Performed on: 

November 29, 2021

Performed by:   

Ma. Verel P. Abella

Bryle Trixthane Navora

Marriane Geil M. Siobal

Submitted to:
Mr. Rico Antonio Mariano

Experiment #2
Title: Bromination of Unsaturated Fatty Acids

I. Learning Objectives
 To test for the presence of Unsaturation among fatty acids.
 Determine how Bromine Test works.

II. Materials
A. Chemicals/Reagents
 Acetylene solution
 Cinnamic Acid
 Control Solution
 Bromine Solution

B. Glasswares
 Test tubes
 Test tube rack
 Dropper

III. Procedure
1. Place the test tubes in the rack and place the test samples in
separate test tubes then label them.
2. Drop bromine in the test samples and observe the color.

IV. Results and Observation

 Figure 1: Results of Bromination of Unsaturated Fatty Acids.

The Acetylene solution and Cinnamic Acid was not affected by the
color of the Bromine solution when it was added.

V. Discussion
Bromine test is used for the identification of unsaturation in a
chemical compound. The bromine has an orange-brownish color when in
a solution and it is lost when reacted with a chemical compound that is
alkene or alkyne. It can also react with aromatic compounds. The
decolorization of the bromine indicates the presence of unsaturation.

VI. Conclusion
The test was done to see if there was any unsaturation in the lipids.
When alkenes react with bromine, the double bond breaks, resulting in
each carbon being linked to a bromine atom. It produces a white liquid,
with the bromine losing its red-brown tint.

VII. References
https://www.youtube.com/watch?v=ylX940_f9aI