Dakota Peat and Equipment

833 Gateway drive NE, Minnesota

East Grand Forks, Minnesota, 56721, United States

Microbiome Analysis Report

DAKOTA_PEAT DI9000
DATE
9-Jan-2024

Microbial Population
All the information shown in this microbial report is based on the detected presence of 811 different species.
FU NG AL B AC T ER I AL

P H YL U M D I S T R I B U T I O N P H YL U M D I S T R I B U T I O N

Basidiomycota 75% Actinobacteriota 37%

Ascomycota 11% Proteobacteria 32%

Mortierellomycota 4% Fungus Bacteria Bacteroidota 11%

Mucoromycota < 1% Verrucomicrobiota 6%

Acidobacteriota 5%

Conclusions
STRENGTHS

Carbon xation 69%

Inorganic nitrogen release 45%

Biosustainability

BIODIVERSITY FUNCTIONALITY

5.76 3.44
0 Richness, evenness and 10 0 Capability of soil microbial 10
distinctness of microbial species communities to perform
multiple functions

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Plant health improvement

Biocontrol agents, plant growth promoting organisms

BIOCONTROL
Microbial species grouped according to the type of pest they encounter, capable of preventing pathogenic species from taking hold or
proliferation
Fungicide agents Bactericide agents
6% NOT DETECTED

Insecticide agents Nematicide agents

NOT DETECTED 6%

HORMONE PRODUCTION
Microbial species grouped according to the type of phytohormone they generate
Auxin production (IAA) Cytokinin production (CK)
CELL DIVISION STEM ELONGATION CELL PROLIFERATION CELL DIFFERENTIATION

42% 12%

Gibberellin production (GA)

STEM ELONGATION GERMINATION FLOWERING

8%

STRESS ADAPTATION
Microbial species grouped according to their relationship with the metabolisms linked to the capability to withstand stress conditions
Exopolysaccharide production ACC deaminase (ACC-d)
NUTRIENT TRAP SALINITY PROTECT. DROUGHT PROTECT. PATHOGEN PROTECT. SALINITY PROTECT. DROUGHT PROTECT.

16% 46%

Heavy metal resistance Salicylic acid (SA)

BIOREMEDIATION DETOXIFICATION ALLEVIATE HEAVY METAL STRESS DROUGHT PROTECT. SALINITY PROTECT. ALLEVIATE HEAVY METAL STRESS

14% 14%

Salt tolerance Abscisic acid (ABA)

SALINITY PROTECT. ROOT GROWTH PROMOTION GROWTH REGULATION PLANT RESISTANCE INCREASE YIELDS

13% 6%

Siderophore production
IRON AVAILABILITY BIOFERTILIZER

11%

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Nutrition
Nutritional status based on the microbial mobilization of certain compounds

M A J O R C O M P O U N D S

C Carbon N Nitrogen

GAIN NUTRIENT SUPPLY

Carbon xation 69% Inorganic nitrogen release 45%

LOSS NUTRIENT COMPETITION

Aerobic respiration 58% Inorganic nitrogen consumption 73%
Fermentation 15%
Methanogenesis 40% INDIRECT BENEFITS
Nitrogen cycle 17%
INDIRECT BENEFITS
Organic matter release 68%

P Phosphorus K Potassium

NUTRIENT SUPPLY NUTRIENT SUPPLY

Inorganic P solubilization 28% Potassium solubilization 28%

NUTRIENT COMPETITION NUTRIENT COMPETITION

Inorganic P consumption 20% Potassium consumption 55%

INDIRECT BENEFITS
Organic P assimilation 61%

M I N O R C O M P O U N D S

Iron Calcium
Fe Iron assimilation 58% Ca Calcium transport 44%

Zinc Copper
Zn Zinc transport equilibrium 60% Cu Copper export 48%

Manganese
Magnesium
Mn Manganese transport
equilibrium
25% Mg Magnesium transport 41%

Sulfur Chlorine
S Sulfur cycle equilibrium 20% Cl Chlorine transport 53%

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APPENDIX
Bacterial Quantification 2.0
Methodology
The Next-Gen sequencing in combination with the addition of a known quantity spike-in enables the knowledge of the total microbial load in a sample.
The present analysis relies on the application of a spike-in of our synthetic proprietary DNA sequence in known quantities into crude samples. After the
sequencing and data processing, the relative abundance of the exogenous spike-in allows us to extrapolate the original absolute quantity of the 16S
copies of the sample species, while knowledge of the number of gene copies per genome in the species allows us to calculate the number of Cells.
Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

1 Udaeobacter sp. 1.02e+7 16 Pedomicrobium sp. 1.45e+6

2 Pseudolabrys sp. 6.69e+6 17 Kribbella endophytica 1.28e+6

3 Nocardioides sp. 5.34e+6 18 Ignavibacterium sp. 1.28e+6

4 Saccharopolyspora sp. 3.06e+6 19 Gemmatimonas sp. 1.21e+6

5 Nitrosotenuis sp. 2.69e+6 20 Pirellula sp. 1.16e+6

6 Acidibacter sp. 2.62e+6 21 Nitrospira sp. 1.08e+6

7 Rhodoplanes sp. 2.18e+6 22 Nitrosocosmicus oleophilus 1.02e+6

8 Hyphomicrobium sp. 2.01e+6 23 Brevundimonas sp. 1.01e+6

9 Bauldia sp. 2.00e+6 24 Sphingomonas sp. 1.00e+6

10 Bryobacter sp. 1.98e+6 25 Streptomyces sp. 9.97e+5

11 Gaiella sp. 1.89e+6 26 Thermomonas brevis 9.97e+5

12 Stenotrophobacter sp. 1.63e+6 27 Acidothermus sp. 9.42e+5

13 Altererythrobacter sp. 1.60e+6 28 Marmoricola sp. 9.16e+5

14 Solibacter sp. 1.56e+6 29 Reyranella sp. 9.09e+5

15 Terrimonas sp. 1.52e+6 30 Conexibacter sp. 9.02e+5

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Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

