Code :R7412311 1

IV B.Tech I Semester(R07)Regular Examinations, November/December 2010

METABOLIC ENGINEERING
(Biotechnology)
Time: 3 hours Max Marks: 80
Answer any FIVE questions
All questions carry equal marks

?????

1. Give a basic concept and overview of metabolic engineering.

2. Give a detail account of amino acid synthesis.
3. Write a detail note on major pathways for secondary metabolic synthesis and the effect of precursors on
metabolic fluxes.
4. What do you mean by strain selection? Write note on genetic improvement of strains for production of
enzymes.
5. Give an account of manipulation of metabolic pathway to improve fermentation process.
6. Give an overview of integration of anabolism and catabolism in a biosynthetic pathway.
7. Write on the role of bioinformatics for modeling of metabolic pathways.
8. Give an overview on the applications of metabolic engineering in phamachemicals.

?????
Code :R7412311 2
IV B.Tech I Semester(R07)Regular Examinations, November/December 2010
METABOLIC ENGINEERING
(Biotechnology)
Time: 3 hours Max Marks: 80
Answer any FIVE questions
All questions carry equal marks

?????

1. Give an overview of cellular metabolism and write on different models for reactions in the cell.
2. Write about amino acid synthesis and its regulation at enzyme level.
3. Write short notes on:
(a) Prophase
(b) Idiophase
4. Define bioconversion. What are the factors affecting bioconversions, and its applications.
5. Write short notes on:
(a) Feed back repression
(b) Catabolic repression
6. Write short notes on:
(a) Material balance
(b) Equilibrium reaction.
7. Write about the application of bioinformatics in the analysis of metabolic control and the structure.
8. Describe in detail on the applications of metabolic engineering in chemical bioprocess and food technology.

?????
Code :R7412311 3
IV B.Tech I Semester(R07)Regular Examinations, November/December 2010
METABOLIC ENGINEERING
(Biotechnology)
Time: 3 hours Max Marks: 80
Answer any FIVE questions
All questions carry equal marks

?????

1. Write an detail about Jacob and Monod model and regulation in expression of a gene cascade.
2. Write about the mechanism of the after action of feedback regulation by restricting accumulation of end
product .
3. Describe how catabolic regulation bypasses the control of secondary metabolism with suitable example.
4. Write shorts on the following:
(a) Gene dosage
(b) Product inhibition
5. What are the check points for the optimization and control of metabolic activities?
6. Give an account of experimental determination method of flux distribution, analysis and its application.
7. Describe how bioinformation can be useful in elucidating metabolic networks.
8. Write about applications of metabolic engineering in agriculture.

?????
Code :R7412311 4
IV B.Tech I Semester(R07)Regular Examinations, November/December 2010
METABOLIC ENGINEERING
(Biotechnology)
Time: 3 hours Max Marks: 80
Answer any FIVE questions
All questions carry equal marks

?????

1. Write short notes on the following:

(a) Iso-enzymes
(b) Feed back regulation
2. Give an overview of synthesis of primary metabolites.
3. Write in detail about the products of secondary metabolism and applications of secondary metabolites.
4. Write about mixed and sequential bioconversions.
5. Give a strategies by which the modification of existing or introduction of entirely new metabolic pathway
is possible.
6. Write in detail about the thermodynamics of cellular processes with suitable biosynthetic reaction.
7. Give a detail account of metabolic pathway synthesis algorithms.
8. Write how metabolic engineering will be beneficial for environmental bioremediation with suitable ex-
amples.

?????