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No.

Chromatography Mobile Phase Stationary Phase Best Applications Advantages Disadvantages


1 PC Paper Chromatography Liquid: Liquid: To separate mixture  Cheap  Cellulose
Water / alcohol Water moisture on ink  Simple paper is easily
cellulose solid support damaged
 Only works
for simple
mixture
2 TLC Thin Layer Chromatography Non-polar liquid: Solid: To separate mixture  Can be used  Sample must
Liquids hydrocarbons, Al2O3/SiO2 drugs in with corrosive be
Halogenoalkanes blood/urine/foods mixtures. concentrated
enough to be
seen as spots
3 GLC Gas Liquid Chromatography Inert gas: Liquid: To separate volatile  Rapid  Sample must
He / H2 / N2 gas Silicon oil on silica gel mixtures (drugs in separation be volatile /
solid support urine / pesticides )  Very small low B.p /
quantity of thermally
sample can be stable
analyzed  Cant separate
 Can be a good high B.p
tandem mixture
instrument (proteins,
with mass amino acids,
spectrometer carboxylic
acid) and
other
thermally
unstable
compound
such as
carbohydrates
.
4 HPLC High Performance Polar Liquid: Non-polar Liquid: To separate wider  Rapid  Can be used
liquid Chromatography Water / alcohol Silicon oil on silica gel spectrum of mixtures separation to separate
solid support such as  Very small carbohydrates
carbohydrates, amino quantity of and proteins.
acids, proteins, sample can be
carboxylic acids, analyzed
impurities

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