31 Kribbella sp. 8.78e+5 53 Phenylobacterium sp. 5.32e+5

32 Actinomadura sp. 8.71e+5 54 Haliangium sp. 5.23e+5

33 Pedococcus aerophilus 8.53e+5 55 Segetibacter sp. 5.16e+5

34 Streptomyces chumphonensis 8.39e+5 56 Pseudarthrobacter oxydans 5.02e+5

35 Nocardioides kribbensis 8.19e+5 57 Ramlibacter sp. 5.00e+5

36 Chthoniobacter sp. 8.16e+5 58 Pedobacter insulae 4.93e+5

37 Iamia sp. 7.78e+5 59 Sideroxydans sp. 4.92e+5

38 Dyella ginsengisoli 7.59e+5 60 Edaphobaculum sp. 4.87e+5

39 Massilia sp. 7.52e+5 61 Ferruginibacter sp. 4.38e+5

40 Lacunisphaera sp. 7.34e+5 62 Koribacter sp. 4.32e+5

41 Opitutus sp. 7.30e+5 63 Sphingosinicella vermicomposti 4.30e+5

42 Bradyrhizobium sp. 6.82e+5 64 Flavobacterium sp. 4.11e+5

43 Pedobacter sp. 6.77e+5 65 Dongia sp. 3.83e+5

44 Bdellovibrio sp. 6.74e+5 66 Arenimonas sp. 3.76e+5

45 Lysobacter pocheonensis 6.58e+5 67 Streptomyces sulfonofaciens 3.53e+5

46 Lysobacter sp. 6.45e+5 68 Saccharopolyspora gregorii 3.36e+5

47 Actinophytocola sp. 5.92e+5 69 Streptomyces echinoruber 3.25e+5

48 Leptolinea sp. 5.82e+5 70 Devosia insulae 3.15e+5

49 Solirubrobacter sp. 5.78e+5 71 Spirochaeta sp. 3.05e+5

50 Streptomyces thermocarboxydus 5.57e+5 72 Methanoregula sp. 2.90e+5

51 Aquicella sp. 5.44e+5 73 Janibacter melonis 2.75e+5

52 Lysobacter ginsengisoli 5.38e+5 74 Hirschia sp. 2.73e+5

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Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

75 Luteitalea sp. 2.69e+5 97 Solirubrobacter soli 1.91e+5

76 Thermoanaerobaculum sp. 2.67e+5 98 Mucilaginibacter sp. 1.78e+5

77 Nocardioides alpinus 2.56e+5 99 Nordella sp. 1.76e+5

78 Actinopolymorpha sp. 2.56e+5 100 Gaiella occulta 1.73e+5

79 Brevundimonas subvibrioides 2.53e+5 101 Kitasatospora mediocidica 1.70e+5

80 Nitrosocosmicus sp. 2.52e+5 102 Marmoricola ginsengisoli 1.65e+5

81 Caulobacter henricii 2.50e+5 103 Streptomyces avermitilis 1.65e+5

82 Lysobacter dokdonensis 2.46e+5 104 Nocardioides koreensis 1.62e+5

83 Chryseobacterium jeonii 2.44e+5 105 Phenylobacterium koreense 1.56e+5

84 Mycobacterium madagascariense 2.26e+5 106 Variovorax paradoxus 1.54e+5

85 Devosia sp. 2.24e+5 107 Nitrososphaera viennensis 1.52e+5

86 Mycobacterium sp. 2.20e+5 108 Actinoallomurus spadix 1.51e+5

87 Nitrososphaera sp. 2.18e+5 109 Nakamurella endophytica 1.51e+5

88 Phenylobacterium mobile 2.16e+5 110 Hydrogenophaga palleronii 1.49e+5

89 Thermomonas sp. 2.14e+5 111 Ilumatobacter sp. 1.48e+5

90 Aridibacter sp. 2.04e+5 112 Glycomyces sp. 1.47e+5

91 Rhizobacter sp. 2.01e+5 113 Alterococcus sp. 1.46e+5

92 Massilia timonae 1.99e+5 114 Sphingomonas echinoides 1.44e+5

93 Mesorhizobium sp. 1.98e+5 115 Lysobacter soli 1.44e+5

94 Pajaroellobacter sp. 1.96e+5 116 Pseudorhodoplanes sp. 1.42e+5

95 Occallatibacter sp. 1.95e+5 117 Blastocatella sp. 1.40e+5

96 Fictibacillus nanhaiensis 1.91e+5 118 Methanobacterium sp. 1.39e+5

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Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

119 Aeromicrobium ginsengisoli 1.34e+5 141 Caulobacter sp. 9.35e+4

120 Povalibacter uvarum 1.34e+5 142 Brevitalea deliciosa 9.25e+4

121 Flavisolibacter sp. 1.25e+5 143 Hephaestia sp. 9.25e+4

122 Coxiella sp. 1.23e+5 144 Nocardioides pyridinolyticus 9.05e+4

123 Luteimonas sp. 1.21e+5 145 Herminiimonas fonticola 9.05e+4

124 Gemmata sp. 1.20e+5 146 Stenotrophomonas rhizophila 8.22e+4

125 Sphingomonas daechungensis 1.19e+5 147 Methanosaeta sp. 8.12e+4

126 Altererythrobacter dongtanensis 1.19e+5 148 Methylibium sp. 8.02e+4

127 Leptothrix sp. 1.18e+5 149 Methylobacter tundripaludum 8.02e+4

128 Sphingomonas soli 1.17e+5 150 Omnitrophus sp. 8.02e+4

129 Acidovorax sp. 1.16e+5 151 Fluviicola sp. 7.71e+4

130 Woykebacteria sp. 1.13e+5 152 Lacunisphaera limnophila 7.61e+4

131 Rhodanobacter sp. 1.11e+5 153 Peredibacter starrii 7.61e+4

132 Mucilaginibacter calamicampi 1.10e+5 154 Methanocella sp. 7.50e+4

133 Chitinophaga sp. 1.07e+5 155 Acidicaldus sp. 7.45e+4

134 Methyloceanibacter sp. 1.07e+5 156 Methanomassiliicoccus sp. 7.40e+4

135 Stenotrophobacter terrae 1.07e+5 157 Caulobacter vibrioides 7.40e+4

136 Nitrosospira lacus 1.05e+5 158 Sphaerobacter sp. 7.40e+4

137 Anaerolinea sp. 9.97e+4 159 Aeromicrobium sp. 7.40e+4

138 Geobacter sp. 9.76e+4 160 Streptomyces atratus 7.37e+4

139 Pedosphaera sp. 9.76e+4 161 Ideonella sp. 7.26e+4

140 Streptomyces abyssalis 9.39e+4 162 Mucilaginibacter kameinonensis 7.19e+4

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Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

