SOP for Operation & Calibration of UV-Vis Spectrophotometer

Objective:

This Standard Operating Procedure (SOP) outlines the procedures for the operation and calibration
of the UV-Vis Spectrophotometer.

Scope:

This SOP applies to the UV-Vis Spectrophotometer used for analytical purposes at [Laboratory
Name/Location].

Equipment:

UV-Vis Spectrophotometer [Specify Model and Make]

Calibration standards

Analytical Balance

Responsibility:

[List Responsible Personnel Names and Roles]

Procedure:

Preparation:

a. Ensure that the UV-Vis Spectrophotometer is clean and free from any residual samples.

b. Power on the spectrophotometer and allow it to warm up for [Specify Warm-Up Time].

c. Verify that the calibration standards are available and suitable for the calibration.

Instrument Calibration:

a. Perform the calibration of the UV-Vis Spectrophotometer following the manufacturer's guidelines.

b. Record calibration details, including date, time, and results, in the calibration log.

Calibration Verification:

a. After calibration, perform a verification test using a standard solution.

b. Compare the test results with expected values or limits.

c. Record the verification results in the calibration log.

Sample Measurement:

a. Prepare the sample following the specified procedure.

b. Place the sample in the cuvette and insert it into the spectrophotometer.

c. Use the appropriate wavelength and settings to measure the sample absorbance.

Data Analysis:

a. Record and analyze the absorbance data obtained.

b. Calculate the concentration or other parameters of the sample as necessary.

Shutdown:

a. After measurement, clean the cuvette and any sample residues.

b. Turn off the UV-Vis Spectrophotometer.

Documentation:

a. Maintain records of instrument calibration, verification, sample measurements, and any

deviations.

b. All records should be signed and dated by authorized personnel.

Maintenance:

a. Regularly maintain the UV-Vis Spectrophotometer as per the manufacturer's recommendations.

b. Address any malfunctions or deviations promptly.

Attachments:

[Include any relevant attachments or reference to attachments]