LAB REPORT FORM

(Name) Princess Alyssa C. Altarejos (Date) October 04, 2022

Title: Proving the production of sugar and evidence of chlorophyll in

photosynthesis
Purpose/Problem:
- To show that sugar is produced by photosynthesis
Hypothesis:
Null Hypothesis:
- The color of the Mayana leaf will disappear.
Alternative Hypothesis:
- The concentration of carbon dioxide was increased, then the rate at which
photosynthesis occurs will also increase.
Materials/Supplies:

- tincture of iodine - petri dish

- denatured alcohol - tripod
- wire gauge
- water bath - box of matches
- alcohol lamp - fresh leaf of Mayana

- medicine dropper
- beaker

Procedure:

1. Get a coleus leaf (Mayana).

2. Remove the leaf color by boiling it in alcohol. To do this, follow the steps

below:

a) Fill the beaker (3/4 of its capacity) with water. Let it boil. See Figure 3 for the
sample setup.
 b) While waiting for the water to boil, get a leaf sample and place it in a test tube.
Pour denatured alcohol into the test tube until the leaf has been submerged. See
Figure 3 for the sample setup.

c) When the water in the beaker boils, place the test tube with leaf and alcohol
in it. Let the water boil for another three minutes or until all the color of the leaf
has been extracted. See Figure 4 for the sample setup.

Remove the test tube from the beaker. Then, get the leaf out of the test tube.

3. Rinse the leaf with water, and then place it on a petri dish.

4. Put drops of iodine, until the leaf has been soaked.

5. Observe the leaf.

If the color of the leaf turns to bluish black, it indicates the presence of starch. No
change in color indicates absence of starch.

Observations and Data:

The Mayana leaf in the test tube undergoes a rapid color shift from violet to green
when immersed in boiling water. The denatured alcohol helps in extracting
chlorophyll and therefore, helps in changing the violet color of the Mayana leaf to
color green. Due to the denatured alcohol content, it boils rapidly as well. Our
teacher added a few drops of iodine and the leaf became a bluish-black color,
indicating the presence of starch. This demonstrates that the leaf has been
performing photosynthesis and producing starch.

Conclusion/Summary:

This lab (experiment) investigated is all about Investigating the presence of

starch in the Mayana leaf . In order to study the problem we
gather all the materials needed for this study and we follow all the
procedures that are stated in the book_. My results showed
that the color of the Mayana leaf changed from violet to bluish-black, thus
proving my hypothesis was incorrect since, the color of the Mayana leaf did
not disappear instead, it changed from violet to green and when the tincture
of iodine was dropped into the leaf, it turned into bluish-black.
I believe the results are accurate because we have followed all the procedures
correctly and we have properly observed and collected all the data needed in
this study. In order to further investigate this problem, next time I would
research more information regarding this topic _.

LAB REPORT RUBRIC

LAB REPORT ITEMS Points Points

Received
PROBLEM 10

HYPOTHESIS 10
(Independent & dependent variables included)
MATERIALS & PROCEDURE 15
(All steps clearly stated)
OBSERVATIONS AND DATA 20
(Measurement units identified)
GRAPHS AND/OR ILLUSTRATION 20
(Title, axes labeled, data points plotted)
CONCLUSION 15
(Answers the problem, explains results)
NEATNESS 10

TOTAL GRADE 100