163 Terrimicrobium sp. 7.19e+4 185 Mucilaginibacter lutimaris 5.62e+4

164 Dokdonella sp. 7.09e+4 186 Actinomadura rubrobrunea 5.59e+4

165 Sphingopyxis alaskensis 6.78e+4 187 Desulfobacca sp. 5.55e+4

166 Jatrophihabitans sp. 6.78e+4 188 Methylophilus sp. 5.45e+4

167 Pseudonocardia zijingensis 6.72e+4 189 Actinoallomurus sp. 5.43e+4

168 Adhaeribacter sp. 6.63e+4 190 Phaselicystis sp. 5.41e+4

169 Syntrophus sp. 6.58e+4 191 Sphingopyxis taejonensis 5.34e+4

170 Pseudonocardia acaciae 6.51e+4 192 Bosea sp. 5.34e+4

171 Asticcacaulis sp. 6.44e+4 193 Fimbriiglobus sp. 5.24e+4

172 Novosphingobium sp. 6.37e+4 194 Rhizomicrobium electricum 5.24e+4

173 Oryzihumus sp. 6.37e+4 195 Alkanibacter sp. 5.24e+4

174 Shimazuella sp. 6.30e+4 196 Porphyrobacter tepidarius 5.24e+4

175 Mucilaginibacter rigui 6.24e+4 197 Aquisphaera sp. 5.21e+4

176 Para limonas sp. 6.17e+4 198 Emticicia aquatica 5.21e+4

177 Roseimicrobium sp. 6.10e+4 199 Spirosoma sp. 5.21e+4

178 Pseudomonas mandelii 6.03e+4 200 Paludibaculum sp. 5.04e+4

179 Woesebacteria sp. 5.96e+4 201 Rhizobium sp. 5.00e+4

180 Sphingomonas mucosissima 5.76e+4 202 Sphingomonas ava 4.93e+4

181 Taibaiella sp. 5.69e+4 203 Methanosarcina sp. 4.87e+4

182 Sphingomonas jaspsi 5.65e+4 204 Peredibacter sp. 4.83e+4

183 Singulisphaera sp. 5.63e+4 205 Microlunatus ginsengiterrae 4.73e+4

184 Prosthecobacter sp. 5.62e+4 206 Thermasporomyces composti 4.73e+4

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Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

207 Sphingomonas sediminicola 4.73e+4 229 Steroidobacter sp. 3.91e+4

208 Legionella sp. 4.59e+4 230 Para limonas rhizosphaerae 3.91e+4

209 Xiphinematobacter sp. 4.52e+4 231 Sphingobium sp. 3.91e+4

210 Micromonospora sp. 4.52e+4 232 Amycolatopsis taiwanensis 3.80e+4

211 Niastella sp. 4.52e+4 233 Sumerlaea sp. 3.80e+4

212 Blastococcus sp. 4.52e+4 234 Nannocystis sp. 3.80e+4

213 Bordetella bronchialis 4.45e+4 235 Sphingomonas psychrolutea 3.80e+4

214 Sandaracinus sp. 4.42e+4 236 Arcticibacter sp. 3.80e+4

215 Haliscomenobacter sp. 4.42e+4 237 Arenimonas metalli 3.70e+4

216 Nocardia paucivorans 4.39e+4 238 Chryseolinea sp. 3.70e+4

217 Pedobacter namyangjuensis 4.37e+4 239 Nocardioides pelophilus 3.60e+4

218 Planctopirus sp. 4.32e+4 240 Aggregicoccus edonensis 3.56e+4

219 Puia sp. 4.25e+4 241 Solibacter usitatus 3.49e+4

220 Rhodopseudomonas sp. 4.21e+4 242 Luteibacter sp. 3.49e+4

221 Phreatobacter sp. 4.21e+4 243 Ohtaekwangia sp. 3.43e+4

222 Asprobacter aquaticus 4.21e+4 244 Methylobacillus sp. 3.29e+4

223 Sphingomonas faeni 4.21e+4 245 Flavobacterium aquidurense 3.17e+4

224 Flavisolibacter metallilatus 4.21e+4 246 Nitrotoga sp. 3.15e+4

225 Para limonas terrae 4.18e+4 247 Amycolatopsis nigrescens 3.13e+4

226 Nitrosoarchaeum limnia 4.11e+4 248 Brevundimonas basaltis 3.08e+4

227 Vicinamibacter sp. 3.91e+4 249 Flaviaesturariibacter sp. 3.08e+4

228 Bradyrhizobium cytisi 3.91e+4 250 Caulobacter fusiformis 3.08e+4

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Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

