MICROBIOLOGY 1

Practical examination 2
BIOCHEMICAL TESTING
ENTEROBACTERIACEA
Prepared by:
Micro1 Cluster
FEU-Manila
1st sem 2020-2021
Instructions
• Encode all answers in the designated answer
sheet.
• Submit this practical examination on or before
the designated deadline.
PLATE 1

Identify the correct results for

Each tube of TSI and indicate which
type of sugar was/were utilized for each.

1.

2.

3.

4.

5.

1. 2. 3. 4. 5.
PLATE 2

Give the appropriate reading of

Each biochemical test:

6.

7.

8.

9. Motility =
Indole =
Ornithine =

10. What would be the most possible

Organism that will exhibit these results?
(Genus level only)

6. 7. 8. 9.
PLATE 3

11. Describe results in Gram Stain.

12. Describe colonies in MacConkey agar.

13. What are the 2 possible organisms under Enterobacteriaceae?

(Genus only)

14. What would the organism be if it was Urease positive?

15. What 2 organisms would it be if it were indole positive?

(genus and specie)
 PLATE 4

16. Describe colonies in BAP.

17. Describe oxidase test result.

18. Can this organism be suspected as Enterobacteriaceae?

19. Would this colony be lactose or nonlactose

fermenting in MacConkey agar?
Oxidase test

20. Give the result of the suspected organism

for the following biochemical test.
a. Indole =
b. TSI =
c. Urease =
PLATE 5

21. True or False: This belongs under the family of

Enterobacteriacea?

22. Based on the colonial appearance; what common

hospital isolate is highly suspected?

Oxidase test
 PLATE 6

A patient presented with diarrhea and stomach pains. She cannot remember anything
in particular that she ate that may have caused her condition. She submitted stool sample
to the laboratory for microbiology work up. The following were the results.

23. What is the first biochemical test to

Perform to identify if this organism is
Under Enterobacteriacea and what is
The result?

Gram stain
24. Based on the patient history, if this organism
Is Enterobacteriacea, what would be the most
Likely organism to suspect? (Genus only)

25. What could be another biochemical test

(other than TSI) that would help confirm
your answer in no.24 and what is the result?
MacConkey Agar TSI
 PLATE 7

MacConkey Agar
Provided this organism is Enterobacteriacea,
Supply the results for the following:

26. Growth in MacConkey

27. Oxidase =

28. TSI =

TSI SIM 29. Sulfide =

Indole =
Motility =

30. Highly suspected organism:

END