Welcome to

Biology Class!

I’m Teacher Vienne

 Genetic
Engineering
 Learning Competencies

At the end of this lesson, you shall be able to:

1. Outline the processes involved in genetic
engineering (STEM_BIO11/12-IIIa-b-6 ); and

2. Discuss the applications of recombinant DNA

(STEM_BIO11/12-IIIa-b-7 )
 Pre-test

Direction: Identify what is being described in the following statements.

__________ 1. It carries the genetic information in all living organisms.
__________ 2. It is a single stranded type of nucleic acids that is mostly
involved in protein synthesis.
__________ 3. It is a protein molecule that catalyzes the biochemical
reaction.
 Pre-test

Direction: Identify what is being described in the following statements.

DNA
__________ 1. It carries the genetic information in all living organisms.
RNA
__________ 2. It is a single stranded type of nucleic acids that is mostly
involved in protein synthesis.
ENZYME 3. It is a protein molecule that catalyzes the biochemical
__________
reaction.
Review
 Genetic Engineering
• the artificial manipulation, modification, and recombination of DNA or
other nucleic acid molecules in order to modify an organism or
population of organisms
 Classical Breeding
• the practice of mating or breeding organisms with desirable traits

Guapple
 Classical Breeding
• the practice of mating or breeding organisms with desirable traits

Siberian
Husky
 Steps in Classical Breeding

1. Determine which traits are significant enough to be chosen.

2. Select parents that exemplify these traits.
3. Choose the best offspring from parents to produce the
next generation.
4. Repeat the process.
 Recombinant DNA Technology
• The modification in genetic engineering may involve the following:

1. introduction of new traits into

an organism,
2. enhancement of a present trait
by increasing the expression of
the desired gene, or
3. enhancement of a present trait
by disrupting the inhibition of
the desired genes’ expression.
 Steps in
Recombinant
DNA
Technology
(Genetic
Engineering)

Source: https://youtu.be/OpU_CQ0pFyQ
 Question
You are working in a molecular biology lab and, unbeknownst to you,
your lab partner left the foreign genomic DNA that you are planning
to clone on the lab bench overnight instead of storing it in the
freezer. As a result, it was degraded by nucleases, but still used in the
experiment. The plasmid, on the other hand, is fine. What results
would you expect from your molecular cloning experiment?
a.There will be no colonies on the bacterial plate.
b.There will be blue colonies only.
c.There will be blue and white colonies.
d.The will be white colonies only.
 Question

If you had a chance to get your genome

sequenced, what are some questions you might
be able to have answered about yourself?
 Activity
Direction: Based on what you have watched, arrange the following steps in
genetic engineering into their correct order, as 1 being the first step and so on.

_____ the plasmid is removed from the bacterium

_____ the DNA fragment containing the gene of interest is inserted into a
plasmid using DNA ligase
_____ the gene of interest in a DNA fragment is obtained from a donor cell
_____ restriction enzyme is used to cut the DNA fragment and the plasmid
 Activity
Direction: Based on what you have watched, arrange the following steps in
genetic engineering into their correct order, as 1 being the first step and so on.

2 the plasmid is removed from the bacterium

_____
_____
4 the DNA fragment containing the gene of interest is inserted into a
plasmid using DNA ligase
_____
1 the gene of interest in a DNA fragment is obtained from a donor cell
3 restriction enzyme is used to cut the DNA fragment and the plasmid
_____
Applications
of
Recombinant
DNA
Source: https://youtu.be/h5oTviGOjRw
 Generalization
Direction: Enumerate the pros and cons of utilization and consumption of
GMOs.

Pros Cons
 Post-test

Direction: Write True if the statement is correct and False if incorrect.

_____ 1. A ring of DNA in a bacterium is called a plasmid.
_____ 2. Genetic engineering techniques cannot be used to make crops
resistant to destructive insects.
_____ 3. No cases of illness have ever been reported by people who have eaten
foods that have been genetically modified.
_____ 4. In the genetic modification process, scientists often use viruses and
bacteria to invade cells of plants and insert foreign genes.
_____ 5. Restriction enzymes are used to cut DNA molecules into pieces.
 Post-test

Direction: Write True if the statement is correct and False if incorrect.

True 1. A ring of DNA in a bacterium is called a plasmid.
_____
False 2. Genetic engineering techniques cannot be used to make crops
_____
resistant to destructive insects.
False 3. No cases of illness have ever been reported by people who have eaten
_____
foods that have been genetically modified.
True 4. In the genetic modification process, scientists often use viruses and
_____
bacteria to invade cells of plants and insert foreign genes.
True 5. Restriction enzymes are used to cut DNA molecules into pieces.
_____
 Integrative
Performance
Task # 1