251 Herminiimonas glaciei 3.08e+4 273 Pseudomonas brassicacearum 2.75e+4

252 Erythrobacter mathurensis 3.08e+4 274 Flavitalea sp. 2.74e+4

253 Chryseobacterium montanum 3.02e+4 275 Woeseia sp. 2.67e+4

254 Taibaiella smilacinae 3.01e+4 276 Bacteriovorax sp. 2.67e+4

255 Pseudoduganella sp. 3.01e+4 277 Rhizomicrobium sp. 2.67e+4

256 Lentzea kentuckyensis 3.00e+4 278 Abditibacterium sp. 2.67e+4

257 Anaeromyxobacter sp. 2.98e+4 279 Phaeodactylibacter sp. 2.67e+4

258 Massilia haematophila 2.97e+4 280 Angustibacter luteus 2.62e+4

259 Hymenobacter sp. 2.95e+4 281 Persicitalea sp. 2.60e+4

260 Pseudoxanthomonas wuyuanensis 2.88e+4 282 Crenothrix sp. 2.57e+4

261 Filomicrobium sp. 2.88e+4 283 Brevundimonas intermedia 2.57e+4

262 Lapillicoccus sp. 2.88e+4 284 Flindersiella endophytica 2.57e+4

263 Larkinella sp. 2.88e+4 285 Sediminibacterium sp. 2.47e+4

264 Altererythrobacter mangrovi 2.88e+4 286 Nitrosospira multiformis 2.47e+4

265 Chryseobacterium sp. 2.88e+4 287 Cephaloticoccus sp. 2.47e+4

266 Mucilaginibacter carri 2.81e+4 288 Ovatusbacter sp. 2.47e+4

267 Angustibacter sp. 2.78e+4 289 Tardiphaga robiniae 2.47e+4

268 Rhodoferax saidenbachensis 2.78e+4 290 Pedomicrobium manganicum 2.47e+4

269 Sulfuritalea sp. 2.78e+4 291 Moranbacteria sp. 2.47e+4

270 Alsobacter metallidurans 2.78e+4 292 Altererythrobacter buctensis 2.47e+4

271 Niabella sp. 2.78e+4 293 Chthonomonas sp. 2.47e+4

272 Nocardioides furvisabuli 2.78e+4 294 Rhodomicrobium sp. 2.47e+4

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Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

295 Micropepsis sp. 2.36e+4 317 Smithella sp. 2.06e+4

296 Adhaeribacter terrae 2.36e+4 318 Phenylobacterium haematophilum 2.06e+4

297 Lysinimonas soli 2.36e+4 319 Tundrisphaera sp. 2.06e+4

298 Terrabacter sp. 2.26e+4 320 Rhodococcus sp. 2.06e+4

299 Lysobacter concretionis 2.26e+4 321 Aquicella siphonis 2.06e+4

300 Edaphobacter sp. 2.26e+4 322 Hymenobacter gelipurpurascens 1.99e+4

301 Conexibacter woesei 2.26e+4 323 Pseudoxanthomonas gei 1.95e+4

302 Azambacteria sp. 2.26e+4 324 Brevundimonas variabilis 1.95e+4

303 Nitrospira japonica 2.26e+4 325 Methylorosula sp. 1.92e+4

304 Anammoximicrobium sp. 2.26e+4 326 Luteolibacter sp. 1.92e+4

305 Microlunatus soli 2.26e+4 327 Aureimonas glaciei 1.92e+4

306 Desulfatiglans sp. 2.19e+4 328 Glutamicibacter nicotianae 1.85e+4

307 Rhizobium soli 2.19e+4 329 Undibacterium sp. 1.85e+4

308 Dinghuibacter sp. 2.19e+4 330 Variibacter sp. 1.85e+4

309 Nocardioides marinus 2.16e+4 331 Ferruginibacter profundus 1.85e+4

310 Dokdonella ginsengisoli 2.16e+4 332 Achromobacter xylosoxidans 1.85e+4

311 Curvibacter sp. 2.12e+4 333 Marmoricola scoriae 1.85e+4

312 Luedemannella sp. 2.12e+4 334 Sporichthya sp. 1.85e+4

313 Crossiella sp. 2.11e+4 335 Arenimonas oryziterrae 1.75e+4

314 Bradyrhizobium jicamae 2.06e+4 336 Sphingorhabdus rigui 1.75e+4

315 Peregrinibacteria sp. 2.06e+4 337 Syntrophorhabdus sp. 1.75e+4

316 Luteitalea pratensis 2.06e+4 338 Gemmatirosa sp. 1.75e+4

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Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

339 Methylocystis sp. 1.75e+4 361 Nakamurella sp. 1.64e+4

340 Janibacter cremeus 1.75e+4 362 Yinghuangia aomiensis 1.64e+4

341 Microbacterium sp. 1.75e+4 363 Rhodovastum sp. 1.59e+4

342 Methanobacterium ferruginis 1.75e+4 364 Massilia brevitalea 1.59e+4

343 Actinocatenispora sp. 1.71e+4 365 Legionella tucsonensis 1.58e+4

344 Acidovorax facilis 1.71e+4 366 Methylobacterium adhaesivum 1.56e+4

345 Mucilaginibacter boryungensis 1.71e+4 367 Crocinitomix sp. 1.54e+4

346 Pedobacter duraquae 1.70e+4 368 Aureispira sp. 1.54e+4

347 Jahnella thaxteri 1.70e+4 369 Aquihabitans daechungensis 1.54e+4

348 Plani lum sp. 1.68e+4 370 Solimonas sp. 1.54e+4

349 Magasanikbacteria sp. 1.64e+4 371 Segetibacter aerophilus 1.51e+4

350 Erythrobacter sp. 1.64e+4 372 Gallionella sp. 1.51e+4

351 Bythopirellula sp. 1.64e+4 373 Acinetobacter baumannii 1.51e+4

352 Hyphomicrobium nitrativorans 1.64e+4 374 Pseudomonas koreensis 1.48e+4

353 Yanofskybacteria sp. 1.64e+4 375 Tumebacillus ginsengisoli 1.44e+4

354 Berkiella sp. 1.64e+4 376 Silvanigrella sp. 1.44e+4

355 Methylibium petroleiphilum 1.64e+4 377 Thiothrix sp. 1.44e+4

356 Nitrospira nitri cans 1.64e+4 378 Luteibacter rhizovicinus 1.44e+4

357 Thiobacter sp. 1.64e+4 379 Herpetosiphon sp. 1.44e+4

358 Advenella mimigardefordensis 1.64e+4 380 Aeromicrobium panaciterrae 1.44e+4

359 Fluviicola ri ensis 1.64e+4 381 Cellulomonas sp. 1.44e+4

360 Variovorax ginsengisoli 1.64e+4 382 Runella palustris 1.44e+4

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Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

