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The history and scope of food microbiology has been classified during various time
periods and these have been stated as follows: (Oberg, 2012).
7000 BC – Evidence that the Babylonians manufactured beer (fermentation). Wine
appeared in about 3500 BC. In early civilizations and even in the present world in
underdeveloped countries, where modern sanitation is lacking, alcoholic beverages like beer
and wine were much safe to consume than the local water supply. The reason being, the water
was often contaminated with intestinal micro-organisms that caused cholera, dysentery and
other serious diseases.
Bacteria
Bacteria are a large sphere of prokaryotic micro-organisms. Bacteria are present in
most habitats on Earth, growing in soil, acidic hot springs, radioactive waste water, organic
matter and live bodies of plants and animals. Bacteria have different types of shapes and
sizes. Bacterial cells are about one tenth the size of eukaryotic cells and 0.5–5.0 micrometres
in length. Most bacterial species are either spherical, called cocci or rod-shaped, called
bacilli. Some rod-shaped bacteria are slightly curved called vibrio or comma-shaped. Many
bacterial species exist as single cells and associate in characteristic patterns such as, form
pairs called diploids, form chains, and group together in clusters. Bacteria can also be
elongated to form filaments (Module 1, n.d.).
The bacterial cell is encircled by cell membrane, which encompasses the contents of
the cell and acts as a barrier to hold nutrients, proteins and other crucial components of the
cytoplasm within the cell. They lack a true nucleus, mitochondria, chloroplasts, Golgi
apparatus and endoplasmic reticulum. Most bacteria do not have a membrane-bound nucleus,
and their genetic material is normally a single circular chromosome located in the cytoplasm
in an irregularly shaped body called the nucleoid. The nucleoid contains the chromosome
with associated proteins and RNA. The bacteria contain ribosomes for the production of
proteins but different from those of eukaryotes and Archaea. Some bacteria produce
intracellular nutrient storage granules, such as glycogen, polyphosphate, sulfur or
polyhydroxyalkanoates. These granules enable bacteria to store compounds for later use
(Module 1, n.d.).
Molds
Molds are multicellular micro-organisms with mycelial (filamentous) morphology.
These microbes are also characterized by their exhibition of a variety of colours and are
generally recognized by their mild or incoherent, cotton like appearance. Molds can develop
numerous tiny spores that are found in the air and can be spread by air currents. These spores
can produce new mold growth, if they are transferred to a location that has conditions
conducive to germination. Molds normally withstand greater fluctuation in pH than bacteria
and yeasts and can frequently tolerate more temperature fluctuation (Unit I, n.d.).
Although molds thrive best at or near a pH of 7.0, a pH range of 2.0 to 8.0 can be
tolerated, even though an acid to neutral pH is preferred. Molds flourish better at ambient
temperature than in a colder environment, even though growth can occur below 0°C.
Although mold growth is optimal at a water activity (Aw) of approximately 0.85, and growth
can take place below 0.80. At an Aw of 0.90 or higher, bacteria and yeasts grow more
effectively and normally utilize available nutrients for growth at the expense of molds. When
the Aw goes below 0.90, molds grow more effectively. That is why foodstuffs, such as
pastries, cheese, and nuts, that are low in moisture content are more likely to spoil from mold
growth (Unit I, n.d.).
Yeasts
Yeasts are generally unicellular and differ from bacteria in their large cell size
and morphology, and because they produce buds during the process of reproduction by
division. Like molds, yeasts can spread through the air, or other means, and alight on the
surface of foodstuffs. Yeast colonies are normally moist or slimy in appearance and creamy
white coloured. Yeasts prefer an Aw of 0.90 - 0.94, but can grow below 0.90. These micro-
organisms grow best in the intermediate acid range, pH from 4.0 to 4.5. Food that is
contaminated with yeasts will have a slightly fruity odour on a frequent basis (Unit I, n.d.).
Viruses
Viruses are 10-450 nm in size, cannot reproduce without a living host, attack
only susceptible host cell lines, infect plants, animals, and bacteria, and have the ability to
produce specific diseases in explicit hosts. Transmission occurs in foods, water and air.
Viruses that infect bacteria are called bacteriophages. Viruses are included in the order
Virales. Viruses are too small to be visualized with an ordinary compound microscope. Only
after the electron microscope was developed, the direct observation of viruses was possible.
