Data and Calculations

(𝑚𝑙 𝑜𝑓 𝐹𝐴𝑆 𝑏𝑙𝑎𝑛𝑘 − 𝑚𝑙 𝑜𝑓 𝐹𝐴𝑆 𝑠𝑎𝑚𝑝𝑙𝑒) × 0.1 𝑀 × 8000 × 𝑃

𝐶𝑂𝐷 =
𝑚𝑙 𝑠𝑎𝑚𝑝𝑙𝑒

(19.1 − 11.7) × 0.1 𝑀 × 8000 × 10

𝐶𝑂𝐷 =
2.5 𝑚𝑙

𝐶𝑂𝐷 = 23.680

𝐴 × 𝑁 × 8000
𝐷𝑂 =
𝑚𝑙 𝑠𝑎𝑚𝑝𝑙𝑒

3.5 𝑚𝑙 × 0.025 𝑁 × 8000

𝐷𝑂 =
100

𝐷𝑂 = 7

𝐴 × 𝑁 × 8000
𝐷𝑂 =
𝑚𝑙 𝑠𝑎𝑚𝑝𝑙𝑒

6.1 𝑚𝑙 × 0.025 𝑁 × 8000

𝐷𝑂 =
100

𝐷𝑂 = 12.2

𝐵𝑂𝐷5 = [(𝐴0 − 𝐴5) − (𝐵0 − 𝐵5)] × 10

𝐵𝑂𝐷5 = [(12.2 − 4.2) − (5.6 − 2.1)] × 10

𝐵𝑂𝐷5 = 4.5 × 10

𝐵𝑂𝐷5 = 45