383 Pedobacter daechungensis 1.44e+4 405 Caenimonas sp. 1.30e+4

384 Knoellia ava 1.44e+4 406 Herbaspirillum sp. 1.30e+4

385 Niabella ginsengisoli 1.44e+4 407 Mitsuaria chitosanitabida 1.30e+4

386 Jatrophihabitans endophyticus 1.44e+4 408 Acinetobacter calcoaceticus 1.30e+4

387 Noviherbaspirillum suwonense 1.44e+4 409 Amycolatopsis sp. 1.28e+4

388 Micromonospora chokoriensis 1.44e+4 410 Geobacter bemidjiensis 1.28e+4

389 Terrimonas soli 1.44e+4 411 Nocardioides antarcticus 1.23e+4

390 Pseudonocardia sp. 1.44e+4 412 Homoserinibacter gongjuensis 1.23e+4

391 Sulfurifustis sp. 1.44e+4 413 Brevundimonas vesicularis 1.23e+4

392 Nitrospira de uvii 1.44e+4 414 Abawacabacteria sp. 1.23e+4

393 Nitrosarchaeum sp. 1.44e+4 415 Mycobacterium agri 1.23e+4

394 Actinocorallia sp. 1.40e+4 416 Chitinophaga taiwanensis 1.23e+4

395 Pedobacter metabolipauper 1.39e+4 417 Parasegetibacter sp. 1.23e+4

396 Hassallia sp. 1.37e+4 418 Blastopirellula sp. 1.23e+4

397 Syntrophobacter sp. 1.34e+4 419 Zhizhongheella caldifontis 1.23e+4

398 Sphingorhabdus sp. 1.34e+4 420 Acidovorax dela eldii 1.23e+4

399 Schlesneria sp. 1.34e+4 421 Sphingomonas hengshuiensis 1.23e+4

400 Pseudoxanthomonas humi 1.34e+4 422 Bradyrhizobium canariense 1.23e+4

401 Thermobispora bispora 1.34e+4 423 Nitrosomonas sp. 1.23e+4

402 Polaromonas sp. 1.34e+4 424 Plani lum fulgidum 1.20e+4

403 Actinoallomurus vinaceus 1.32e+4 425 Stenotrophomonas maltophilia 1.18e+4

404 Rurimicrobium sp. 1.30e+4 426 Tumebacillus sp. 1.13e+4

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Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