Viruses consist of a DNA or RNA core surrounded by a protein coat. Because they lack all
the apparatus for normal cellular metabolism, they must utilize the cellular machinery of the
host cell in order to grow and divide. Once they invade a host cell, however, viruses can
multiply rapidly (Unit I, n.d.).
Protozoa
Protozoa are parasitic and animal-like protists because of their motility. Their sizes
range from 10 to 52 micro-meters. They move by flagella, hair-like structures called ciliaand
and foot-like structures called pseudopodia. Protozoa absorb food through their cell
membranes e.g., amoebas, surround food and consume it. All protozoa digest their food in
stomach-like compartments called vacuoles. Protozoa can reproduce by binary fission or
multiple fission. Some protozoa reproduce sexually, some asexually, while some use a
combination. They cause many diseases in humans such as, malaria, amoebiasis and
leishmaniasis (Module 1, n.d.).
Fungi
Fungi are eukaryotic organisms that include yeasts, molds and mushrooms. They are
non-photosynthetic and do not contain chlorophyll pigments. Most of them are multi-cellular
and some are uni-cellular e.g. yeast. They are non-motile and lack true leaves, roots and
stems. Fungi need warm and moist places to grow. They are found mainly in moist foods,
damp tree barks, and wet bathroom tiles. Fungi are heterotrophs that feed by absorption. They
absorb small organic molecules from the surrounding medium. The enzymes and hydrolytic
enzymes secreted by the fungus break down food outside its body into simpler compounds
that the fungus can absorb and use. The absorptive mode of nutrition is associated with the
ecological roles of fungi as decomposers, parasites, and mutualistic symbionts (Module 1,
n.d.).
Saprobic fungi absorb nutrients from non-living organisms. Parasitic fungi absorb
nutrients from the cells of living hosts. The fungal cells contain membrane-bound nuclei with
chromosomes that contain DNA with non-coding regions called introns and coding regions
called exons. They also possess membrane-bound cytoplasmic organelles, such as
mitochondria, sterol-containing membranes, and ribosomes of 80S type. They have soluble
carbohydrates and storage compounds, including sugar alcohols, disaccharides, and
polysaccharides. Fungi lack chloroplasts and are heterotrophic organisms, requiring
preformed organic compounds as energy sources (Module 1, n.d.).
Fungi possess a cell wall and vacuoles. They reproduce by both sexual and asexual
means and produce spores. They have haploid nuclei. The cells of most fungi grow as
tubular, elongated, and thread-like structures are called hyphae which may contain numerous
nuclei. Some species grow as single-celled yeasts that reproduce by budding or binary
fission. The fungal cell wall is composed of glucans and chitin. Most fungi grow as hyphae,
which are cylindrical, thread-like structures 2–10 μm in diameter and up to several
centimeters in length. Hyphae grow at their tips; new hyphae are typically formed by a
process called branching, or growing hyphal tips bifurcate giving rise to two parallel-growing
hyphae. Hyphae can be either septate or coenocytic, septate hyphae are divided into
compartments separated by cross walls, with each compartment containing one or more
nuclei; coenocytic hyphae are not compartmentalized. Fungal reproduction is multifaceted.
They reproduced by both sexually and asexually. A sexual reproduction via vegetative spores
(conidia) or through mycelial fragmentation. Sexual reproduction involves joining of hyphae
is called conjugation, two mating strains with different nuclei form continuous membrane is
known as plasmogamy and sometimes the nuclei are fused is called karyogamy (Module 1,
n.d.).
Primary Sources of Micro-organisms to Food
The primary sources of micro-organisms to food have been stated as follows: (Osho,
n.d.).
Soil and Water - In soil and water, organisms are generally encountered which are
often food-borne bacteria, i.e. Alcaligenes, Bacillus, Citrobacter, Clostridium,
Corynebacterium, Enterobacter, Micrococcus, Proteus, Pseudomonas, Serratia and
Streptomyces among others. For molds, some of the most commonly encountered in soils are
Aspergillus, Rhizopus, Penicillium, Trichothecium, Botrytis, Fusarium, and others. In the
case of yeast, a large number of yeast genera are found in the soil, but their number are
generally low in water.
Plants and Plant Products - Bacteria often found to be associated with plants and
plant products are, Acetobacter, Erwinia, Flavobacterium, Kurthia, Lactobacillus,
Leuconostoc, Pediococcus and Streptococcus. As for molds, the most important plant-borne
genera are those that cause the damage of vegetables and fruits and these include, Fusarium,
Aspergillus, Botrytis, Alternaria etc. The commonly encountered genera of yeast in plant
products are the genus Saccharomyces, Rhodotorula and Torulas.
