Fluorescence spectrometer

:

-:
Spectrofluorimeter ) Fluorescence spectrometer .(1-9

) :(1-9 Fluorescence spectrometer

-
absorption
excitation

237

ground state
emission
fluorescence

) .( 2 -9

) :(2 -9

238

-:
aflatoxins riboflavin
. polynuclear aromatic hydrocarbons
.
-: aliphatic
amines acids

fluorescent

derivatives fluorophore group

waste water
mycotoxins

.

.

) (3 -9

electronic ground state (E0 )electronic excited state (E1

)E0 ( v0, v1, v2, v3

) E1 (v0, v1, v2, v3 .

.

239

) :(3 -9

.


)E1 (v0, v1, v2

) E0 (v0, v1, v2 .

240

.

.

fluorescent light
excitation light emission
spectrum excitation spectrum
.

: ) (4-9 :

Energy Source

Excitation monochromator

Sample cell

Emission monochromator

Detector

Recorder

241

) :(4 -9
A general schematic of a fluorimeter

:
:
.1

: Lasers

high irradiance
very narrow wavelength interval nm 0.01

.
.2 Mercury lamp : Xenon arcs lamp
line

nm 800-300 nm 200

242

-: filter fluorometers
spectrofluorometers -:
Filter Fluorometers
incident light fluorescent light
.diffraction grating spectrofluo
rimeters .

diffraction grating collimated light

.
anisotropy measurements
excitation monochromator emission
monochromator

stray light
.

:

excitation wavelength
emission wavelength
narrow band
) dual filter fluoromiter .(5-9

243

Simple filter fluorimeter

Dual filter fluorimeter

) :(5-9

single-channeled
. multi-channeled

.
.
dual
monochromators continuous excitation light
source .


. emission monochromator

244

emission filter or monochromator
excitation monochromator


.

:

Fluorophore
.

photodecomposition
.

scattering of light
.

peak

3600

cm1 .
excitation source
incident
light fluorescence .
fluorescent light

90
.

245