427 Tenderia sp. 1.13e+4 449 Acuticoccus sp. 1.03e+4

428 Flavisolibacter ginsengisoli 1.13e+4 450 Quadrisphaera sp. 1.03e+4

429 Methylobacter sp. 1.13e+4 451 Protochlamydia sp. 1.03e+4

430 Nitrolancea sp. 1.13e+4 452 Dyadobacter fermentans 1.03e+4

431 Flavobacterium soli 1.12e+4 453 Amycolatopsis mediterranei 9.77e+3

432 Hamadaea sp. 1.10e+4 454 Thermo avi lum sp. 9.59e+3

433 Aurantisolimonas sp. 1.10e+4 455 Duganella zoogloeoides 9.59e+3

434 Rhodobacter megalophilus 1.10e+4 456 Micromonospora echinospora 9.59e+3

435 Actinomycetospora sp. 1.08e+4 457 Spirosoma linguale 9.59e+3

436 Comamonas sp. 1.07e+4 458 Luteibacter yeojuensis 9.25e+3

437 Jidaibacter sp. 1.03e+4 459 Ahniella sp. 9.25e+3

438 Microbacterium pygmaeum 1.03e+4 460 Brevundimonas bullata 9.25e+3

439 Microlunatus aurantiacus 1.03e+4 461 Tenggerimyces sp. 9.25e+3

440 Amphiplicatus sp. 1.03e+4 462 Thermoactinomyces sp. 9.10e+3

441 Nocardioides hungaricus 1.03e+4 463 Actinoplanes friuliensis 8.91e+3

442 Sphingoaurantiacus polygranulatus 1.03e+4 464 Flavihumibacter stibioxidans 8.91e+3

443 Antricoccus sp. 1.03e+4 465 Bordetella petrii 8.91e+3

Microbacterium Paludisphaera
444 arabinogalactanolyticum
1.03e+4 466 sp. 8.91e+3

445 Nocardioides plantarum 1.03e+4 467 Paenarthrobacter sp. 8.57e+3

446 Sphingomonas antarctica 1.03e+4 468 Sporosarcina luteola 8.48e+3

447 Devosia submarina 1.03e+4 469 Variovorax soli 8.22e+3

448 Pedococcus dokdonensis 1.03e+4 470 Brevundimonas kwangchunensis 8.22e+3

#DI9000 14
 Dakota Peat and Equipment

Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

471 Methylocella sp. 8.22e+3 493 Cystobacter gracilis 7.71e+3

472 Rubellimicrobium sp. 8.22e+3 494 Flavobacterium xinjiangense 7.64e+3

473 Nocardioides terrae 8.22e+3 495 Fictibacillus rigui 7.64e+3

474 Roseisolibacter agri 8.22e+3 496 Phyllobacterium myrsinacearum 7.54e+3

475 Hydrogenispora sp. 8.22e+3 497 Nocardia vinacea 7.54e+3

476 Labrys sp. 8.22e+3 498 Plantactinospora mayteni 7.54e+3

477 Pseudohongiella sp. 8.22e+3 499 Lacibacter sp. 7.54e+3

478 Polycyclovorans sp. 8.22e+3 500 Adhaeribacter terreus 7.20e+3

479 Mycetocola sp. 8.22e+3 501 Dyadobacter psychrophilus 7.20e+3

480 Pedobacter steynii 8.22e+3 502 Lihuaxuella sp. 7.19e+3

481 Acidisphaera sp. 8.22e+3 503 Sphingomonas changbaiensis 7.19e+3

482 Erythrobacter atlanticus 8.22e+3 504 Amnibacterium sp. 7.19e+3

483 Vogelbacteria sp. 8.22e+3 505 Frankia sp. 7.19e+3

484 Hyphomicrobium denitri cans 8.22e+3 506 Methylotenera sp. 7.19e+3

485 Methylovirgula ligni 8.22e+3 507 Hansschlegelia plantiphila 7.19e+3

486 Mycobacterium tusciae 8.22e+3 508 A pia sp. 7.19e+3

487 Rhodopirellula sp. 8.22e+3 509 Armatimonas sp. 7.19e+3

488 Tellurimicrobium multivorans 8.22e+3 510 Lewinella sp. 7.19e+3

489 Granulicella sp. 8.22e+3 511 Enhygromyxa sp. 7.19e+3

490 Roseomonas sp. 7.81e+3 512 Stigmatella sp. 7.19e+3

491 Neobacillus drentensis 7.77e+3 513 Emticicia ginsengisoli 6.85e+3

492 Methanospirillum sp. 7.71e+3 514 Cupriavidus campinensis 6.68e+3

#DI9000 15
 Dakota Peat and Equipment

Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

515 Pedobacter lithocola 6.68e+3 537 Cytophaga sp. 5.48e+3

516 Sorangium sp. 6.68e+3 538 Methylobacterium radiotolerans 5.48e+3

517 Actinoallomurus coprocola 6.58e+3 539 Sediminibacterium aquarii 5.48e+3

518 Sphingopyxis indica 6.17e+3 540 Lacihabitans soyangensis 5.48e+3

519 Citrifermentans sp. 6.17e+3 541 Chelativorans multitrophicus 5.48e+3

520 Deinococcus radioresistens 6.17e+3 542 Thermoactinomyces intermedius 5.29e+3

521 Patulibacter sp. 6.17e+3 543 Litorilinea sp. 5.14e+3

522 Devosia psychrophila 6.17e+3 544 Sphingomonas crusticola 5.14e+3

523 Pelomonas saccharophila 6.17e+3 545 Mesorhizobium camelthorni 5.14e+3

524 Longilinea sp. 6.17e+3 546 Albidiferax ferrireducens 5.14e+3

525 Phenylobacterium aquaticum 6.17e+3 547 Blastomonas aquatica 5.14e+3

526 Nitrosotalea devanaterra 6.17e+3 548 Bradyrhizobium japonicum 5.14e+3

527 Mucilaginibacter lappiensis 6.17e+3 549 Rhodococcus opacus 5.14e+3

528 Prauserella sp. 6.17e+3 550 De uviicoccus sp. 5.14e+3

529 Lysinimonas sp. 6.17e+3 551 Sphingomonas mali 5.14e+3

530 Rhodoplanes piscinae 6.17e+3 552 Sphingomonas leidyi 5.14e+3

531 Turneriella parva 6.17e+3 553 Methylopila oligotropha 5.14e+3

532 Pseudomonas putida 5.87e+3 554 Pseudofulvimonas sp. 5.14e+3

533 Streptomyces panayensis 5.82e+3 555 Marinilutecoccus sp. 5.14e+3

534 Labedaea rhizosphaerae 5.65e+3 556 Klebsiella pneumoniae 5.14e+3

535 Dyadobacter sp. 5.65e+3 557 Flavobacterium caeni 4.99e+3

536 Paenisporosarcina quisquiliarum 5.55e+3 558 Flavobacterium qiangtangense 4.99e+3

#DI9000 16
 Dakota Peat and Equipment

Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

559 Yersinia pestis 4.99e+3 581 Flavobacterium fulvum 4.11e+3

560 Emticicia soli 4.80e+3 582 Mahella australiensis 4.11e+3

561 Gemmata massiliana 4.80e+3 583 Pelomonas sp. 4.11e+3

562 Limnobacter sp. 4.80e+3 584 Heliimonas sp. 4.11e+3

563 Mucilaginibacter vulcanisilvae 4.80e+3 585 Terrimonas lutea 4.11e+3

564 Dactylosporangium sp. 4.80e+3 586 Lysobacter gummosus 4.11e+3

565 Beijerinckia indica 4.80e+3 587 Oleomonas sagaranensis 4.11e+3

566 Methyloversatilis discipulorum 4.80e+3 588 Nocardioides lianchengensis 4.11e+3

567 Flavobacterium saccharophilum 4.70e+3 589 Microbacterium oxydans 4.11e+3

568 Flavobacterium terrigena 4.70e+3 590 Methylocaldum gracile 4.11e+3

569 Inhella inkyongensis 4.63e+3 591 Fulvimonas soli 4.11e+3

570 Dyadobacter beijingensis 4.63e+3 592 Dyella japonica 4.11e+3

571 Streptomyces viridis 4.45e+3 593 Chthonobacter sp. 4.11e+3

572 Plani lum meticola 4.45e+3 594 Vitreoscilla liformis 4.11e+3

573 Pedobacter agrisoli 4.11e+3 595 Bauldia consociata 4.11e+3

574 Rhodococcus jostii 4.11e+3 596 Bacteriovorax stolpii 4.11e+3

575 Zavarzinella sp. 4.11e+3 597 Arenimonas sub ava 4.11e+3

576 Pseudomonas chlororaphis 4.11e+3 598 Paracaedibacter sp. 4.11e+3

577 Microvirga sp. 4.11e+3 599 Massilia eurypsychrophila 3.82e+3

