Encept Glycine ; all amino acid > -

optically active

Except Proline ; all amino acid have PNH2 group .

essential and Semiessential Histidine

2
Arginine
>
⑧Essential
-

10 Non ,
.

15 neutral 2 Acidic ,
3 Basic Amino acids
,

(R-CH-C001) (Only e
Amino Acids written
he
Neutral
R
·

④ Glycine
O-ry O Aspargine
O
G CH
1
-

Ptophan /- - - 1
H- 11T
I ·
E
(Asn) HCN-C-C-
(Gly) (rb)
② Alanine
-
- PhunyeAlanine Ph-cH- GlutamineNN--c-c- O

(Ala) (Phe) (Gen)

③ Valine
Me
I
⑧ Cysteine Hs-CH-
⑬ Serine
HO-CH2-
Me-C
* (cys)
-

(Val) (ser)

④ Leucine
Me
④Methionine OThree
is C
me-c mes-c-c-
Threonine I
*
C-
-

HO-C
(Met)
-

(Lea) (Thu)

⑤ O
⑳Grove
co
Me
15 Tymes
e ·i
w

Xi
Isoleucine Me-c-c-
I
②
T
Ho-1& C-
rosine
(Ile)
coon
Ty
(Tys]
·
Acidic Amino Acid ·
Basic Amino Acids

Q Aspartic Acid HOOC-CH, - ① Histidine i - CH

-
I
(Asp) (His) N

NH

② Glutamic Acid
HOOC -C-C =
② Arginine 19

MN-C-NH-C-C-C-
(Glu) (Arg)

·
Essential Amino Acids & Lystine HN-C-C-C-C-

(Lys)
three -
Isolated Lyst
Thereasing Isoleucine
Lystine

Phen
Me va Leu
of Tryp
Methionine Valine
Tryptophan Phenyl
Leucine Alanine
 X-Amino Acids
Protein : Natural Polymer of
Insulin is the smallest protein (having 51 amino acids)

① Primary Structure : Has only peptide bonds .

Linear chain in specific sequence .

*
11 d
H-bond + Peptide
and
② NH F
N-
Structure :
Secondary Bond
- -

Wil 1 antill
Two
191

types
-
our c-NH-C-NH-C

types
:

-Helin and -pleated sheet

E
-n
a
& TertiaryStructure Formed due to
folding of secondary stc .

Contains peptide bonds ,

H-bonds , di-Suphide bond -S-s7
with Vanderwaal and electrostatic FOA
along
-

It is
of two types
:
&
④ Fibrous (water insolb) B Globular (water soluble)

# FibrelikeParallelchaie
n Formed by coiling ,
has

spherical shape .

bond- Insulin , Albumin -

Keratin (in hair , wool silk)

-

↳
egg protein
,
.

Myosin (in muscles)

④ Guanternary Structure formed
by two or more polypeptide chains with spatial
arrangement. -
Non
(fet) , Chlorophyll (Mg>
-
2) +

En :
Heamoglobin part .

Denaturation /4 Maybe reuw or

of Protein
% :
3 Heat of
>
1 structure
Structure non-revo
chem treatment
.

of protein Brilling of egg is

irrev

↑
↓
Amino Acids and Proteins H20
· Test of . -
N
-
..
- ↓
Violet
& Pirate Test
tip-it-n - + 2
(ag CusO4)
.
> -

/
N
>
- Cuz

↑
:
N Complem

O NH H20
Given by -"-NH- ,
-NH2 ,
-

CH2-NH2 ,
If

-C-NH2 -C-wiL ,
Si

(Test of A acid and protien with free We go t

2
Ninhydrin Test
O

MIN-cH-COOH + 2101
/-
1
O "on xil i
-
- > 1 of + RCHO + cost
Lot
OH -
N-

-
-

- H O &-
,

d
> Purple-blue

>
-
Prolein (2"Amine) + Nin-hydrin > Yellow Color .
3 Xanthoproteic Test (Test of aromatic
amino acid)
= Gives color
>
-

Nitration rek" yellow

Test amino acid

↳
Sakaguchi Test > =

of arginine .

NaOBr
> Red Coloured Complex
X-Napthol
.

2
Arginine +

= Millon's Test (Test of soluble protein like Tyrosine Amino acid) .

Millon's
Tyrosine > Reddish-Brown Complex .

Reagent
SANGER's Method of Sequencing of proteins
.

YO2 amino acid from N-terminal

/-- F cuts the

101
&N
>
-
> Sanger's Reagent