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Physioex Lab Report: Pre-Lab Quiz Results
Physioex Lab Report: Pre-Lab Quiz Results
Experiment Results
Predict Question
1 Predict Question: Which tube do you think will have the highest lipase activity?
Your answer: tube 6 (lipase, vegetable oil, bile salts, pH 9.0 buffer).
You correctly answered: both lipase activity and fatty acid release.
2 Why is it difficult to detect whether lipase is active in tube 5 (lipase, vegetable oil, bile
salts, pH 2.0 buffer)?
You correctly answered: The pH is already very low, so a decrease in pH might be difficult
to detect.
Experiment Data
Tube Reagent Reagent Reagent Reagent Time Temp pH
No 1 2 3 4 (min) (°C)
Vegetable pH 7.0
1 Li Bil lt
https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex8/act4/# 60 37 6 21 1/3
2/21/2021 PhysioEx Exercise 8 Activity 4
Vegetable pH 7.0
1 Lipase Bile salts 60 37 6.21
Oil Buffer
3 Which tube confirms that there is no lipase in bile salts or vegetable oil?
Your answer: tube 2 (lipase, vegetable oil, deionized water, pH 7.0 buffer).
Correct answer: tube 4 (deionized water, vegetable oil, bile salts, pH 7.0 buffer).
2 Which tube had the highest lipase activity? How well did the results compare with your
prediction? Discuss possible reasons why it may or may not have matched.
3 Explain why pancreatic lipase would be active in both the mouth and the pancreas.
https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex8/act4/# 2/3
2/21/2021 PhysioEx Exercise 8 Activity 4
4 Describe the process of bile emulsification of lipids and how it improves lipase activity.
https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex8/act4/# 3/3