Name:____________Vicente Reiriz del Rincón__________ Date:_________

DNA Replication

Identify the structure

1. ___Helicases_____ Enzyme that unwinds DNA
2. _Okazaki fragments_______ Fragments of copied DNA created on the lagging strand
3. ______Leading Strands____________ The strand that is copied in a continuous way, from the 3’ to 5’ direction
4. _____Ligase___________ Binds Okazaki fragments
5. _________Polymerase__________ Builds a new DNA strand by adding complementary bases
6. ________Helicase____ Stabilizes the DNA molecule during replication
7. ____Leading______________ Strand that is copied discontinuously because it is traveling away from helicase
8. ______Primase_____________ Initiates the synthesis DNA by creating a short RNA segment at replication fork

9. Place the events in the correct order:

_2_ DNA polymerase adds nucleotides in the 5’ to 3’ direction
4__ Replication fork is formed
__3 DNA polymerase attaches to the primer
_1__ Okazaki fragments are bound together by ligase
5__ DNA helicase unwinds DNA

10. Why is replication called “semi-conservative?”

Because each helix that is created contains one strand from the helix from which it was copied.

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DNA Replication - Labeling (with word bank)

DNA polymerase 3’ 5’ DNA Ligase Okazaki fragment DNA Primase

Single Strand Binding Proteins Helicase Leading Strand Lagging Strand

Identify the structure

1. _____________________________ Enzyme that unwinds DNA
2. _____________________________ Fragments of copied DNA created on the lagging strand
3. _____________________________ The strand that is copied in a continuous way, from the 3’ to 5’ direction
4. _____________________________ Binds Okazaki fragments
5. _____________________________ Builds a new DNA strand by adding complementary bases
6. _____________________________ Stabilizes the DNA molecule during replication
7. _____________________________ Strand that is copied discontinuously because it is traveling away from helicase
8. _____________________________Initiates the synthesis DNA by creating a short RNA segment at replication fork

9. Place the events in the correct order:

____ DNA polymerase adds nucleotides in the 5’ to 3’ direction
____ Replication fork is formed
____ DNA polymerase attaches to the primer
____ Okazaki fragments are bound together by ligase
____ DNA helicase unwinds DNA

10. Why is replication called “semi-conservative?” ___________________________________________________

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