BIO122-Lab Manual Handout BIO122/OCT 2022-FEB

2023

DATA SHEET FOR EXPERIMENT 1: MICROSCOPY AND THE CELLS

Name: Carin Audryna David

Student Id: 2022897434
Class: AS120F Group: F

Introduction

In compound light microscopes, the word "compound" refers to the microscope's multiple lenses. A
compound microscope, designed with a system of multiple lenses, has two optical components: the objective
lens and the ocular lens. When using a compound light microscope to view an image, you can calculate the
total magnification by multiplying the eyepiece's normal 10x magnification by the objective lens's 4x, 10x, or
40x power. Consequently, a 10x eyepiece combined with a 40X objective lens will result in a 400X
magnification. Microscopic characteristics are made visible since the specimen can now be seen with the
naked eye at a 400-fold higher magnification. The employment of a microscope in academic experiments is
beneficial for students in schools and universities. Using a compound microscope, plant cells are inspected to
identify the microorganisms living within them. Consequently, a compound microscope has demonstrated to
be essential for biologists. (Aryal, 2022)

The stereo microscope, commonly referred to as the dissecting microscope, is the second instrument
utilised in this experiment. This particular form of digital optical microscope uses light that is reflected off
the specimen's surface rather than light that is reflected by the specimen, and thus has a lower magnification
power (5x–250x). Its main function is to dissect samples, view them, and do qualitative analyses on them.
The two different light routes that the microscope's objectives and eyepiece employ determine how the
dissecting microscope operates. Different viewing perspectives are offered by each light path. Both the top
light, used for dissection, and the bottom light, used for viewing the photographs, are present. This lighting is
made possible by the design of two eyepieces (binocular stereoscope), each of which displays a distinct kind
of light pathway and offers a comfortable viewing region. (Mokobi, 2022)

The level of magnification that can be achieved with each of these tools varies greatly, despite the fact
that both are used to enlarge objects that are placed on the microscope's stage. Additionally, they are built
differently, primarily in terms of the objective lenses and the eye parts. The magnification that can be
obtained with each of these devices is one of the key distinctions between a compound and dissecting
microscope. Compound microscopes can often magnify an item by 400 times, although it is not unusual to
discover one that can do so by a factor of 1,000. On the other hand, dissecting microscopes typically only
magnify an object by 40 times or less. (Fox, 2022)
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Result

1)

Compound Light Microscope (Find out the photo and label each part of the
microscope)

Eyepiece

Arm

Objective Lens

Coarse Adjustment
Stage

Fine Adjustment Knob

Light Source

Dissecting Microscope (Find out the photo and label each part of the microscope)

Eyepiece
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Focus Knob

Objective Lens

Switch
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2) Complete the following the table:

Magnification Magnification Total Diameter of Field

of of Magnification
Objectives Eyepiece of Cell’s Image

4 10 40x 4.0mm
X X

10X 10 100x 2.0mm

X

40X 10 400x 0.4mm

X

100X 10 1000x 0.2mm

X

3)

Bacterial Cell
(Attach your drawing/Find out the microscopic photo and provide all the
labels)

Lactobacillus
BIO122-Lab Manual Handout BIO122/OCT 2022-FEB
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Lactobacillus (Magnification, 40x) Streptococcus (Magnification, 40X)

BIO122-Lab Manual Handout BIO122/OCT 2022-FEB
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4)

Animal Cell
(Attach your drawing and provide all the labels)

Cytoplasm

Cell
Membrane

Nucleus

Cheek Cell (Magnification, 40x)

Plant Cell
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2023
(Attach your drawing and provide all the labels)

Cell Wall

Cytoplasm

Onion Cell (Magnification, 10x)