PHCHEM3: Pharmaceutical Analysis 2 (Instrumental Methods of Analysis)

I. Morse Type. Choose the letter of the correct answer from the choices below (2 points x 25 items, total of 50 points)

A. I only B. II only C. III only D. I & II

E. I & III F. II & III G. I, II & III H. Not in the given choices

1. Which of the following is/are CORRECTLY paired?

I. Vitamin A : HPLC
II. Barbiturates : Turbidimetry
III. Vitamin K : Spectrophotometry
None of the above
2. The following is/are correctly paired, EXCEPT:
I. Insulin injection : HPLC
II. Tubocurarine : HPLC
III. Cod liver oil : Turbidimetry
None of the above
3. Which of the following is/are CORRECTLY paired?
I. Cat - Glucagon injection
II. Chicken - Oxytocin injection
III. Dog - Parathyroid injection
None of the above
4. The following is/are correctly paired, EXCEPT:
I. Rabbit - Insulin injection
II. Rat - Heparin
III. Sheep - Vasopressin
None of the above
5. Which of the following is/are TRUE regarding Spectrophotometry?
I. It is a method of analysis which deal with the measurement of spectra
II. It is also a measurement of wavelength in the electromagnetic spectrum
III. It is the measurement of the interactions between light and matter, and the reactions and measurements of radiation intensity and wavelength
None of the above
6. The following pairs of region and wavelength are correct, EXCEPT:
I. Near IR: 3000 – 15000 nm
II. Visible: 380-780 nm
III. UV: 200-380 nm
None of the above
7. Which of the following is/are CORRECT regarding absorbance?
I. Can be computed by getting the product of molar absorptivity, thickness, and concentration.
II. Can be computed by getting the quotient of absorptivity over the product of thickness and concentration
III. It is the quantity of light that passes through a solution.
None of the above
8. The following is/are CORRECT, EXCEPT:
I. A micrometer (um) which is equal to 10^(-4 cm)
II. An Angstrom (Å) which is equal to 10^(-7cm)
III. A nanometer (nm) which is equal to 10^(-8 cm)
None of the above
9. Which of the following is/are TRUE regarding frequency?
I. It is the number of complete cycles which pass a given point per second (cps).
II. It may also be expressed in Hertz.
III. 1 Hertz = 1 cps.
None of the above
10. The following is/are CORRECTLY paired (Wavelength of Color Absorbed in nm - Color absorbed - Complementary color, EXCEPT:
I. 380-450 Violet Yellow
II. 490-500 Blue-Green Red
III. 590-620 Orange Green-blue
None of the above
11. Which of the following are considered as applications of spectrophotometry to pharmaceutical analysis?
I. It determines pKa values, partition coefficients and solubilities of drugs
II. It is a part of dissolution testing
III. It is used in monitoring the pharmacodynamics of drug degradation.
None of the above
12. The following are techniques employed in sample preparation in IR spectroscopy, EXCEPT:
I. Hubl’s technique
II. Mull’s technique
III. Pellet technique
None of the above
13. Which of the following is/are TRUE regarding chromatography?
I. It is a separation technique or process.
II. The components of a mixture are repetitively equilibrated in three phases.
III. It has a mobile phase and a stationary phase.
None of the above
14. The following is/are TRUE, EXCEPT:
I. Stationary phase is also called as eluent or carrier.
II. A stationary phase can be a porous solid used alone or coated with a liquid.
III. The mobile phase can be a liquid or gas that carries through the stationary phase.
None of the above.
15. Which of the following can be assayed by HPLC?
I. Vitamin D
II. Insulin
III. Barbiturates
None of the above
16. The following can be assayed by Gas chromatography, EXCEPT:
I. vitamin E
II. vitamin B6
III. tubocurarine injection
none of the above
17. Which of the following is/are considered as adsorbents?
I. Silica gel
II. Activated alumina
III. Column carbonate
None of the above
18. The following statements/ is/are TRUE regarding HPLC, EXCEPT:
I. It is the most widely used method in drug analysis.
II. It has high resolution column.
III. It uses a low pressure.
None of the above
19. Which of the following are adsorbents used in Thin Layer Chromatography?
I. calcium carbonate
II. alctivated alumina
III. silica gel
None of the above
20. The following is/are HPLC separation modes
I. Reverse phase
II. Ion-exchange
III. Size exclusion
None of the above
21. Bacteria used as biological indicators:
I. B. subtilis
II. B. stearothermiphilus
III. B. pumilus
None of the above
22. Which of the following is/are true regarding picking the number of items per batch in the sterility test?
I. <100 Containers per batch : 10% or 4 containers, whichever is higher
II. 100-500 Containers per batch : 2 % or 20 containers, whichever is less
III. LVP : 2% or 20 containers, whichever is less
None of the above
23. Requirements for Rabbit Pyrogen Test, except:
I. New Zealand or Belgian White
II. Temperature of 39.8 degrees Celsius
III. Female rabbits only
None of the above
24.Procedure in Old Method Pyrogen test
I. Dose: 1 ml / kg body weight
II. Solvent: Sterile water for injection
III. Warm the subjects to 37.0+0.20C prior to injection
None of the above
25. Clarity Test:
I. Coulter Counter Method
II. Slow counting
III. Microscopic identification of particle types and materials
None of the above

