1) This document provides a review of key concepts in general biology for a 2nd monthly examination, including macromolecules like carbohydrates, lipids, nucleic acids, and proteins.
2) It defines enzymes as catalytic proteins that speed up reactions by lowering activation energy barriers through substrate binding and an induced fit mechanism in their active sites.
3) The review covers factors that affect enzyme function like temperature and pH, as well as types of enzyme inhibitors like competitive and noncompetitive inhibitors.
1) This document provides a review of key concepts in general biology for a 2nd monthly examination, including macromolecules like carbohydrates, lipids, nucleic acids, and proteins.
2) It defines enzymes as catalytic proteins that speed up reactions by lowering activation energy barriers through substrate binding and an induced fit mechanism in their active sites.
3) The review covers factors that affect enzyme function like temperature and pH, as well as types of enzyme inhibitors like competitive and noncompetitive inhibitors.
1) This document provides a review of key concepts in general biology for a 2nd monthly examination, including macromolecules like carbohydrates, lipids, nucleic acids, and proteins.
2) It defines enzymes as catalytic proteins that speed up reactions by lowering activation energy barriers through substrate binding and an induced fit mechanism in their active sites.
3) The review covers factors that affect enzyme function like temperature and pH, as well as types of enzyme inhibitors like competitive and noncompetitive inhibitors.
Carbon Compounds at least one carbon-carbon double Macromolecules – giant molecules bond c) Polyunsaturated Fatty Acids – - Made up of thousands or hundreds contain more than one double bond of thousands of smaller molecules of carbon-carbon - Polymerization: a process in which 4) Nucleic Acids – contains carbon, large compounds are built by joining hydrogen, oxygen, nitrogen, phosphorus smaller ones together (monomers) (CHONP) to form polymers - Nucleotides: polymers assembled 1) Carbohydrates – made of carbon, from individual monomers hydrogen, and oxygen in a ratio of 1:2:1 o Consists of three parts - Used by living organisms as their ▪ 5-carbon sugar main source of energy ▪ Phosphate group - Also used by some animals or plants ▪ Nitrogenous base for structural purposes - Stores and transmits hereditary or - Starches: extra sugar that is stored genetic information (complex carbohydrates) - RNA (Ribonucleic Acid) and DNA a) Monosaccharide – single sugar (Deoxyribonucleic Acid) a. Galactose – milk sugar 5) Proteins – contains carbon, hydrogen, b. Fructose – fruit sugar oxygen, and nitrogen (CHON) c. Glucose – plant sugar - Amino Acids: polymers of b) Disaccharide – two molecules. Compounds with an monosaccharides amino group (-NH2) and a carboxyl a. Sucrose (table sugar) – group (-COOH) glucose + fructose - R-Group: side chain of amino acid b. Lactose – glucose + o Can be acidic or basic galactose o Polar or nonpolar c) Polysaccharide – large molecules o Can contain carbon rings formed from monosaccharides - DNA is responsible in giving - Glycogen (Animal starch): a instructions on arranging amino polysaccharide where animals store acids excess sugar - Can control the rate of reactions and - Cellulose: plant starch that gives regulate cell processes plants’ strength and rigidity 2) Lipids – aren’t soluble in water and Enzymes made of mostly carbon and hydrogen Catalysts – chemical agent that speeds up (sometimes there is oxygen) a reaction without being consumed by the 3) reaction - Fats, oils, and waxes are common categories of lipids Enzyme – a catalytic protein - Can be used to store energy - (-ase) to determine an enzyme - They are formed when glycerol - Enzymes speed up the reaction by combines with fatty acids lowering energy barriers a) Saturated Fatty Acids – maximum possible number of hydrogen atoms - Single bonded carbon GENERAL BIOLOGY Ü 2ND MONTHLY EXAMINATIONS REVIEWER Goodluck!
o Orienting substrates correctly
o Staining substrate bonds o Providing a favorable microenvironment o Covalently bonding to the The Activation Energy Barrier – initial substrate energy needed to start a chemical reaction - Supplied in the form of thermal energy that the reactant molecules absorb from their surroundings
Factors that Affect Enzyme Function
1) Effects of Temperature and pH – each enzyme has an optimal temperature and pH level in which it can function
How Enzymes Lower the EA Barrier
- Enzymes catalyze reactions by lowering the EA barrier - They do not affect the change in free energy, they hasten reactions Substrate Specificity of Enzymes - Substrate – reactant that an enzyme act on - Enzyme-subtrate complex – enzyme binds to substrate - Active site – region on the enzyme where the substrate binds - Induced fit – enzyme will always cater a substrate Catalysis in the Enzyme’s Active Site Metabolism = Catabolism + Anabolism - Substrate binds to the active site of the enzyme a) Catabolism – breaking down of - The active site can lower an EA substances; produce energy barrier by: GENERAL BIOLOGY Ü 2ND MONTHLY EXAMINATIONS REVIEWER Goodluck!
b) Anabolism – form substances;
requires energy Cofactors - nonprotein enzyme helpers - cofactors may be inorganic or organic - Coenzyme – organic cofactor Enzyme Inhibitors a) Competitive Inhibitors – bind to the active site of an enzyme, competing with a substrate b) Noncompetitive Inhibitors – binds to another part of an enzyme causing the enzyme to change shape and making the active site less effective - Examples of Inhibitors: toxins, poisons, pesicides, antibiotics
Amino Acids are linked by peptide bonds to form formed by linking the α-carboxyl group of one amino acid to the α-amino group of another amino acid with a peptide bond (also called an amide bond)