Food Utensils - The types of food borne microbes that are found in food utensils
depend on types of food to a large extent. The care of these utensils, storage and other factors
need to be taken into consideration. For example, utensils used in handling vegetables would
be expected to have organisms that are associated with vegetables. Also utensils which have
been cleaned with hot or boiling water will only as its micro-flora those organisms that are
able to withstand the management. Utensils stored in open place, where dust might gather
should be expected to have air-borne bacteria, yeast and molds.
Intestinal Tract of Man and Animals - Organisms usually found in the intestinal tract
of man are, Bacteroides, Escherichia, Lactobacillus, Proteus, Salmonella, Shigella,
Staphylococcus, and Streptococcus. Others include, Clostridium, Nitrobacteria, Enterobacter,
and Pseudomonas. These organisms, through fecal dissemination in man found their way
directly into soil and water. From soil they may find their way to food utensils. The most
encountered yeast in this case is the Candida.
Food Handlers - The organisms usually found on the hands and outer garments of
food handlers are a function of the environment and the habit of food handlers. Apart from
these, there are some bacteria that are explicitly associated with hands, nasal cavities and
mouth. These include Micrococcus and Staphylococcus. The genera Salmonella and Shigella
are basically found in the intestine of man. They may be deposited into foods and utensils by
food handlers. If sanitary practices are not followed by each individual. Yeast and Molds may
be found on the hands and garments of individuals depending upon their direct history.
Animal Feeds – The types of organisms to be found in animal feed would depend on
the source of the feeds, the treatment given to them damage micro-organisms, the container in
which they are stored and the like. Any one of the above mentioned genera of bacteria, yeast,
and mold may be found in animal feeds. The micro-organism, which is of particular
importance is Salmonella sp. which causes food poisoning.
Animal Hides - Generally organisms in soils, water, animal feeds, dust and faecal
matter are often found on the hides of animals. From the animal hides, the microbes may find
their ways into the air, hands of workers and directly into foods. Some members of the hide
flora find their way into the lymphatic system of slaughtered animals from which they
migrate into the muscle tissue of the slaughtered animals.
Air and Dust - Majority of genera of bacteria mentioned above except for some
pathogens are found in air and dust. Also, many genera of molds and yeast are found.
Bacillus and Micrococcus spp are some of the notable bacteria often found in air and dust
because of their ability to sustain aridity to changing degrees.
Conclusion
In addition to the natural micro-flora determined by type of plant or animal and
environmental conditions, every food may be contaminated from outside sources on the way
from the field to the processing plant, or during storage, transportation and distribution. There
are thousands of different types of micro-organisms everywhere in air, soil and water, and
consequently on foods, and in the digestive tract of animals and human beings. Fortunately,
the majority of micro-organisms perform useful functions in the environment and also in
some branches of food industry, such as production of wine, beer, bakery products, dairy
products etc. On the other hand, unwanted spoilage of foods is generally caused by micro-
organisms and contamination of food with pathogens causes food safety problems. Hence, it
is vital for the individuals to make sure that food is cleaned, before they are made use of.
When fruits and vegetables are purchased, they are washed, before they are cooked or
consumed.
Both foods of plant and animal origin normally carry a micro-flora on the surface of
their parts. Animals also have an intestinal micro-flora. Both animals and plants may also
become contaminated from outside sources. The inner and healthy tissues of plants and
animals, however, have been reported to contain a few living micro-organisms, or none.
The fruits and vegetables are harvested, milk is drawn, fish and other products are obtained
from natural waters, and animals are collected and slaughtered. All these carry their usual
micro-flora. After initial handling, further contamination initiates and it continues while the
product is being processed and prepared. Therefore, it can be stated that when individuals are
consuming various types of food items, they need to ensure, they are not spoiled or rotten, are
cleaned and proper ingredients and methods need to be made use of in their preparation. This
is essential to maintain good health, and the individuals are able to adequately manage food
items.
Bibliography
Oberg, C. (2012). History of Food Microbiology. Retrieved June 27, 2019 from
http://amyd.quimica.unam.mx/pluginfile.php/909/mod_resource/content/2/Oberg
%202012.%20%20History%20of%20Food%20Microb.pdf