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MULTIBLE CHOICE

QUESTIONS
Q1. Correct sentence regarding prokaryotes?

a. Nucleolus is present

b. Cell membrane does not contain sterols except in Mycoplasma

c. Cellular organelles like mitochondria are present

d. Extrachromosomal DNA is found in mitochondria

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Q2. Who is the Father of Microbiology?

a. Antonie Philips van Leeuwenhoek

b. Edward Jenner

c. Louis Pasteur

d. Joseph Lister

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Questions:

Q3. Electrons are used as a source of illumination in?

a. Light microscope

b. Phase contrast microscope

c. Electron microscope

d. Fluorescence microscope

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Questions (Cont..) :

Q4. Scanning probe technology is used in ?

a. Light microscope

b. Phase contrast microscope

c. Electron microscope

d. Atomic force microscope

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Questions (Cont..) :

Q5. Microscope used for studying microbial motility?

a. Light microscope

b. Phase contrast microscope

c. Electron microscope

d. Atomic force microscope

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Questions (Cont..) :

Q6 Resolution power of microscope is improved by using?

a. Oil

b. Stain

c. Lenses

d. Condenser

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Questions:
Q7. Most recent taxonomic classification of bacteria is based on?

a. Haeckel – 3 kingdom classification

b. Copeland – 4 kingdom classification

c. Whittaker - 5 kingdom classification

d. Cavalier and Smith’s - 6 kingdom classification

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Questions (Cont..):
Q8. Which of the following is correct sentence?

a. Adansonian classification - represents a branching tree-like arrangement

b. Phylogenetic classification - genetic relatedness of different organisms

c. In binomial nomenclature - scientific name of bacteria comprises of both genus


and species names.

d. Both genus and species should be written in capitals.

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Questions:
Q9. Bamboo stick appearance arrangement is characteristic of:

a. Staphylococcus

b. Streptococcus

c. Corynebacterium diphtheriae

d. Bacillus anthracis

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Questions:

Q10. Bacterial capsule can be best demonstrated by:

a. Gram staining

b. Acid-fast staining

c. Negative staining

d. Albert staining

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Questions:

Q11. Lipopolysaccharide is a component of cell wall of:

a. Gram-positive bacteria

b. Gram-negative bacteria

c. Virus

d. Fungi

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Questions:

Q12. Bacterial structure involved in respiration is:

a. Ribosome

b. Pili

c. Mesosome

d. Flagella

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Questions:
Q13. Bacteria are uniformly stained in which of the following phase of growth
curve?

a. Lag phase

b. Log phase

c. Stationary phase

d. Declining phase
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Questions:
▰ Q14. Agar concentration required to prepare semisolid media:

a. 0.1%

b. 0.5%

c. 2%

d. 6%

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Questions:
▰ Q15. Type of specimen collected for URTI:

a. Blood

b. Throat swab

c. Sputum

d. BAL

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Questions:
▰ Q16. Sulfuric acid (%) needed for decolorization of M. tuberculosis:

a. 25%
b. 5%

c. 1%

d. 0.5%

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Questions:
▰ Q17. Examples of enriched media are all except:

a. Blood agar

b. Loeffler’s serum slope

c. Blood culture media

d. MacConkey agar

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Questions:
▰ Q18. Automated blood culture bottles contains:

a. Selenite F broth

b. Tryptic soy broth

c. Peptone water broth

d. BHI broth

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Questions:

▰ Q19. Which of the following is not an equipment for automated blood


culture:

a. BacT/ALERT

b. VITEK2

c. BACTEC

d. VersaTREK
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Questions:

▰ Q20. BacT/ALERT - principle is based on::

a. Calorimetric detection

b. Fluorescent detection

c. Detection of change of pressure

d. All the above

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Questions:

▰ Q21. Three basic steps of PCR cycle involves all, except:

a. Denaturation

b. Amplification

c. Extension

d. Gel documentation

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Questions:

▰ Q22. Mechanism of direct transfer of free DNA:

a. Transformation

b. Conjugation

c. Transduction

d. Transposition

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Questions:

▰ Q23. Plasmids have following functions except:

a. Transposition

b. Conjugation

c. Resistance

d. Virulence

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Questions:
▰ Q24. Griffith experiment on mice using pneumococci strains provides the
direct evidence of:

a. Transformation

b. Transduction

c. Conjugation

d. Transposition
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Questions:

▰ Q25. Horizontal transmission of ‘R’ factor is by :

a. Transformation

b. Transduction

c. Conjugation

d. Fusion

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Questions:
▰ Q26. 1st line treatment when S.aureus is isolated in a specimen:

a. Vancomycin

b. Doxycycline

c. Cloxacillin

d. Daptomycin

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Questions:
▰ Q27. S. pyogenes can be differentiated from S. agalactiae by:

a. Bacitracin resistant

b. CAMP test negative

c. Alpha hemolytic colonies on blood agar

d. Gram-positive cocci n clusters

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Questions:

▰ Q28. Selective media for Neisseria gonorrhoeae:

a. LJ media

b. Ashdowns media

c. V-R media

d. Thayer martin media

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Questions:
▰ Q29. Causative agent of Q-fever:

a. Bartonella

b. Coxiella burnetii

c. Ehrlichia

d. R. prowazekii

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Questions:
▰ Q30. Causative agent of pseudomembranous colitis:

a. Clostridium tetani

b. Clostridium botulinum

c. Clostridioides difficile

d. Clostridium perfringens

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Questions:
▰ Q31. Which of the following virus is enveloped:

a. Poliovirus

b. Adenovirus

c. Herpesvirus

d. Parvovirus B19

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Questions:
▰ Q32. All of the following viruses are transmitted by respiratory route,
except:

a. Influenza virus

b. Rotavirus

c. RSV

d. Rhinovirus
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Questions:
▰ Q33. Which of the following vaccine is a killed vaccine:

a. Mumps vaccine

b. Measles vaccine

c. Rubella vaccine

d. IPV

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Questions:
▰ Q34. The largest virus in size is:

a. Herpes simplex virus

b. Hepatitis B virus

c. Poxvirus

d. Adenovirus

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Questions:
▰ Q35 Which of the following DNA virus is single stranded:

a. Parvovirus

b. Hepatitis B virus

c. Poxvirus

d. Adenovirus

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Questions:
▰ Q36. Human fibroblast cell line is used for cultivation of:

a. Measles

b. CMV

c. Poliovirus

d. Adenovirus

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Questions:
▰ Q37. Host harboring adult or sexual stage of a parasite is called:

a. Definitive host

b. Intermediate host

c. Reservoir host

d. None of the above

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Questions:
▰ Q38. Which of the following parasite is transmitted by dog:

a. Taenia saginata

b. Hymenolepis nana

c. Echinococcus granulosus

d. Diphyllobothrium latum

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Questions:
▰ Q39. Flotation technique is useful for the detection of:

a. Fertilised eggs of Ascaris

b. Larva of Strongyloides

c. Taenia eggs

d. Operculated eggs of trematodes

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Questions:
▰ Q40. Advantages of saline mount are all except:

a. Useful in the detection of trophozoites and cysts of protozoa

b. Nuclear details of cysts and helminthic eggs are better visualised

c. Motility of trophozoites can be seen in acute infection

d. Bile staining property can be appreciated

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