduetion o ndol w detected uatna Ehulueh's Aagent ot kovac's
0qnt. mdole. acla wittn alodeyolk in u Aeayent to qut
Xecl colc , d oulcohoue Laye con certates t ed tolo

lmo a u e top

0 cecluu: bacteuium to be tesCca w LnO cullatecl in bebtont wate

amunO oLCica
ac
tYptophan and inculbated ouLnight
whw ch ontauns
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ot 31'c, followng nauubation Lew duos o Kovac's wogent

aUML added Kovat's wagunt condkt ot baud-dimthyt aluuno
oungoldhude, iuoovmyl bWicohol and cone. Hd,Chilech
eagen mou sthaitiv n dutectiua indole puoduction in
Ondalolt and non-eentiis. Foumat ok wd otlaunk colou
cd Aina, ot the o tokun Qo bosibiv

METHYL RED CMR) TEST

PAinoipal Uhio co to dletect tnt abiuby Qan 0ganusm to
bAoduuce and mauntaun stablu aud end
ptboluct om qutOAl
eumentabion fome bactewa buooluct Louaé amownti aleol
fom
Vawcoat lermentoution bnat theu ovwuomuthe bullein
he system. Methyl Red
actioh o
a pk mdicato, whiK sendains ed
n coldum at a L o t. t O Cs

Poceduuwl: Jhe boctenium tested

to bL
unoouwloted unto gluuD4e
phosphote buoth, witkn contoin quucO
ementatio theoet Eeeej
bvtom brt buetna uetto- systen- and a phoapuate
huke and waukateol at 3TC to 4 houws. Ou Bue
4-8 hows
tnwuned - aid puoductna OLgautium must
bodu sulfuient aud
to ouborm the phosplate
louurte and numauw awd he þH of
the meduum d tested l the doli
tion o 5 odobs o MR ALalgent.
DLULlopment of Ad toloik io takun aa
positi MR negabul ouganion
pooluuce yellou olouu

VoGES PROS KAUER (Ve) TEST

Pinaible Whul MR Test u sekuul n oletecti mured aud
buodulcev, VP best detecbs butylenu qlycol podluLces
dutoin
Gucost
KOH biacetyt + H2O
ocetoin
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Dacetyt t quonioune nabhBot

bCnk colow
Component
Obeptoru

cetyt mety L couubunol Cacctoin) u om intwrneduate i n tut

bLoductior
obutulent otu Col. dn this test huo magenb, 4o/ KOH and
thol o adolet to test buoth alte
-nabh-
itubotion ound ensosed to atmos
piheuc oxug. acetoun u pieseht, t o 0idized in
OU ound kOH to
piesenct ok
diacetyl. Diocetyl uun eacu wit quaniolint
cOmbonenb o btptontin besurc o -haphthol toud colou
Rote of naphthol t u a b
o coltalystand a colol intens itie
PAocedue: Bacteuwm to bi testeo is inoculated mbo quLcol hos plhate
baotn ound inecuboted 4o at east 43
howrs. o.6 ml o alpho habhbtnol
w added to tue test buotn ond
shakiun. 0.2ml of 401. KOH added
bo t buoth anol shakin . thu tuwbe allowed 6 stound o 15 mina
bbLouan.ce o ed colDw u taken boutiue test. U negotue
bulbo muuat lbe held o Onu hou,
Atnd manum colou olvelop-
mun ocwhs wuthin Cne hou ae
adlou ton eaqents

CITRAT E UTILL SATION TEST

Puunable: Jhis test detech the abiluy o an
oq anism to ubuze
Ctuotea ttut sole souuce o Caubon ounol enercry Bacteua tut
noculatrd ovnmecuwm contau nuna s0duum cttuate a n a pH
a

indicato bemotuymol blu h e medu unm also Ontanu noiganue

armmonwwm salb, huch utilegeol solk onc
as
onutuogen
cittate Oxaleaeetate + dcetatE
Oxatoacetate Pyuwate + Co2
CO2 + Na t H2 tog COg
UiuzatDn o uhate inuolus t t enzymt uuutase, whih brtaks
olowt cutuate to onaloCcetate and &eetate. Onaloacetout e io fwthn
buoken odlown to lpyuVate ound to2 PLoduchDin O Na, CO, as wel as NH3
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om utiligabio o 40diuum cituatt and amooni um 400 ws

ouls iolkalil pH.hin sults u thang of madums

coLDuAom gueunn to bluuu

PLoceduw: Bacteuio colonit oul pickiod u huom a stuaight

uwL Oun.d inoculauted ünbo slobl D Simnions cLtuate aga

Qund incubobed ouLuight at 37t y tu oganism Kas
abu ut CD ubiua e utuate be mediuim hahe to colou
h o n qceen topluu

IMViC Teat taulo 50m Genno oEnteLObacttuiactal

B.acteum Indole MR VP cibaute

E&cheicluio col

Klebaitllo aunogunu + +

PLDbeus wwlgauw +

Cituo lboutte klewnolui +