578 Polaromonas glacialis 4.11e+3 600 Streptomyces pratens 3.77e+3

579 Aquaspirillum arcticum 4.11e+3 601 Burkholderia multivorans 3.70e+3

580 Parasediminibacterium paludis 4.11e+3 602 Bacillus simplex 3.70e+3

#DI9000 17
 Dakota Peat and Equipment

Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

603 Polaromonas eurypsychrophila 3.60e+3 625 Microbacterium lacus 3.08e+3

604 Rhodopila sp. 3.60e+3 626 Porphyrobacter sp. 3.08e+3

605 Aquabacterium citratiphilum 3.60e+3 627 Sphingomonas melonis 3.08e+3

606 Pedobacter rhizosphaerae 3.60e+3 628 Sphingomonas phyllosphaerae 3.08e+3

607 Microvirga ossetica 3.60e+3 629 Mycolicibacterium hassiacum 3.08e+3

608 Telmatocola sp. 3.60e+3 630 Aeromicrobium fastidiosum 3.08e+3

609 Flavobacterium pectinovorum 3.52e+3 631 Sphingomonas wittichii 3.08e+3

610 Nocardia exalbida 3.43e+3 632 Finniella sp. 3.08e+3

611 Chitinophaga soli 3.43e+3 633 Fluviicola hefeinensis 3.08e+3

612 Chryseobacterium soldanellicola 3.43e+3 634 Phytohabitans sp. 3.08e+3

613 Sterolibacterium sp. 3.43e+3 635 Blastomonas fulva 3.08e+3

614 Pilimelia columellifera 3.43e+3 636 Caulobacter profundus 3.08e+3

615 Taibaiella yonginensis 3.43e+3 637 Treponema stenostreptum 3.08e+3

616 Pseudonocardia endophytica 3.43e+3 638 Chitinophaga pinensis 3.08e+3

617 Streptomyces lopnurensis 3.43e+3 639 Thermobaculum terrenum 3.08e+3

618 Duganella sp. 3.43e+3 640 Rhodoplanes roseus 3.08e+3

619 Verticiella sp. 3.43e+3 641 Roseisolibacter sp. 3.08e+3

620 Aminobacter aminovorans 3.43e+3 642 Singulisphaera acidiphila 3.08e+3

621 Ensifer adhaerens 3.29e+3 643 Thermocrispum municipale 3.08e+3

622 Flavobacterium cauense 3.23e+3 644 Aetherobacter sp. 3.08e+3

623 Cereibacter changlensis 3.08e+3 645 Planococcus antarcticus 2.97e+3

624 Sphingomonas humi 3.08e+3 646 Clostridium sp. 2.97e+3

#DI9000 18
 Dakota Peat and Equipment

Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

647 Clostridium estertheticum 2.96e+3 669 Roseomonas vinacea 2.47e+3

648 Actinoallomurus bryophytorum 2.88e+3 670 Pseudomonas uorescens 2.40e+3

649 Paraburkholderia phymatum 2.74e+3 671 Massilia violacea 2.35e+3

650 Desulfosporosinus sp. 2.74e+3 672 Geobacillus sp. 2.26e+3

651 Actinoplanes sp. 2.74e+3 673 Aggregicoccus sp. 2.06e+3

652 Terrimonas terrae 2.74e+3 674 Modestobacter multiseptatus 2.06e+3

653 Deinococcus roseus 2.74e+3 675 Brevifollis sp. 2.06e+3

654 Novosphingobium barchaimii 2.74e+3 676 Aurantisolimonas haloimpatiens 2.06e+3

655 Salinarimonas sp. 2.74e+3 677 Uliginosibacterium sediminicola 2.06e+3

656 Pseudonocardia eucalypti 2.74e+3 678 Exiguobacterium sp. 2.06e+3

657 Allocatelliglobosispora scoriae 2.74e+3 679 Pseudomonas alcaligenes 2.06e+3

658 Flavitalea gansuensis 2.74e+3 680 Pseudonocardia spinosispora 2.06e+3

659 Sphingomonas sanxanigenens 2.74e+3 681 Neobacillus niacini 2.06e+3

660 Sulfurovum sp. 2.74e+3 682 Syntrophomonas sp. 2.06e+3

661 Demequina sp. 2.74e+3 683 Cellvibrio mixtus 2.06e+3

662 Rubritepida sp. 2.57e+3 684 Methylobacterium dankookense 2.06e+3

663 Pontibacter sp. 2.57e+3 685 Chryseobacterium formosense 2.06e+3

664 Microvirga guangxiensis 2.57e+3 686 Dyadobacter soli 2.06e+3

665 Solitalea sp. 2.57e+3 687 Pedobacter bauzanensis 2.06e+3

666 Chitinimonas sp. 2.57e+3 688 Stenotrophomonas panacihumi 2.06e+3

667 Nibribacter koreensis 2.57e+3 689 Byssovorax sp. 2.06e+3

668 Roseomonas arctica 2.47e+3 690 Ru bacter tibetensis 2.06e+3

#DI9000 19
 Dakota Peat and Equipment

Results are expressed in 'cells per gram' or 'cells per milliliter', depending on the sample being a solid or liquid.

691 Belnapia sp. 2.06e+3 713 Skermanella aerolata 1.37e+3

692 Aquabacterium sp. 2.06e+3 714 Nitrospirillum sp. 1.23e+3

693 Kineosporia sp. 2.06e+3 715 Roseomonas aquatica 1.23e+3

694 Tundrisphaera lichenicola 2.06e+3 716 Sporosarcina psychrophila 1.23e+3

695 Actinophytocola xanthii 2.06e+3 717 Streptomyces thermoautotrophicus 1.23e+3

696 Minicystis sp. 2.06e+3 718 Roseomonas frigidaquae 1.23e+3

697 Dyadobacter hamtensis 2.06e+3 719 Roseomonas suffusca 1.23e+3

698 Bacillus acidiceler 1.85e+3 720 Thermoactinomyces daqus 1.17e+3

699 Exiguobacterium undae 1.83e+3 721 Flavobacterium aquatile 1.17e+3

700 Bacillus megaterium 1.74e+3 722 Flavobacterium banpakuense 1.17e+3

701 Streptomyces luteogriseus 1.71e+3 723 Flavobacterium suzhouense 1.17e+3

702 Mobilitalea sp. 1.64e+3 724 Acinetobacter sp. 1.03e+3

703 Roseomonas lacus 1.64e+3 725 Pelosinus sp. 9.13e+2

704 Inquilinus limosus 1.54e+3 726 Paenibacillus endophyticus 8.22e+2

705 Perlucidibaca sp. 1.54e+3 727 Bacillus sp. 8.22e+2

706 Cohnella sp. 1.54e+3 728 Clostridium vincentii 6.17e+2

707 Rhodovarius sp. 1.54e+3 729 Aneurinibacillus aneurinilyticus 6.05e+2

708 Flavobacterium frigidimaris 1.47e+3 730 Peribacillus psychrosaccharolyticus 4.74e+2

709 Paenibacillus sp. 1.44e+3

710 Rummeliibacillus sp. 1.37e+3

711 Streptomyces kebangsaanensis 1.37e+3

712 Acinetobacter kookii 1.37e+3

#DI9000 20
 Dakota Peat and Equipment

Notes
Species present in the CDFA Approved Microorganisms List

Species belonging to a genus present in the CDFA Approved

Microorganisms List

#DI9000 8
 Dakota Peat and Equipment

APPENDIX
Fungi Quantification 2.0
The Next-Gen sequencing in combination with the addition of a known quantity spike-in enables the knowledge of the total microbial load in a sample.
The present analysis relies on the application of a spike-in of our synthetic proprietary DNA sequence in known quantities into crude samples. After the
sequencing and data processing, the relative abundance of the exogenous spike-in allows us to extrapolate the original absolute quantity of the ITS copies
of the sample species. NOTE: number of cells cannot be provided for Fungi due to many factors including very limited knowledge of ITS copies per
genome, ploidy variations, pluricellularity, etc.
Results are expressed in 'ITS copies per gram' or 'ITS copies per milliliter', depending on the sample being a solid or liquid.

