Bio-Rad CDM System PATIENT REPORT

Bio-Rad Variant V-II Instrument #1 SN 12845 V2_BThal

Patient Data Analysis Data

Sample ID: 88522317 Analysis Performed: 25/11/2016 01:32:51
Patient ID: Injection Number: 12807
Name: Run Number: 575
Physician: Rack ID: 0005
Sex: Tube Number: 10
DOB: Report Generated: 08/05/2017 09:13:13
Comments: Operator ID: ri

Calibrated Retention Peak

Peak Name Area % Area % Time (min) Area
Unknown --- 0.8 0.96 17490
F 0.6 --- 1.04 12443
Unknown --- 1.9 1.18 43692
P2 --- 5.8 1.31 130814
P3 --- 23.6 1.54 533918
Unknown --- 1.1 2.07 25545
Ao --- 64.2 2.36 1451325
A2 1.8* --- 3.55 43779

Total Area: 2,259,005

F Concentration = 0.6 %
A2 Concentration = 1.8* %
*Values outside of expected ranges

Analysis comments:

45.0

37.5
1.54

30.0
-

22.5
- 1.31
%

3.55

15.0
- 1.18
1.04

2.07
-- 0.96

2.36

7.5
-

A2
F

0.0
-

0 1 2 3 4 5 6
Time (min.)
 Library of Variants
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Hb Le Lamentin

Variation data: Alpha2 20(B1) His-Gln

Case commentary:
• 57 year old male, known diabetic, routine HbA1c request.

Chromatography: See attached

P3 Peak of 24% detected

Other methods: See attached

This sample was also tested using the Bio-Rad VARIANT II Beta Thal Short Program.
This showed an Hb variant of 24% with a RT of 1.54min.

Hematology and Clinical Presentation:

Known diabetic, recent iron therapy.

CBC data:

HB 134 g/L
RCC 4.50 x 10^12/L
MCV 89 fL
PCV 0.400
RDW 21 %
MCH 29.6 pg
MCHC 332 g/L

Ethnicity: Unknown

Other studies: Final identification of this sample performed by DNA

Alpha-1 Globin Sequencing
NG_000006.1(HBA1):c.[63C>A, p.His21Gln] ; [=].

DNA sequencing of the alpha-1 globin gene has detected a heterozygous,

single base change (C>A) that causes a change from Histidine to
Glutamine (CAC>CAA) at amino acid 20 (20.3) of the mature protein.
This result is consistent with Hb Le Lamentin.
External links:

Case and Comments provided by:

Chris Smith, Western Diagnostic Pathology, Myaree, Perth, Western Australia

References: http://globin.bx.psu.edu/

Do not change the disclaimer

Disclaimer: The chromatogram and data in this case report are actual laboratory
findings. Bio-Rad Laboratories, Inc. does not validate or confirm the sample data
included in this database. All information contained herein is for informational use only
and is not meant as a definitive identification of the hemoglobin genotype nor for
diagnostic purposes. We welcome your comments at hemoglobins@bio-rad.com