II. Modified TRUE or FALSE. Evaluate the following statements whether they are RIGHT or WRONG. Then choose the letter of the CORRECT answer.
(2 points each x 20 items = 40 points)

26. Pyrogen test is used by manufacturers to determine if bacterial toxins are present in vaccines and drugs that might casue fever when used on humans. Solvents could
be a source of pyrogen.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
27. Three healthy rabbits will be observed for rise of temperature at 2-hour interval after injection of sample. Product passed if no rabbit showed an individual rise of
more than 0.50
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
28. Limulus Amoebocyte Lysate Test is an alternative to the Old Method for Pyrogenicity. The amoebocyte is isolated from the urine of horseshoe crab.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
29. Bouger’s Law states that transmittance decrease exponentially, or the thickness increases arithmetically. Beer’s law states that transmittance decrease exponentially,
or the concentration increases arithmetically.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
30. Leaker’s test uses Phenolphthalein Blue. Ampoules are immersed in the solution using a vacuum chamber under negative pressure.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
31. Nephelometry measures reflected light. While turbidimetry measures excess energy lost by emission.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
32. Dispersive IR makes use of a dichromator to select two wavenumbers in turn in order to monitor its intensity after the radiation has passed through the sample.
Fourier transform makes use of interferometer that generates a radiation source in which individual wave numbers can be monitored.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
33. Far IR is from 500 to1000 micrometer. Near IR is 2.5 to 50 micrometer.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
34. Film technique happens when the solid is suspended in a potassium bromide pellet and prepare under a low pressure. Solution technique happens when the sample is
dissolved in a suitable solvent and used as a liquid sample.
A. ONLY statement 1 is TRUE
B.ONLY statement 2 is TRUE
C.BOTH STATEMENTS are TRUE
D.NEITHER of the STATEMENTS are TRUE

35. Gel Clot technique makes use of Limulus polyfamous. Photometric technique makes use of Spectrophotometry concepts.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
36. Stationary phase is a porous solid used alone or coated with liquid. Mobile phase is forced through the stationary phase.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
37. Molecular emission spectroscopy are used in the analysis of vitamin B1 and Vitamin B2. Vitamin B1 is also known as riboflavin, and vitamin B2 is known as
thiamine.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
38. Gas chromatography is used in the analysis of volatile substances and liquids with low boiling point. Paper chromatography is used in the analysis of antigens or
antibodies.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
39. Liquid chromatography that uses high efficiency columns and relatively high mobile phase inlet pressure is called HPLC. HPLC means High-Pressure Liquid
Chromatography
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
40. In column chromatography, the stationary phase are solid adsorbents packed in horizontal glass column. It also used a polar liquid mobile phase added at the top.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
41. Partition is a bulk-phase distribution process in which the solute forms homogenous solutions in each phase. Exclusion involves interaction at the surface or fixed
sites.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
42. Sample is fed onto the column continuously and acts as the mobile phase in frontal sample development. While displacement sample development is characterized
by a mobile phase that is highly attracted to the stationary phase resulting to sample components being pushed through the column by the advancing solvent.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
 D. NEITHER of the STATEMENTS are TRUE