# Genus & Species Percentage Copies # Genus & Species Percentage Copies

1 Conocybe apala 57.56% 602,513 18 Spumella sp. 0.4819% 5,047

2 Conocybe pallidospora 15.82% 165,607 19 Penicillium brasilianum 0.4532% 4,747

3 Pythium sp. 3.20% 33,527 20 Pluteus cinereofuscus 0.3901% 4,087

4 Chloridella sp. 2.04% 21,380 21 Exophiala equina 0.3882% 4,067

5 Globisporangium hypogynum 1.70% 17,813 22 Mortierella exigua 0.3844% 4,027

6 Curvularia verruculosa 1.50% 15,713 23 Chrysosporium synchronum 0.3786% 3,967

7 Mortierella sp. 1.40% 14,653 24 Pseudeurotium hygrophilum 0.3576% 3,740

8 Globisporangium sylvaticum 1.26% 13,173 25 Coniothyrium crepinianum 0.3021% 3,160

9 Aspergillus fumigatus 1.11% 11,613 26 Pyrenochaetopsis leptospora 0.2926% 3,060

10 Mortierella amoeboidea 0.8185% 8,567 27 Pezoloma ericae 0.2907% 3,040

11 Mortierella elongata 0.7363% 7,707 28 Pezicula brunnea 0.2869% 3,000

12 Kazachstania humilis 0.6406% 6,707 29 Leptosphaeria sp. 0.2849% 2,980

13 Pseudogymnoascus roseus 0.5814% 6,087 30 Microdochium bolleyi 0.2582% 2,700

14 Penicillium pimiteouiense 0.5756% 6,027 31 Vishniacozyma victoriae 0.2218% 2,320

15 Chrysosporium pseudomerdari … 0.5163% 5,407 32 Conocybe sp. 0.2199% 2,300

16 Pythium vanterpoolii 0.4857% 5,087 33 Alternaria eichhorniae 0.2180% 2,280

17 Mortierella alpina 0.4857% 5,087 34 Hannaella zeae 0.2161% 2,260

32/12-0009ID-RP

#DI9000 22
 Dakota Peat and Equipment

Relative Fungal Abundance

Results are expressed in 'ITS copies per gram' or 'ITS copies per milliliter', depending on the sample being a solid or liquid.

# Genus & Species Percentage Copies # Genus & Species Percentage Copies

35 Penicillium sp. 0.2161% 2,260 55 Phialocephala sp. 0.0937% 980

36 Mortierella gamsii 0.2046% 2,140 56 Arachnomyces pilosus 0.0918% 960

37 Sclerotinia sclerotiorum 0.1931% 2,020 57 Coniosporium apollinis 0.0822% 860

38 Scedosporium boydii 0.1893% 1,980 58 Bolbitius lacteus 0.0803% 840

39 Phallus rugulosus 0.1874% 1,960 59 Epicoccum nigrum 0.0803% 840

40 Psathyrella scatophila 0.1817% 1,900 60 Pseudogymnoascus sp. 0.0727% 760

41 Apiotrichum veenhuisii 0.1492% 1,560 61 Ganoderma applanatum 0.0708% 740

42 Tricellula aurantiaca 0.1473% 1,540 62 Conocybe zeylanica 0.0650% 680

43 Arachnomyces gracilis 0.1358% 1,420 63 Chloridium aseptatum 0.0650% 680

44 Fusarium oxysporum 0.1300% 1,360 64 Aspergillus penicillioides 0.0631% 660

45 Apiotrichum loubieri 0.1300% 1,360 65 Penicillium melinii 0.0478% 500

46 Eustigmatos sp. 0.1243% 1,300 66 Lecythophora sp. 0.0459% 480

47 Humicola olivacea 0.1243% 1,300 67 Microscypha sp. 0.0440% 460

48 Alternaria infectoria 0.1243% 1,300 68 Scopulariopsis sp. 0.0440% 460

49 Cladosporium herbarum 0.1109% 1,160 69 Pedospumella sp. 0.0344% 360

50 Trichocladium opacum 0.1090% 1,140 70 Conocybe dunensis 0.0344% 360

51 Penicillium levitum 0.1052% 1,100 71 Conocybe velutipes 0.0325% 340

52 Didymella exigua 0.0994% 1,040 72 Circinella chinensis 0.0306% 320

53 Phoma sp. 0.0975% 1,020 73 Clitopilus sp. 0.0287% 300

54 Petrakia sp. 0.0937% 980 74 Aspergillus terreus 0.0287% 300

32/22-0009ID-RP

#DI9000 23
 Dakota Peat and Equipment

Relative Fungal Abundance

Results are expressed in 'ITS copies per gram' or 'ITS copies per milliliter', depending on the sample being a solid or liquid.

# Genus & Species Percentage Copies # Genus & Species Percentage Copies

75 Mortierella globulifera 0.0268% 280

76 Aspergillus chlamydosporus 0.0229% 240

77 Keithomyces carneus 0.0210% 220

78 Onychocola glareosa 0.0210% 220

79 Trichoderma harzianum 0.0191% 200

80 Cephalosporium gramineum 0.0191% 200

81 Mortierella antarctica 0.0191% 200

Notes
Species belonging to a genus present in the CDFA Approved
Microorganisms List

Species present in the CDFA Approved Microorganisms List

32/32-0009ID-RP

#DI9000 24
 DAKOTA_PEAT Dakota Peat and Equipment

CO PYR I G HT ® 2 0 2 4 BI O M E M A K E R S I N C. A L L R I G H TS R E S E R V E D .

For research purposes only. Reference herein to any speci c commercial product, process, or service by its trade name, trademark, manufacturer, or
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