43. Retention time is the time after the sample injection for the small peak of the species is retained by the stationary phase. Retention volume is the volume of carrier
gas that carries average molecule of component from injection point to detector.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
44. Reverse phase happens when the stationary phase is polar, mobile phase is non-polar. The normal phase happens when the stationary phase is non-polar, mobile
phase is polar.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE
45. Gradient elution uses varying composition mobile phase. Isocratic elution uses 1 component mobile phase.
A. ONLY statement 1 is TRUE
B. ONLY statement 2 is TRUE
C. BOTH STATEMENTS are TRUE
D. NEITHER of the STATEMENTS are TRUE

III. Multiple choice. Choose the letter of the CORRECT answer. (1 point each x 20 items = 20 points)
1. Trace minerals in a multivitamin preparation can be assayed faster by:
a. Atomic Absorption Spectrophotometer
b. UV-Vis Spectrophotometer
c. Gas Liquid Chromatography
d. HPLC
2. The instrument used in measuring the optical activity of a sample.
a. Polarimeter
b. Refractometer
c. Spectrometer
d. Flame photometer
3. In IR spectrometry, the fingerprint region is:
a. Near IR
b. Medium IR
c. Far IR
d. Narrow IR
4. All of the following are optical devices or instruments, EXCEPT:
a. pH meter
b. Spectrophotometer
c. Colorimeter
d. Flame photometer
5. Radiant energy required in the analysis of drugs under Fluorometry is in:
a. UV region
b. Visible region
c. IR region
d. Fluorochrome region
6. Nuclear Magnetic Resonance (NMR) is used for:
a. Radioisotopes
b. Irradiation
c. Identification of chemicals
d. A and B
7. One of the fundamental laws in spectrophotometry is:
a. Law of Mass Action
b. Beer’s Law
c. Boyle’s Law
d. Newton’s Law
8. Potentiometry finds application in:
a. Biologic assay
b. pH determination
c. Qualitative analysis
d. A and B
9. A similar preparation in spectrometry refers to:
a. reference standard and sample identically made
b. reference standard greater than sample
c. sample solution greater than reference standard
d. All of the above
10. An instrument in spectrometry using the filter as radiant energy device is:
a. filter photometer
b. infrared spectrometer
c. UV-Vis spectrometer
d. flame photometer
11. Analysis of substances that fluoresce falls under:
a. fluorometry
b. UV spectrometry
c. turbidimetry
d. A and B
12. What is being read in spectrophotometer:
a. concentration error
 b. absorbance
c. retention
d. angular rotation
13. The type of chromatography where the cellulose of the filter paper is sued as the adsorbent.
a. Column
b. Gas
c. Paper
d. TLC
14. The most widely used apparatus being used for the determination of drug mixtures and components.
a. NMR
b. HPLC
c. GC
d. NMR
15. Gas chromatography is utilized in the separation of:
a. volatile liquids
b. amino acids
c. carbohydrates
d. lipids
16. In Thin layer chromatography, separation takes place on a planar surface by:
a. differential migration
b. electrostatic force
c. relative solubility
d. capillary action
17. The separation of the components of an organic dye mixture is accomplished using:
a. Paper chromatography
b. Thin layer chromatography
c. Partition chromatography
d. Gas chromatography
18. The following are the basic principles upon which chromatographic separations depend upon, EXCEPT:
a. Partition
b. Absorption
c. Adsorption
d. Ion-exchange
19. The heart of HPLC and GC:
a. Pumps
b. Injection valve
c. Column
d. Detectors
20. Gas chromatography uses a stationary phase as a:
a. gas
b. liquid
c. substrate
d. cellulose